Ellagic acid reagent and preparation method thereof as well as activated partial thromboplastin time (APTT) determination reagent and APTT kit
A preparation method and technology of ellagic acid, applied in the direction of biological testing, material inspection products, etc., can solve the problems of loss of activity, precipitation, and affecting the quality of domestic reagents, and achieve good stability and simple and fast process
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Embodiment 1
[0045] This embodiment provides an ellagic acid reagent, which is prepared by the following preparation method:
[0046] (1) Add 0.4 mmol ellagic acid into calcium-free purified water, and stir rapidly to make it evenly dispersed.
[0047] 0.5 mmol sodium acetate was added.
[0048] Quickly add 1 mmol Tris-hcl within 10 minutes after adding ellagic acid to adjust the pH to 7.1±0.1.
[0049] Add 0.025mmol iron ion.
[0050] Arginine hydrochloride 1.5% and glycine 1.5% were added.
[0051] Add the preservative sodium azide.
[0052] (2) Preparation of emulsified phospholipids: Add calcium-free purified water at 5°C to 0.32 g / L rabbit cephalin, mix and emulsify in an ice bath at 5°C to obtain emulsified phospholipids.
[0053] (3) Add the emulsified phospholipid into the buffer solution in a specific ratio, stir and mix quickly to obtain a yellow uniform dispersion, that is, the ellagic acid reagent.
[0054] This embodiment also provides a reagent for measuring activated pa...
Embodiment 2
[0056] This embodiment provides an ellagic acid reagent, which is prepared by the following preparation method:
[0057] (1) Add 0.2 mmol of ellagic acid into calcium-free purified water, and stir rapidly to make it evenly dispersed.
[0058] Add 0.4 mmol Tris-base.
[0059] Quickly add 0.8mmol Hepes within 10 minutes after adding ellagic acid to adjust the pH to 7.1±0.1.
[0060] Add 0.02 mmol of magnesium ions.
[0061] Add alanine.
[0062] Add preservative proclin300.
[0063] (2) Preparation of emulsified phospholipids: Add calcium-free purified water at 3°C to 0.3 g / L rabbit cephalin, mix and emulsify in an ice bath at 3°C to obtain emulsified phospholipids.
[0064] (3) Add the emulsified phospholipid into the buffer solution in a specific ratio, stir and mix quickly to obtain a yellow uniform dispersion, that is, the ellagic acid reagent.
[0065] This embodiment also provides a reagent for measuring activated partial thromboplastin time, which includes the ab...
Embodiment 3
[0067] This embodiment provides an ellagic acid reagent, which is prepared by the following preparation method:
[0068] (1) Add 0.5 mmol of ellagic acid into calcium-free purified water, and stir rapidly to make it evenly dispersed.
[0069] 0.8 mmol sodium hydroxide was added.
[0070] Quickly add about 0.5ml of acetic acid within 10 minutes after adding ellagic acid to adjust the pH to 7.1±0.1.
[0071] Add 0.03 mmol manganese ions.
[0072] Add histidine and glutamic acid.
[0073] Add the preservative gentamicin.
[0074] (2) Preparation of emulsified phospholipids: Add calcium-free purified water at 8°C to 0.35 g / L rabbit cephalin, mix and emulsify in an ice bath at 8°C to obtain emulsified phospholipids.
[0075] (3) Add the emulsified phospholipid into the buffer solution in a specific ratio, stir and mix quickly to obtain a yellow uniform dispersion, that is, the ellagic acid reagent.
[0076] This embodiment also provides a reagent for measuring activated partia...
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