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Sugarcane endogenous burkholderia sp. CZ08152 and application thereof

A technology for growing Burkholderia and Burkholderia, which is applied to endophytic Burkholderia CZ08152 in sugarcane and its application field, and can solve the problem of poor soil water retention, poor fertilizer retention, and fertilizer Problems such as low utilization rate can achieve the effect of improving plant resistance to adversity stress, promoting crop growth, and enhancing resistance

Active Publication Date: 2018-06-12
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, more than 90% of the sugarcane land in my country's main sugarcane production areas is dry slope land, the soil water and fertilizer retention capacity is poor, and the utilization rate of chemical fertilizers is very low.

Method used

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  • Sugarcane endogenous burkholderia sp. CZ08152 and application thereof
  • Sugarcane endogenous burkholderia sp. CZ08152 and application thereof
  • Sugarcane endogenous burkholderia sp. CZ08152 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation, screening and identification of strains

[0029] 1. Isolation of strains

[0030] Test material: stem base tissue of Xintaitang No. 22, a sugarcane variety planted in the suburb of Chongzuo City, Guangxi Zhuang Autonomous Region.

[0031] The sugarcane stems were taken, soaked in 70% alcohol for 30s on the ultra-clean workbench, rinsed with sterile water for 3 times, then soaked in 0.1% mercuric chloride for 1min, soaked in 70% alcohol for 30s, and rinsed with sterile water for 5 times. Weigh 1g of surface-sterilized tissue pieces, soak them in 10mL of phosphate buffer solution, grind them in a sterile mortar, and let them stand for 5min. Take the supernatant and make a 10-fold gradient dilution with sterile water, and take 100μL of the original solution and the diluted solution respectively. Spread the Ashby nitrogen-free medium plate and culture at 28°C for 2-3 days until a single colony grows. After the colony grows, strains are purified by streaking on ...

Embodiment 2

[0060] Effects of Burkholderia CZ08152 Strain on Nitrogen Fixation and Growth Promotion of Sugarcane

[0061] The cultivation medium is ordinary straw-based seedling raising medium, and 10mg ( 15 NH 4 ) 2 SO 4 , into plastic basins. After activation, the Burkholderia CZ08152 strain was cultured overnight on LB liquid medium, and the bacterial cells were collected by centrifugation, washed twice by suspension and centrifugation with sterile water, and then diluted to 1-2×10 per ml 8 cells, that is, the inoculum suspension of Burkholderia CZ08152 was obtained. The tissue-cultured seedlings of the sugarcane variety Xintaitang No. 22 (ROC22) were selected as the inoculation object, and the sugarcane tissue-cultured seedlings with consistent growth after hardening were transplanted into plastic pots equipped with cultivation substrates, and each pot was poured into the above-mentioned obtained Birke Holly. Inoculate 100mL of the germ suspension of Erdella spp. CZ08152, and use...

Embodiment 3

[0066] Promoting Effect of Burkholderia CZ08152 Strain on the Growth of Maize Seedlings

[0067] After activation, Burkholderia CZ08152 was cultured overnight on LB liquid medium, and the bacterial cells were collected by centrifugation, washed twice by suspension and centrifugation with sterile water, and then diluted to 1-2×10 per ml 8 cells as the inoculum suspension, namely the Burkholderia CZ08152 inoculum suspension. Soak corn seeds of uniform size in 2% NaClO for 30 minutes, wash them with sterile water several times until no NaClO remains, place them in a sterile petri dish at 30°C for 1 day, pour off the excess water, and then place them in a 30 Germinate at ℃. After the seeds are white, they are sown in common straw seedling-raising substrates, and when the second true leaf is fully unfolded, dip the seedling root and transplant it (the specific method is: select seedlings of the same size and place them in a suspension of Burkholderia CZ08152 Among them, as the ex...

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Abstract

The invention discloses a sugarcane endogenous burkholderia sp. CZ08152. The burkholderia sp. CZ08152 is classified and named as burkholderia sp. CZ08152. The 16S rDNA gene sequence table of the strain is as shown in SEQ ID NO.1. The burkholderia sp. CZ08152 is preserved in China General Microbiological Culture Collection Center and the preservation number is CGMCC No. 5743. The sugarcane endogenous burkholderia sp. CZ08152 can be used for producing microbial agents and biological organic fertilizers for promoting crop growing, inhibiting plant diseases and pests and improving anti-stress of plants, thereby improving anti-stress of plants, reducing use of the fertilizer and pesticide, and protecting ecological environment. The sugarcane endogenous burkholderia sp. CZ08152 has a broad application prospect.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a sugarcane endophytic Burkholderia CZ08152 and its application. Background technique [0002] Guangxi is the largest sucrose production area in my country, and the output of sugar cane and sucrose accounts for more than 60% of the country. However, the cost of sugarcane production in my country has always been high and lacks international competitiveness. Among the various agricultural inputs in sugarcane production, chemical fertilizers, especially nitrogen fertilizers, account for the largest proportion and have the most significant impact on sugarcane yield. In addition, more than 90% of the sugarcane land in my country's main sugarcane production areas is dry slope land, the soil water and fertilizer retention capacity is poor, and the utilization rate of chemical fertilizers is very low. Sugarcane tip rot is a common disease of sugarcane. The original fungus is Fusarium monil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 胡春锦曾泉史国英农泽梅岑贞陆叶雪莲
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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