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Method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes

A comprehensive extraction and camellia oleifera drying technology, applied in chemical instruments and methods, pharmaceutical formulations, steroids, etc., can solve the problems of difficult industrialization, large residual toxic substances, complex process, etc., and achieve convenient solvent recovery and easy operation Simple, simple process effect

Active Publication Date: 2018-07-20
GUANGXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The commonly used purification methods are: n-butanol extraction method, recrystallization method, ion exchange resin method, flocculant method, etc. These methods all have the disadvantages of low yield, large residual amount of toxic substances, and complicated process, so it is difficult to realize industrial change

Method used

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  • Method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes
  • Method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes
  • Method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Such as Figure 1-5 As shown, 0.5 kg of non-defatted Camellia oleifera powder was added to 5 L of 70 % ethanol, continuously extracted at 70 ° C for 3 h, and filtered. Filter the filtrate through a ceramic membrane, add 95% ethanol to the retentate for alcohol precipitation to obtain polysaccharides, and concentrate the permeate under reduced pressure until it has no alcohol smell. The pH of the 0.1% HCl solution concentrate is 4.0, and AB-8 macroporous resin is used for adsorption. The macroporous resin after adsorption was washed with water until the effluent was colorless, then eluted with 0.02% NaOH solution, and the polyphenols were analyzed, then washed with water until the effluent was neutral, and finally the soap was treated with 80% ethanol solution. The element is eluted. The NaOH eluate was collected and adjusted to pH = 5.0 with 0.1% HCl solution, concentrated by nanofiltration membrane, and vacuum-dried to obtain 9.6 g reddish-brown polyphenols with a pu...

Embodiment 2

[0033] Such as Figure 1-5 As shown, 1.0 kg of non-defatted Camellia oleifera powder was added to 12 L of 60% ethanol, continuously extracted at 80°C for 4 h, and filtered. Filter the filtrate through a ceramic membrane, add 95% ethanol to the retentate for alcohol precipitation to obtain polysaccharides, and concentrate the permeate under reduced pressure until it has no alcohol smell. with 0.5% H 2 SO 4 The pH of the solution concentrate is 6, and ADS-21 macroporous resin is used for adsorption. The macroporous resin after adsorption is washed with water until the effluent is colorless, then eluted with 0.03% NaOH solution, and the polyphenols are analyzed, then washed with water until the effluent is neutral, and finally the soap is treated with 90% ethanol solution. The element is eluted. The NaOH eluate was collected and washed with 0.5% H 2 SO 4 Solution Adjust the solution to pH = 4.5, concentrate it with nanofiltration membrane, and use freeze-drying to obtain 20...

Embodiment 3

[0035] Such as Figure 1-5 As shown, 10.0 kg of non-defatted Camellia oleifera powder was added to 150 L of 75% ethanol, continuously extracted at 85°C for 4 h, and filtered. Filter the filtrate through a ceramic membrane, add 95% ethanol to the retentate for alcohol precipitation to obtain polysaccharides, and concentrate the permeate under reduced pressure until it has no alcohol smell. with 1 % H 2 SO 4 The pH of the solution concentrate is 5, and AB-8 macroporous resin is used for adsorption. The macroporous resin after adsorption is washed with water until the effluent is colorless, then eluted with 0.04% NaOH solution, and the polyphenols are analyzed, then washed with water until the effluent is neutral, and finally the soap is treated with 85% ethanol solution. The element is eluted. The NaOH eluate was collected and washed with 1% HO 2 SO 4 Solution Adjust the solution to pH=5.0, concentrate it with nanofiltration membrane, and use spray drying to obtain 198.6 g...

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Abstract

The invention discloses a method for comprehensively extracting saponin and polyphenol from Camellia oleifera Abel. cakes. The method comprises the following steps: (1) extracting the saponin and thepolyphenol in the Camellia oleifera Abel. cakes by utilizing an ethanol-water system; (2) filtering an extracting solution through a ceramic membrane; (3) after concentrating ceramic membrane permeated liquid, regulating the pH (Potential of Hydrogen); then adsorbing by adopting macroporous resin and eluting by utilizing water, an NaOH solution and an ethanol solution respectively; (4) regulatingthe pH of an NaOH eluting solution and concentrating by a nano-filtration membrane; drying to obtain the polyphenol; (5) concentrating an ethanol eluting solution until no alcohol flavor is formed, and drying to obtain the saponin. According to the method disclosed by the invention, a membrane separation and macroporous resin adsorption technology is adopted, and the influences of macromolecular compounds including polysaccharides, protein, grease, pigments and the like, and impurities including salt and the like in a traditional method on saponin and polyphenol separation and purification areovercome; the method has the characteristics of simple equipment operation, moderate conditions, easiness for controlling, simple process route, high product purity and the like.

Description

technical field [0001] The invention relates to a method for comprehensively extracting saponin and polyphenols from camellia oleifera, belonging to the field of comprehensive utilization and development of camellia oleifera. Background technique [0002] Camellia ( Camellia oleifera Abel.) is a plant of the genus Camellia in the family Theaceae. Because of its large oil yield and high nutritional value, it is named as the four major woody edible oil source tree species along with oil palm, olive and coconut. Camellia oleifera seed residue after oil extraction is Camellia oleifera. According to statistics, 1 million tons of camellia oleifera seeds will produce about 680,000 tons of camellia oleifera. Because camellia oleifera tastes bitter and pungent, it is extremely harmful to cold-blooded animals. It is often processed by incineration or discarded, resulting in a great waste of resources. Experimental data show that camellia oleifera contains rich saponin, polysacchari...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K36/82C08B37/00C07G99/00
CPCA61K36/82C07H1/08C07H15/256C07J63/008C08B37/0003C07G99/00
Inventor 覃江克唐文迪黎国庆黎青黄俊
Owner GUANGXI NORMAL UNIV
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