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Extraction method of auricularia auricula polysaccharide

A technique for polysaccharides and extraction methods of fungus, applied in the field of polysaccharide extraction, can solve problems affecting the activity of polysaccharides from fungus, achieve the effects of simple and feasible extraction methods, reduce loss rate, and increase extraction yield

Inactive Publication Date: 2018-06-01
金华市艾力生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This extraction method uses tannic acid solution to remove the protein in the filtrate, which will cause the denaturation of the polysaccharide of the fungus, and then affect the activity of the polysaccharide of the fungus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] The extraction method of fungus polysaccharides includes ultrafine pulverization, ultrasonically enhanced subcritical water extraction, enzymatic hydrolysis-Sevage method for protein removal, and alcohol precipitation. The specific steps are:

[0015] 1) Superfine crushing: wash the fungus, dry, coarsely crush, add grinding aid to the fungus coarse powder at a weight ratio of 1000:0.011-0.013, and superfine crush it to get the fungus superfine powder. A mixture of tolbutamide and voglibose in a weight ratio of 1:0.33-0.38;

[0016] 2) Ultrasound-enhanced subcritical water extraction: add water to the fungus superfine powder according to the ratio of solid to liquid at 1:20-25g / mL, then add 0.03-0.04% of the weight of the fungus powder, dodecanoic acid and 0.010-0.015% of the weight of the fungus powder The extraction time of L-lactic acid is 30-40min under the condition of extraction temperature of 100-110℃, ultrasonic power of 140-150W and pressure of 5-7MPa. Centrifu...

Embodiment 2

[0020] The extraction method of fungus polysaccharides includes ultrafine pulverization, ultrasonically enhanced subcritical water extraction, enzymatic hydrolysis-Sevage method for protein removal, and alcohol precipitation. The specific steps are:

[0021] 1) Superfine crushing: Wash the fungus, dry, coarsely crush, add grinding aid to the fungus coarse powder at a weight ratio of 1000:0.013, and superfine crush it to get the superfine fungus powder, which is used for future use, and the grinding aid is the weight ratio A 1:0.33 mixture of tolbutamide and voglibose;

[0022] 2) Ultrasound-enhanced subcritical water extraction: add water to the fungus superfine powder at a ratio of 1:20 g / mL to liquid, then add 0.04% dodecanoic acid and 0.010% L-lactic acid by weight of fungus powder. Under the conditions of extraction temperature of 110°C, ultrasonic power of 140W, and pressure of 7MPa, the ultrasonically enhanced subcritical water extraction time is 30min. After the extract...

Embodiment 3

[0026] The extraction method of fungus polysaccharides includes ultrafine pulverization, ultrasonically enhanced subcritical water extraction, enzymatic hydrolysis-Sevage method for protein removal, and alcohol precipitation. The specific steps are:

[0027] 1) Superfine crushing: Wash the fungus, dry, coarsely crush, add grinding aid to the fungus coarse powder at a weight ratio of 1000:0.012, and superfine crush it to get superfine fungus powder for use, the grinding aid is the weight ratio A 1:0.35 mixture of tolbutamide and voglibose;

[0028] 2) Ultrasound-enhanced subcritical water extraction: add water to the fungus superfine powder at a material-to-liquid ratio of 1:25g / mL, then add 0.03% dodecanoic acid by weight of the fungus powder and L-lactic acid of 0.015% by weight of the fungus powder. The extraction temperature is 100°C, the ultrasonic power is 150W, and the pressure is 5MPa. The extraction time of ultrasonically enhanced subcritical water is 40min. After the ...

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Abstract

The invention discloses an extraction method of auricularia auricula polysaccharide. The extraction method comprises specific steps as follows: auricularia auricula is subjected to superfine grindingand superfine auricularia auricula powder is obtained; water, dodecanoic acid and L-lactic acid are added to the superfine auricularia auricula powder, ultrasonic reinforced subcritical water extraction is performed, centrifugation is performed after the extraction, and a liquid supernatant is an auricularia auricula polysaccharide extraction liquid; protease is added to the auricularia auricula polysaccharide extraction solution for enzymolysis, centrifugation is performed, the liquid supernatant is subjected to deproteinization with a Sevage method, and a deproteinized polysaccharide liquidis obtained; the deproteinized polysaccharide liquid is concentrated, polysaccharide is precipitated with ethanol, centrifugation, precipitation and freeze-drying are performed, and the auricularia auricula polysaccharide is obtained. The extraction method is simple and feasible, takes a short time, facilitates industrial mass production and has good application value and market potentials, and the auricularia auricula polysaccharide has high extraction rate, purity and antioxidant activity.

Description

technical field [0001] The invention relates to the field of polysaccharide extraction, in particular to a method for extracting polysaccharides from fungus. Background technique [0002] Auricularia auriculajudae (Auricularia auriculajudae), also known as Auricularia auriculajudae, is an edible and medicinal fungus of the fungus Basidiomycetes. Fungus is delicious and nutritious. It is not only an edible fungus with high nutritional value, but also a medicinal fungus with high medicinal value. It is a world-recognized health product. From the perspective of medicinal value, fungus has functions such as delaying aging, assisting in lowering blood fat, anticoagulation, anti-cancer, increasing immunity, assisting in lowering blood sugar, and anti-oxidation. A large number of studies have shown that the physiological functions of edible fungi are closely related to their polysaccharide components. Therefore In recent years, research on polysaccharides has also emerged in an en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 周艳初
Owner 金华市艾力生物科技有限公司
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