Magnetotactic bacterium quantum dot microcapsule and preparation method thereof

A technology of magnetotactic bacteria and microcapsules, which is applied in the preparation of magnetotactic bacteria quantum dot microcapsules and the field of magnetotactic bacteria quantum dot microcapsules, can solve the problems of poor stability, inability to reach, and increase in particle size, etc. Good biocompatibility, good colloidal stability, good dispersion effect

Active Publication Date: 2018-06-01
QUFU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Magnetosome particles are single magnetic domain crystals, uniform in size, nanoscale (20-120nm), and have a large specific surface value. They are coated with biofilm outside the particles, do not produce cytotoxicity, and have excellent biocompatibility. However, due to the high specific surface area of ​​exposed magnetosomes, it is easy to induce aggregation between particles, resulting in increased particle size and poor stability, which cannot meet the requirements of biomedical applications.

Method used

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preparation example Construction

[0038] The present invention also provides a method for preparing the above-mentioned magnetotactic bacterial quantum dot microcapsules, comprising the following steps:

[0039] In step (1), 300 mg of natural graphene was added to a mixture of 60 mL of concentrated sulfuric acid and 20 mL of concentrated nitric acid, and the mixture was first ultrasonically dispersed for 2 h, and then stirred and refluxed at 100° C. for 24 h. The obtained brown-black solution was first cooled to room temperature, and then diluted with deionized water to a total volume of about 800 mL. Sodium carbonate is then added to neutralize the remaining acid until the pH of the solution is around 8. The obtained solution was concentrated by rotary evaporation, and a large amount of precipitated inorganic salts were removed by filtration, and the collected filtrate was dialyzed for four days with a dialysis bag with a cut-off molecular weight of 8000-14000 to obtain an aqueous solution of pure carbon quan...

Embodiment 1

[0053] A preparation method for magnetotactic bacteria quantum dot microcapsules, the steps comprising:

[0054] Step (1) 300mg of natural graphene was added to 60mL of concentrated sulfuric acid and 20mL of concentrated nitric acid mixture, and the mixture was first ultrasonically dispersed for 2h, and then stirred and refluxed at 100°C for 24h. The obtained brown-black solution was first cooled to room temperature, and then diluted with deionized water to a total volume of about 800 mL. Sodium carbonate is then added to neutralize the remaining acid until the pH of the solution is around 8. The obtained solution was concentrated by rotary evaporation, and a large amount of precipitated inorganic salts were removed by filtration, and the collected filtrate was dialyzed for four days with a dialysis bag with a cut-off molecular weight of 8000-14000 to obtain an aqueous solution of pure carbon quantum dots.

[0055] In the above step (1), the aqueous solution of carbon quantum...

Embodiment 2

[0061] A preparation method for magnetotactic bacteria quantum dot microcapsules, the steps comprising:

[0062] Step (1) 300mg of natural graphene was added to 60mL of concentrated sulfuric acid and 20mL of concentrated nitric acid mixture, and the mixture was first ultrasonically dispersed for 2h, and then stirred and refluxed at 100°C for 24h. The obtained brown-black solution was first cooled to room temperature, and then diluted with deionized water to a total volume of about 800 mL. Sodium carbonate is then added to neutralize the remaining acid until the pH of the solution is around 8. The obtained solution was concentrated by rotary evaporation, and a large amount of precipitated inorganic salts were removed by filtration, and the collected filtrate was dialyzed for four days with a dialysis bag with a cut-off molecular weight of 8000-14000 to obtain an aqueous solution of pure carbon quantum dots. Freeze and drain the aqueous solution of carbon quantum dots to obtain...

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Abstract

The invention provides a magnetotactic bacterium quantum dot microcapsule. The inner core of the microcapsule is a magnetosome, the surface of the magnetosome is coated with a uniform and compact compound of chitosan oligosaccharide / gamma-polyglutamic acid / carbon quantum dots, and the mass ratio of the magnetosome to chitosan oligosaccharide to gamma-polyglutamic acid to carbon quantum dots is 1:(1-3):(10-20):1. The preparation method of the magnetotactic bacterium quantum dot microcapsule is easy to operate, the obtained magnetosome and carbon quantum dot intelligent microcapsule represent typical core-shell structures, and the quantum dot microcapsule has the targeting and slow releasing capability, and has huge clinical application prospects.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a magnetotactic bacterial quantum dot microcapsule, and a preparation method for the magnetotactic bacterial quantum dot microcapsule. Background technique [0002] The way of drug delivery has a crucial impact on the therapeutic effect of cancer, and tumor-targeted drug delivery is very challenging. With the deepening of nanotechnology research in the field of biomedicine, magnetic nanomaterials have also received a lot of attention. Magnetotactic bacteria (Magnetotactic bacteria) is a kind of bacteria that can perform directional movement under the action of an external magnetic field and form nano-magnetic particles—Magnetosomes (Magnetosomes) in the body. In recent years, magnetotactic bacteria and magnetosomes have become new The biological resources are widely studied in materials science, medicine, biology, physics, geology and other disciplines, and have been applied in bionic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/46A61K47/36A61K47/34A61K47/04A61K9/00A61K31/704A61K41/00A61P35/00
CPCA61K9/0009A61K9/501A61K9/5031A61K9/5036A61K31/704A61K41/0052A61K47/46A61K2300/00
Inventor 杨革李媛车程川巩志金梁鑫鑫
Owner QUFU NORMAL UNIV
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