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A kind of active ingredient of Tibetan danshen tanshinone and its extraction method and application

A technology of active ingredients and extraction methods, which is applied in the field of active ingredients and extraction of tanshinones from Tibetan salvia miltiorrhiza, can solve the problems of low purity of active ingredients, cumbersome operation, and long extraction time, and achieves improved purity and extraction efficiency, simple operation, and shortened separation the effect of time

Inactive Publication Date: 2021-04-13
CHENGDU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides an active ingredient of Tibetan Danshen tanshinone and its extraction method and application, which can effectively solve the problems of low purity of the active ingredient extracted by the existing extraction method, cumbersome operation, and long extraction time.

Method used

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  • A kind of active ingredient of Tibetan danshen tanshinone and its extraction method and application
  • A kind of active ingredient of Tibetan danshen tanshinone and its extraction method and application

Examples

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Embodiment 1

[0021] A method for extracting active ingredients of Tibetan Danshen tanshinones, comprising the following steps:

[0022] (1) Ultrasonic extraction: Dry and pulverize Tibetan Salvia miltiorrhiza, pass through a 50-mesh sieve, add 3 times the weight of Tibetan salvia miltiorrhiza to the powder and soak in deionized water for 2 hours, then add 6 times the weight of Tibetan salvia miltiorrhiza after soaking and Volume fraction is 90% ethanol, ultrasonic extraction 60min, filter, get the first filtrate and the first filter residue, add 6 times of the first filter residue weight and volume fraction is 90% ethanol ultrasonic extraction 60min to the first filter residue, filter, obtain Combine the first filtrate and the second filtrate for the second filtrate and the second filter residue, wherein the ultrasonic conditions are protected from light, the ultrasonic frequency is 35kHz, the temperature is 40°C, and the power is 250W;

[0023] (2) Preparation of fat-soluble extract: Conc...

Embodiment 2

[0026] A method for extracting active ingredients of Tibetan Danshen tanshinones, comprising the following steps:

[0027] (1) Ultrasonic extraction: Dry and pulverize Tibetan Salvia miltiorrhiza, pass through a 50-mesh sieve, add 3 times the weight of Tibetan salvia miltiorrhiza to the powder and soak in deionized water for 2 hours, then add 8 times the weight of Tibetan salvia miltiorrhiza after soaking and Ethanol with a volume fraction of 90%, ultrasonically extracted for 90 minutes, filtered to obtain a filtrate, wherein the ultrasonic conditions were protected from light, the ultrasonic frequency was 35kHz, the temperature was 40°C, and the power was 250W;

[0028] (2) Preparation of fat-soluble extract: the filtrate obtained in step (1) is concentrated under reduced pressure to a 1:1 concentrated solution, an equal volume of ethyl acetate is added to the concentrated solution, and the dark extraction is performed 3 times, the extract is collected, and the extract is redu...

Embodiment 3

[0031] A method for extracting active ingredients of Tibetan Danshen tanshinones, comprising the following steps:

[0032] (1) Ultrasonic extraction: dry and pulverize Tibetan Salvia miltiorrhiza, pass through a 50-mesh sieve, add 3 times the weight of Tibetan salvia miltiorrhiza to the powder and soak in deionized water for 2 hours, then add 7 times the weight of Tibetan salvia miltiorrhiza after soaking and Volume fraction is 60% ethanol, ultrasonic extraction 80min, filter, obtain the first filtrate and the first filter residue, add 7 times of the first filter residue weight and volume fraction to the first filter residue in the ethanol ultrasonic extraction of 80min, filter, obtain Combine the first filtrate and the second filtrate for the second filtrate and the second filter residue, wherein the ultrasonic conditions are protected from light, the ultrasonic frequency is 35kHz, the temperature is 40°C, and the power is 250W;

[0033] (2) Preparation of fat-soluble extract...

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Abstract

The invention discloses an active ingredient of Tibetan salvia tanshinone and its extraction method and application. The extraction method comprises the following steps: drying and crushing Tibetan salvia miltiorrhiza, then soaking in water, and adding 6-8 times the amount of 60-60 90% ethanol, ultrasonic extraction for 60-90min, extraction 1-2 times; Concentrate the ultrasonic extract, then extract with ethyl acetate, collect the extract, concentrate under reduced pressure to obtain a fat-soluble extract, and then extract the extract Dissolved to the corresponding concentration, separated and purified by medium-pressure preparative chromatography. The method of the invention is simple to operate, can effectively separate and purify target components, greatly improves its purity and extraction efficiency, and can fully exert its medicinal value.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients of Tibetan danshen, and in particular relates to an active ingredient of Tibetan danshen tanshinones, an extraction method and an application thereof. Background technique [0002] The scientific name of Tibetan Salvia is Salvia castanea Diels f.tomentosa Stib, which is a variety of Salvia castanea Diels (Salvia castanea Diels f. tomentosa Stib), which is produced in Nyingchi, Tibet. In December 2005, Sage tomentosa was included in the local medicinal material standards by the Tibet Autonomous Region Food and Drug Administration, and named "Tibetan Salvia". Studies have shown that Tibetan Salvia contains fat-soluble and water-soluble components. The fat-soluble components contain tanshinone diterpene fat-soluble chemical components such as cryptotanshinone, tanshinone I and tanshinone IIA, and have anti-myocardial ischemia, improvement of myocardial infarction, anti-thrombosi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J73/00A61K31/58A61P9/00A61P9/10A61P7/02A61P25/28
CPCA61K31/58C07J73/003
Inventor 付强梁峻铭杨龙飞徐玲曾妮
Owner CHENGDU UNIV
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