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Carbon/fish-sourced collagen membrane composite material and preparation method of same

A composite material and collagen film technology, which is applied in the field of carbon/fish source collagen film composite materials and its preparation, can solve problems such as inability to fully utilize, cytotoxicity, and loss of the inherent shape of the material

Inactive Publication Date: 2018-07-27
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Nevertheless, freshwater fish collagen medical biomaterials, which have the functions of rapid hemostasis, wound repair and cavity filling, still have some disadvantages in terms of thermal stability, in vivo degradation stability, and in vivo material morphological stability compared with mammalian collagen. Obvious deficiencies: such as thermal denaturation at human body temperature, loss of inherent shape of the material after water absorption and swelling, and rapid degradation in the body, etc., which cannot fully exert its biological function
However, glutaraldehyde and isocyanate cross-linking agents have potential dangers such as chemical reagent residue and easy to cause cytotoxicity.

Method used

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  • Carbon/fish-sourced collagen membrane composite material and preparation method of same

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preparation example Construction

[0032] see figure 1 , the preparation method of the carbon / fish source collagen membrane composite material preparation method of one embodiment, comprises the following steps:

[0033] S110, extracting fish-derived collagen and configuring it into a fish-derived collagen solution.

[0034] In one embodiment, the solvent of the fish-derived collagen solution is acetic acid, the concentration of the fish-derived collagen solution is 1-4 mg / mL, and the volume of the fish-derived collagen solution is 10-40 ml.

[0035] In one embodiment, the fish-derived collagen is extracted from tilapia or cod skin.

[0036] In one embodiment, the preparation method of extracting fish-derived collagen is:

[0037] The tilapia skin is defatted by using 10%-15% n-butanol by volume percentage for 24h-48h to obtain the defatted fish skin. In one embodiment, the mass volume ratio w / v of tilapia fish skin and n-butanol is 1:20~1:40; the mass volume ratio w / v of defatted fish skin and NaOH aqueous ...

Embodiment 1

[0061] Take 15g of tilapia fish skin and degrease it with 10% (v) n-butanol (w / v=1:20) for 24h (replace once every 8h), wash it with distilled water and soak it in an aqueous NaOH solution with a concentration of 0.1mol / L for 24h ( w / v=1:20) to remove non-collagen components (replace every 8 hours), wash with water until neutral. Then add 0.5mol / L acetic acid solution (w / v=1:50) and 1% pepsin (750U, Sigma), extract with magnetic stirring at 4°C for 48h, centrifuge at 10000r / min for 30min, and the supernatant is the enzymatic solubility Collagen. Add NaCl to the supernatant to a final concentration of C(NaCl)=0.9mol / L, centrifuge, collect the precipitate and dissolve it in 0.5mol / L acetic acid solution, and use 0.02mol / L NaCl 2 HPO 4 The solution (pH8.6) was dialyzed for 24 hours, and the precipitate was collected by centrifugation, redissolved in 0.5 mol / L acetic acid solution, dialyzed with 0.1 mol / L acetic acid solution for 1 day, and then dialyzed with distilled water for...

Embodiment 2

[0064] Cod was used to extract collagen, and the preparation of collagen was carried out according to the method of Nagai and Suzuki (2000). Take 100g pollock skin and soak it in NaOH aqueous solution with a concentration of 0.1mol / L for 24h (1:20w / w) to remove non-collagenous components, wash it to neutrality and add acetic acid solution with a concentration of 0.5mol / L (1:20w / w) homogenate, extract with magnetic stirring at 4°C for 2 days, centrifuge at 10,000 r / min for 30 minutes, and the supernatant is acid-soluble collagen. Add NaCl to the supernatant until the final C(NaCl)=0.9mol / L, centrifuge, collect the precipitate and dissolve it in 0.5mol / L acetic acid solution, dialyze with 0.1mol / L acetic acid solution for 1d, then dialyze with distilled water for 2d, freeze Dried pollock skin acid soluble collagen.

[0065] The collagen is prepared into a solution, and 10mL of the fish-derived collagen solution is added to 1g of an acidic aqueous solution whose pH value is adj...

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Abstract

A preparation method of a carbon / fish-sourced collagen membrane composite material includes steps of: a) extracting fish-sourced collagen and preparing a fish-sourced collagen solution; b) adding thefish-sourced collagen solution to a phosphoric acid water solution, and adding carboxylated carbon nanotubes or carboxylation graphene oxide to the concentration of 0.01-0.05 wt%; c) adding EDC and NHS, and performing magnetic stirring at 4-10 DEG C overnight to perform a reaction; d) dialyzing the reaction product through a semipermeable membrane to remove non-reacted EDC and NHS, thus preparinga liquid mixture; e) performing ultrasonic dispersion to the liquid mixture to obtain an electrostatic membrane spinning raw solution; f) performing electrostatic membrane spinning to the electrostatic membrane spinning raw solution to obtain the carbon / fish-sourced collagen membrane composite material which is low in cellular toxicity and good in biocompatibility.

Description

technical field [0001] The invention relates to the field of marine biomedical materials, in particular to a carbon / fish source collagen membrane composite material and a preparation method thereof. Background technique [0002] Collagen is a structural protein in animal tissues, accounting for about 1 / 3 of the total protein. Collagen plays an important role in the development and migration of body cells, as well as the formation and function of body tissues. It is precisely because of the existence of collagen that animal tissues have a certain structure and mechanical properties such as tensile strength, tension, and viscoelasticity, which can support organs and protect the body. Collagen is widely used in food, cosmetics, biomaterials, tissue engineering and other fields. Traditional natural collagen mainly comes from mammalian skin and Achilles tendon tissue. However, due to concerns about zoonotic diseases and traditional religious beliefs, the biosafety of collagen ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/08A61K41/00D04H1/728D04H1/4382
CPCA61K41/0052A61K41/0057A61L27/08A61L27/24D04H1/4382D04H1/728
Inventor 李文军李杰秦松宋文奎
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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