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Ginkgo leaf extract and extraction method thereof

A technology of extract and ginkgo biloba, applied in the field of pharmacy, can solve problems such as the inability to achieve the best curative effect level, and achieve the effects of improving the curative effect level of the drug and predicting good performance

Inactive Publication Date: 2018-07-13
ZHEJIANG WANBANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Ginkgo biloba extract contains terpene lactone components. The present invention provides more effective ginkgo leaf extract by determining the ratio of different terpene lactone components, thereby overcoming the problem that ginkgo biloba preparations on the market only simply control flavonol glycosides. The content of two major categories of components, terpene lactone and terpene lactone, causes the defect that the best curative effect level cannot be reached, thereby improving the drug curative effect level of ginkgo biloba preparations

Method used

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  • Ginkgo leaf extract and extraction method thereof
  • Ginkgo leaf extract and extraction method thereof
  • Ginkgo leaf extract and extraction method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0054] 1. Preparation of Ginkgo Biloba Extract

[0055] Get the dried ginkgo fallen leaves and pulverize them with a pulverizer. Accurately weigh 50g of the powder and place it in a 1000mL round bottom flask, add a certain proportion of ethanol water to soak for 1h, then extract twice under heat reflux, and combine the two extracts. The extract was shaken up, centrifuged, and filtered through a membrane filter.

[0056] 2. Process design

[0057] The process evaluation index set in the present invention is the ratio of terpene lactones (sesquiterpene / diterpenes) (Y1), the extraction amount (Y2) of total flavonoids in a unit medicinal material, the purity of flavonoids (Y3) and the purity of terpene lactones ( Y4).

[0058] The effects of three process parameters, ethanol concentration, liquid-solid ratio and extraction time, on the extraction process of Ginkgo biloba were investigated. The quantitative relationship between the key process parameters and process evaluation ...

Embodiment 2

[0085] Evaluation of antithrombotic effect of different ratios of terpene lactones

[0086]Experimental method: 2dpf wild-type AB strain zebrafish were cultured in six-well plates, 30 zebrafish per well, and given "BB:(GA+GB+GC)=1:10" and "BB:(GA+GC) respectively in water solution. GB+GC)=1:5", "BB:(GA+GB+GC)=1:2", "BB:(GA+GB+GC)=1:1", "BB:(GA+GB+ GC)=2:1" (dosing concentration is 500 μg / mL), positive control drug clopidogrel 7.5 μg / mL, set normal control group and model control group at the same time, each well volume is 3mL. Except for the normal control group, the rest of the experimental groups were given phenylhydrazine in water solution to induce the zebrafish thrombus model. Ginkgo biloba dripping pills and phenylhydrazine were used to treat the zebrafish for 18 hours, and then stained with o-dianisidine. After staining, 12 zebrafish were randomly selected from each experimental group, photographed and collected data under a dissecting microscope, and the staining inte...

Embodiment 3

[0091] Evaluation of antithrombotic effect of Ginkgo biloba extracts prepared in different ways

[0092] The experimental procedure is the same as that in Example 2, except that the experimental group was given water-soluble administration of "commercially available ginkgo biloba preparation 1", "commercially available ginkgo biloba preparation 2", "commercially available ginkgo biloba preparation 3", and "new process group extract" (The administration concentration of the extract is 500 μg / mL), and the positive control drug clopidogrel is 7.5 μg / mL.

[0093] The experimental results show that: the positive drug and the four experimental groups all have a preventive effect on the formation of zebrafish thrombus induced by phenylhydrazine, and can significantly increase the staining intensity of red blood cells in the heart of the zebrafish, and the antithrombotic effect of the new process group is significantly stronger than that of the commercially available Ginkgo biloba pre...

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Abstract

The invention belongs to the technical field of pharmacies, relates to a traditional Chinese medicine raw material extract and an extraction method thereof, particularly relates to a ginkgo leaf extract and an extraction method thereof. The extraction method for the extract comprises the following steps: smashing dried ginkgo leaves with a smashing machine; and performing reflux extraction on thesmashed ginkgo leaves by ethanol to obtain the ginkgo leaf extract. By adopting the extraction method, the effective component content is controlled effectively, the medicinal effect of the medicine is improved.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to a traditional Chinese medicine raw material extract and an extraction method thereof, in particular to a ginkgo leaf extract and an extraction method thereof. Background technique [0002] Since the 1960s, plant extracts of Ginkgo biloba have been widely used in cardiovascular and cerebrovascular diseases and peripheral blood circulation diseases in many countries, such as EU countries, Japan and South Korea. At present, there are more than 40 related ginkgo leaf extract preparations on the market, and as of 2013, there are more than 100 domestic manufacturers. However, different ginkgo leaf preparations from different manufacturers have different clinical efficacy. Currently, domestically recognized ingredients related to efficacy are terpenoid lactones (gingkgolides A, B, and C, and bilobalide) and glycosides of flavonoids (quercetin, kaempferol, and isorhamnetin). Patent No. 5,389,370 j...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/16A61P9/10A61P7/02A61K127/00
CPCA61K36/16A61K2236/15A61K2236/333A61K2236/39
Inventor 赵守明范骁辉陈莉陆晓燕王青青张建兵
Owner ZHEJIANG WANBANG PHARMA
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