Vascular dementia model of influence of hemopoietin on choline acetyl transferase

Abstract

The invention belongs to the technical field of bioassay, and discloses a vascular dementia model of the influence of hemopoietin on choline acetyl transferase. A big mouse not showing the symptom ofthe lesion of the vascular dementia model is subjected to water deprivation and is allowed to continuously and naturally drink a 1%-4% methyl alcohol aqueous solution for 60-240 days, or banana tasteflavouring agent capable of making the big mouse generate preference is further dissolved in the methyl alcohol aqueous solution, the methyl alcohol intake of the big mouse per day is controlled at 1mg / kg to 2 mg / kg, and the vascular dementia model capable of showing the influence of hemopoietin on choline acetyl transferase can be obtained; after the vascular dementia model the influence of hemopoietin on choline acetyl transferase is obtained, the dementia type disorder and the like are evaluated. The acting mechanism of ChAT in EPO for improving the VaD big mouse recognition disorder isdetermined.

Description

technical field [0001] The invention belongs to the technical field of biological testing, in particular to a vascular dementia model of the influence of erythropoietin on choline acetyltransferase. Background technique [0002] Currently, vascular dementia (VaD) is an important cause of senile dementia. Studies have shown that in addition to pathological changes in brain structure during the occurrence of dementia, the metabolism of neurotransmitters and enzymes (such as acetylcholine (Ach) and choline acetyltransferase (Ch AT) related to the process of learning and memory in the brain are also affected. An exception occurs. ChAT is considered to be an enzyme that promotes the metabolism of the neurotransmitter ACh in the brain related to learning and memory, and its content in memory-related brain regions is positively correlated with learning and memory abilities. In recent years, foreign studies have found that the secretion of erythropoietin (EPO) in the central nervo...

AI Technical Summary

Problems solved by technology

Cons: Recoverable, not permanent damage

Disadvantages: Although this experimental model is very commonly used and effectively simulates its pathological characteristics, it is still different from the characteristics in the natural state, causing certain physical damage to the animal, and it is difficult to grasp the cut site, which may cause other Nerve cells in the site can also cause damage, which has high requirements on the technique of the experimenter, and it is basically not used at present.

The disadvantage is that most of the transgenic animal models can replicate some of the pathological changes in the brain of AD patients, and only a few of them have observed learning and memory dysfunction, but there is still a certain distance from AD, and the results are not satisfactory.

[0013] To sum up, the problem in the prior art is that the mechanism of action of ChAT in EPO improving the cognitive impairment of VaD rats is not clear

There is no method to establish a relevant and effective animal model of senile dementia; and the existing drugs for the treatment of senile dementia have poor efficacy

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