Preparation method of oral TGEV (transmissible gastroenteritis virus) deactivating microcapsule

Abstract

The invention provides a preparation method of an oral TGEV (transmissible gastroenteritis virus) deactivating microcapsule, and relates to a preparation method of an oral TGEV microcapsule, which aims at overcoming the technical defects of oral deactivating vaccine in the existing domestic preventing and controlling phase of TGEV. The preparation method of the oral TGEV microcapsule is based on the TGEV deactivating and oral microcapsule preparation techniques. Proofed by animal experiment results, the preparation method has the advantages that the titer detection result of serum neutralizingantibody indicates that the microcapsule vaccine can induce the response of mucosal immune and system immune of a piglet and produce higher level of serum and mucosal antibodies, and objectively indicates that the microcapsule vaccine has certain slow-release effect; after the antigen matter of the microcapsule vaccine is orally taken by the piglet, the expression of specific antibodies IgG and IgA of the piglet can be induced, the resistance ability of the piglet to TGEV is improved, the death rate of the piglet is reduced, and the antivirus protection rate is 60 to 75% higher than the antivirus protection rate of a control group.

Description

technical field [0001] The invention relates to the inactivation technology of porcine transmissible gastroenteritis virus and the preparation technology and method of microcapsules. Background technique [0002] Infection with porcine transmissible gastroenteritis virus (TGEV) is one of the main causes of porcine viral diarrhea (TGEV), which is characterized by severe enteritis, vomiting, watery diarrhea, and weight loss. The disease occurs throughout the year, especially in winter, and pigs of all ages are susceptible to infection, but it is the most harmful to piglets and has the highest case fatality rate. Severe piglet mortality makes TGEV infection a major factor in the loss of China's pig industry. The life cycle of a coronavirus begins by invading living cells. After the adsorption is completed, the virus needs to enter the cytoplasm of the host cell in the next step. The virus can insert into the cell membrane and fuse with the membrane of the helper cell, thereb...

AI Technical Summary

Problems solved by technology

However, there is still little research on the prevention and control and application of animal diseases, especially in the prevention and control of pig diseases, only microcapsules are used to encapsulate the TGEV neutralizing antigen COE protein expressed by E. coli prokaryotic

Does not use microencapsulated preparations to encapsulate inactivated microorganisms, including porcine inactivated pathogenic bacteria and viruses

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