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Silk fibroin/keratin composite porous material and preparation method thereof

A technology of silk fibroin and porous materials, which can be used in medical science, tissue regeneration, prosthesis, etc. It can solve the problems of unfavorable biocompatibility of silk fibroin porous materials, complicated experimental procedures, allergic reactions, etc., and achieve great development potential. , the process is simple, the effect of good biocompatibility

Inactive Publication Date: 2018-07-13
NANTONG TEXTILE & SILK IND TECH RES INST +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the porous material formed by freeze-drying the pure silk fibroin solution has a random coil structure and is easily soluble in water. Therefore, the existing preparation methods of silk fibroin porous materials basically need to add some additives to induce it to form a water-insoluble material. In the subsequent technological process, it needs to be removed, which makes the experimental process too complicated. In addition, most of the additives are organic solvents, which have certain toxicity, which is not conducive to the biocompatibility of silk fibroin porous materials, especially when After these materials are implanted in the human body, it is easy to cause allergic reactions

Method used

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  • Silk fibroin/keratin composite porous material and preparation method thereof
  • Silk fibroin/keratin composite porous material and preparation method thereof
  • Silk fibroin/keratin composite porous material and preparation method thereof

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preparation example Construction

[0032] The invention provides a silk fibroin / keratin composite porous material, which takes silk fibroin and keratin as main raw materials, mixes a certain concentration of silk fibroin and keratin solutions in a certain proportion, then adds an appropriate amount of glycerin, and then The silk fibroin / keratin composite porous material is obtained by freezing and vacuum drying. The prepared composite porous material has a porous structure with uniform pore size, the pore size is between 100µm and 500µm, the porosity is between 80% and 95%, the compressive strength is between 1.5MPa and 3.5MPa, and it is water insoluble. The preparation method of the above-mentioned composite porous material, the steps are as follows:

[0033] (1) Use a specific method (reduction method or alkaline method, etc.) to dissolve a certain amount of wool fiber and then filter it, and then use a dialysis bag with a molecular weight cut-off of 2000Da~20000Da for dialysis for 1~5 days. 6000-20000 polye...

Embodiment 1

[0048] This implementation case shows a preparation method of silk fibroin / keratin composite porous material, including:

[0049] (1) Use 1%~5% NaOH solution to dissolve a certain amount of wool fiber and then filter it, then use a dialysis bag with a molecular weight cut-off of 2000Da~20000Da for dialysis for 1~5 days. The diol solution was concentrated to obtain a wool keratin solution with a mass fraction of 6%.

[0050] (2) Degumming and dissolving the natural silk, dialysis and centrifugation according to the method in step (1), and concentration with a PEG solution with a molecular weight of 20,000 to obtain a silk fibroin solution with a mass fraction of 6%.

[0051] (3) Take a certain amount of silk fibroin solution and wool keratin solution, prepare a mixed solution in a 24-well plate according to the ratio of silk fibroin protein: wool keratin quality = 6:4, and add glycerin The quality of the double protein is 30% of the total mass.

[0052] (4) The mixed solution...

Embodiment 2

[0054] This implementation case shows a preparation method of silk fibroin / keratin composite porous material, including:

[0055] (1) Prepare 100ml of solution with 7M urea, 0.08M Na2S and 0.8g sodium dodecyl sulfate (SDS), then add 5g of wool fiber, and conduct water bath treatment at a water bath temperature of 50°C for 8 hours, then filter, and then use molecular weight cut-off Dialyze a 2000Da-20000Da dialysis bag for 1-5 days, then concentrate with a polyethylene glycol (PEG) solution with a molecular weight of 20000 to obtain a wool keratin solution with a mass fraction of 8%.

[0056] (2) Degumming and dissolving the natural silk, dialysis and centrifugation according to the method in step (1), and concentration with a PEG solution with a molecular weight of 20,000 to obtain a silk fibroin solution with a mass fraction of 8%.

[0057] (3) Take a certain amount of silk fibroin solution and wool keratin solution, prepare a mixed solution in a 24-well plate according to th...

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Abstract

The invention discloses a silk fibroin / keratin composite porous material and a preparation method of the composite porous material. The composite porous structure material with uniform pore distribution and a controllable size is prepared by taking silk, animal wool, human hair and poultry and bird feather as major raw materials. The material is natural, safe, nontoxic, good in biocompatibility, suitable for extensive application in the fields of muscle tendons, nerves, blood vessels, skin and soft and hard bone tissue engineering and the like, complies with the development and interdisciplinary planning of the national biomedical industry and belongs to an emerging industry field with great development potentials and wide market prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a silk fibroin / keratin composite porous material and a preparation method thereof. Background technique [0002] In recent years, human health problems caused by various factors such as food safety, environmental degradation, unhealthy living habits and population aging have become increasingly prominent. The incidence of various diseases such as tendon, nerve, blood vessel, skin and bone damage and cancer remains high. Taking soft and hard bone diseases as an example, bone defects caused by factors such as congenital defects or infection, trauma and tumor resection Diseases, especially common, bring great pain and burden to patients. The loss or dysfunction of these vital organs is one of the major hazards to human health. Traditional surgical treatment methods and radiotherapy and chemotherapy methods are usually difficult to effectively achieve the p...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/56A61L27/58A61L27/50A61L27/60
CPCA61L27/26A61L27/507A61L27/56A61L27/58A61L27/60A61L2430/02A61L2430/06A61L2430/10A61L2430/32C08L89/00
Inventor 李刚田焰宽郑兆柱王晓沁
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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