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Fluorescent-Raman dual probe material for detecting zinc ions and preparation method thereof

A dual-probe, zinc ion technology, applied in the field of analysis and detection, can solve the problems of cumbersome processing process, high drug cost, long analysis time, etc., and achieve the effects of simple process, convenient popularization and use, and high detection sensitivity

Active Publication Date: 2018-07-06
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, fluorescence analysis combined with its imaging advantages has developed a large number of Zn 2+ fluorescent sensors, however, which require Zn 2+ Linking organic fluorophores to form ligands makes the pretreatment process very cumbersome, the analysis time is long and the drug cost is high, and it is difficult to distinguish Zn 2+ and Cd 2+ Two structurally similar ions

Method used

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  • Fluorescent-Raman dual probe material for detecting zinc ions and preparation method thereof
  • Fluorescent-Raman dual probe material for detecting zinc ions and preparation method thereof
  • Fluorescent-Raman dual probe material for detecting zinc ions and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] (1) Preparation of gold nanoparticles

[0036] Weigh 10mgHAuCl 4 Dissolve in 100ml of double-distilled water, heat to boiling, quickly inject 1ml of 1% sodium citrate under vigorous stirring, maintain continuous reflux for 30min, and cool to room temperature. A wine-red solution was prepared and stored at 4°C to obtain AuNPs with a colloidal concentration of 0.5nmol / L and a diameter of 50nm.

[0037] (2) Preparation of MDPA bipyridine compound.

[0038] First, 2.24g of N-tert-butoxycarbonylethylenediamine and 4.6g of chloromethylpyridine hydrochloride were dissolved in 50mL of 5mol / L NaOH aqueous solution and stirred overnight; 2 SO 4 Dry and purify by rotary evaporation; the crude product is further purified by silica gel column using dichloromethane / methanol (95:5) as eluent to give the product N-tert-butoxycarbonyl-N,N-diaminoethylenediamine , It is a brown-yellow oily substance.

[0039] Next, 1.9 g of the above compound was dissolved in 15 mL of dichloromethan...

Embodiment 2

[0046] (1)Zn 2+ Chelation of MDPA-AuNPs probes

[0047] figure 2 It is the ultraviolet spectrogram of functionalized gold nanoparticles in Example 1 with the increase of zinc ion concentration (0-10 μmol / L).

[0048] The ultraviolet plasmon absorption peak of the prepared gold nanoparticles is 520nm, figure 2 It can be seen that with the addition of zinc ions, chelation with bipyridine compounds occurs, resulting in self-agglomeration of gold nanoparticles. At the same time, as the concentration of zinc ions reaches 0.1 μmol / L, a new plasma absorption peak appears at 680nm. At the same time according to image 3 According to the results of transmission electron microscopy, after adding 0.1 μmol / L zinc ions, the modified gold nanoparticles agglomerated. The agglomeration can also enhance the Raman enhancement factor, which is of great significance for Raman probes.

[0049] (2)Zn 2+ fluorescence detection

[0050] Such as Figure 4 As shown, 4ml of nanoprobes were pla...

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Abstract

The invention belongs to the technical field of analysis and detection, and particularly discloses to a fluorescent-Raman dual probe material for detecting zinc ions and a preparation method thereof.The preparation method comprises the following steps: (1) firstly, performing a reaction on N-tert-butoxycarbonylethylenediamine and chloromethylpyridine to produce an N-Boc-protected dipyridine compound, and then removing a Boc protecting group by hydrofluoric acid to obtain N, N-dimethylpyridineethylenediamine; (2), performing an amidation reaction on the N, N-dimethylpyridineethylenediamine andmercaptoacetic acid to obtain bipyridinemercaptoacetamide; (3), with a self-assembly method, modifying the bipyridinemercaptoacetamide onto the surfaces of gold nanoparticles, incubating at room temperature, and centrifuging to obtain the fluorescent-Raman dual probe material for detecting the zinc ions. The preparation method is simple and easy to implement; the obtained fluorescent-Raman dual probe material can specifically recognize the zinc ions, and is high in sensitivity and fast in response; the fluorescent-Raman dual probe material has a potential application prospect in the fields ofbioimaging, environment and the like.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a fluorescence-Raman double probe material for detecting zinc ions and a preparation method thereof. Background technique [0002] Zinc is the second most abundant transition metal in the human body. It is an essential trace element for the human body. It not only plays a key role in many physiological processes, such as gene expression, cell metabolism, and DNA recognition, but also can promote human growth and development, Enhance immunity and regulate the action of various enzymes and receptors. The lack of zinc ions will lead to dwarfism, mental retardation, fetal growth retardation and other symptoms, so it is of great significance to find a method for in situ real-time detection of zinc ion concentration. [0003] At present, the detection methods of zinc ions mainly include fluorescence analysis, atomic absorption spectrophotometry and electroch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D213/38C07F1/12C09K11/06G01N21/65G01N21/64
CPCC07D213/38C07F1/005C09K11/06C09K2211/188G01N21/6428G01N21/658Y02P20/55
Inventor 李丹马亚丹段化珍邓维
Owner SHANGHAI INST OF TECH
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