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Detection reagent card and kit

A technology for detecting reagents and reagent cards, which is applied to biochemical equipment and methods, and microbial measurement/inspection. It can solve the problems of inconvenient self-testing by subjects, complicated operation, and poor user experience, and achieves improved sensitivity and simple operation. , the effect of relaxed detection conditions

Active Publication Date: 2018-07-27
SHENZHEN HALDEX STC BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing detection kits are in the form of double, triple or quadruple, which must be refrigerated at low temperatures such as 2-8°C, and the storage conditions are strict; A chromogenic solution or stop solution, and use additional heating equipment to incubate the detection reagent or react at room temperature for more than 30 minutes
The use process of the existing test kits on the market is not only complicated to operate, but also requires additional professional equipment to be checked in a specialized medical institution, which is not convenient for subjects to self-test at home and other places, and the user experience is poor

Method used

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  • Detection reagent card and kit

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Experimental program
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Embodiment 1

[0028] This embodiment provides a detection reagent card. The reagent card is provided with 4 detection grooves for detection, and reagent blocks are arranged in the detection grooves; the reagent blocks are obtained by drying the reaction solution after dripping into the reaction membrane.

[0029] The reaction solution is one of: sialidase reaction solution, hydrogen peroxide reaction solution, leukocyte esterase reaction solution or pH reaction solution.

[0030] Specifically, the reaction membrane may be one of filter paper, chromatography membrane or glass fiber membrane.

[0031] Preferably, the reactive membrane is filter paper.

[0032] The preparation method of the reagent card is as follows: cut the filter paper into small pieces with a diameter of 5mm, put it into the detection tank adapted on the reagent card, and mix the sialidase reaction solution, hydrogen oxide reaction solution, leukocyte esterase reaction solution and pH Each 5 μL of the reaction solution wa...

Embodiment 2

[0048] The difference between this embodiment and embodiment 1 is: the content of the reaction solution components of each detection index is different, specifically as follows:

[0049] 1) The sialidase reaction liquid contains the following components: 0.1g / L 5-bromo-4-chloro-3 indolyl acetylneuraminic acid sodium salt, 30g / L trehalose, 0.01mol / L 2-morpholine Sulfonic acid buffer, 0.01% wt nitro blue tetrazolium chloride, 0.05% wt NP-40, 0.1% bovine serum albumin, 1% wt sodium sulfate, pH 4.5.

[0050] 2) The hydrogen peroxide reaction liquid contains the following components: 10U / mL horseradish peroxidase, 5%wt glycerol, 0.05%wt bovine serum albumin, 10mmol / L phosphate buffer, 0.1mmol / L 4-amino Antipyrine, 0.5mmol / L 2-hydroxy-3-m-toluidine propanesulfonate sodium, 20mmol / L β-mercaptoethanol, pH7.2.

[0051] 3) Leukocyte esterase reaction solution contains the following components: 0.1g / L pyrrole ester, 0.05g / L 1-diazo-2-naphthol-4 sulfonate, 18%wt PVP K-30, 0.05mol / L Citr...

Embodiment 3

[0054] The difference between this embodiment and embodiment 1 is: the content of the reaction solution components of each detection index is different, specifically as follows:

[0055] 1) The sialidase reaction liquid contains the following components: 0.3g / L 5-bromo-4chloro-3 indolyl acetylneuraminic acid sodium salt, 60g / L trehalose, 0.5mol / L 2-morpholine Sulfonic acid buffer, 0.1% wt nitro blue tetrazolium chloride, 0.5% wt NP-40, 1% bovine serum albumin, 5% wt sodium sulfate, pH 5.5.

[0056] 2) The hydrogen peroxide reaction liquid comprises the following components: 50U / mL horseradish peroxidase, 20%wt glycerol, 2%wt bovine serum albumin, 200mmol / L phosphate buffer, 0.5mmol / L 4-amino Antipyridine, 2mmol / L 2-hydroxy-3-m-toluidine propanesulfonate sodium, 100mmol / L DTT, pH7.0.

[0057] 3) Leukocyte esterase reaction solution contains the following components: 0.5g / L pyrrole ester, 0.3g / L 1-diazo-2-naphthol-4 sulfonate, 30%wt PVP K-30, 0.2mol / L Citrate buffer, 5% wt dec...

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Abstract

The invention discloses a detection reagent card and a kit and relates to the technical field of disease detection reagents. The detection reagent card disclosed by the invention is provided with a detection groove capable of detecting neuraminidase, catalase, leucocyte esterase or a pH (Potential of Hydrogen) value index. The invention further provides a bacterial vaginal disease multi-index self-detection kit comprising the detection card and a self-heating pad. The detection of each index of the reagent card disclosed by the invention is mutually independent and is one-step reaction; an additional developing solution or stopping solution is not needed; a subject only needs to add collected vaginal secretion into the reagent card and can detect the vaginal discharge; the self-heating padis introduced into the kit and heating equipment does not need to be additionally arranged, so that detection conditions are greatly widened and the reagent card is convenient for the subjects to useat home; domestic popularization of bacterial vaginosis detection is facilitated.

Description

technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a detection reagent card and a kit. Background technique [0002] The occurrence of bacterial vaginosis is due to the imbalance of vaginal flora and the reduction of Lactobacillus, which leads to the proliferation of other pathogens such as Gardnerella, various anaerobic bacteria, Campylovibrio, etc. It is actually based on Gardnerella. A mixed infection of the Lord. Bacterial vaginosis is one of the most common diseases in obstetrics and gynecology. The infection rate is between 15% and 50%, and it is easy to recur. 50% of women suffering from bacterial vaginosis are prone to premature delivery or low birth weight babies, and the children they give birth to are also likely to have various sequelae. [0003] The clinical diagnostic criteria for bacterial vaginosis were proposed by Amsel, including clue cells visible in vaginal secretions observed under a microscope, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/44C12Q1/34C12Q1/28
CPCC12Q1/28C12Q1/34C12Q1/44C12Q2326/96C12Q2334/30C12Q2334/52
Inventor 郑招荣刘金超黄灿
Owner SHENZHEN HALDEX STC BIOLOGICAL ENG CO LTD
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