Preparation method and application of anti-lipid peroxide

Abstract

The invention discloses a preparation method of anti-lipid peroxide. The preparation method comprises the following steps that a dry monascus fermentation sample is taken, an extraction solvent is added into the sample, extracting is carried out through a lixiviating method so that a rough extraction product can be obtained, then, the rough extraction product is eluted through column chromatography so that purified eluant can be obtained, silica gel thin layer chromatography is conducted on the purified eluant, eluant with the same silica gel thin layer chromatography results is combined, column chromatography elution is carried out again, and then the anti-lipid peroxide is obtained. Monascus serves as a raw material to prepare the anti-lipid peroxide for the first time, the rough extraction product of the monascus sample is extracted firstly, optimal column chromatography eluant is screened out according to the activity distribution situation and the solubility in different solvents, and the anti-lipid peroxide is effectively separated and purified. The preparation method is simple and easy to realize, the consumed time is short, the raw material is easy to get and low in cost, and batch production of the anti-lipid peroxide is achieved.

Description

Technical field [0001] The invention relates to the technical field of sterol compound extraction, in particular to a preparation method and application of anti-lipid peroxide. Background technique [0002] Free radicals refer to atoms or molecules with unpaired electrons in the outer orbital. They are extremely unstable and very lively. They are the products of normal aerobic metabolism of cells, and most of them exist in the form of reactive oxygen species (ROS). Once free radicals are generated, most of the free radicals will be eliminated by the antioxidant defense system (enzymes and non-enzyme molecules) in the cell. The maintenance of the balance between free radicals and the antioxidant defense system is an essential condition for the normal functioning of organisms. ROS is beneficial to the body at low or medium concentrations. It is involved in the signal transduction and regulation of cells in physiological processes. However, when ROS is produced too much or the fun...

AI Technical Summary

Problems solved by technology

At present, there are few methods for preparing anti-lipid peroxides. The existing reaction conditions for anti-lipid peroxides are still not gentle enough, and it takes a long time to prepare. still not high enough

Images