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Screening method for piglet intestinal-function protection agent

A technology of intestinal function and screening method, applied in the direction of material testing products, test food, etc., can solve the problem of poor pertinence, limited screening methods, intestinal damage model, intestinal function measurement index piglet age, and test period inconsistency and other problems, to achieve the effect of strong pertinence, high repeatability and accurate results

Inactive Publication Date: 2018-05-18
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional feed antibiotics will be gradually banned due to drug resistance and residual problems. Therefore, in order to improve piglet diarrhea, it is necessary to screen out feed additives that can protect pig intestinal function.
[0003] However, in the prior art, there are very limited screening methods for feed additives with protective effects on pig intestinal function, and the adopted intestinal injury models, intestinal function measurement indicators, piglet ages, test periods, etc. are not consistent, easily As a result, the repeatability of the test results is poor, the application effect of the screened feed additives is unstable, and the pertinence is not strong, and it is impossible to accurately and quickly screen out piglet intestinal function protection additives suitable for piglet feeding

Method used

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  • Screening method for piglet intestinal-function protection agent
  • Screening method for piglet intestinal-function protection agent
  • Screening method for piglet intestinal-function protection agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] (1) Select 40 healthy and good-bred piglets to be weaned at the age of 7 days, fed with high-quality artificial milk, and put the 7-day-old piglets in metabolic cages for 3 days, and then put the test piglets (10 days old, the first experiment) days) were randomly divided into four groups (10 piglets in each group) according to the principle of similar body weight: blank control group (Group Ⅰ), indomethacin group (Group Ⅱ), test group (Group Ⅲ), test control group (Group IV Group). Among them, group III used N-acetylcysteine ​​(NAC) as an additive to be screened for oral gavage, and the dosage range was controlled at 40 mg per kilogram of body weight, once a day for 6 consecutive days; group IV used alanine (Ala) Orally administered as the additive to be screened, the dosage range was controlled at 40 mg per kilogram of body weight, once a day, for 6 consecutive days; Group I and Group II were orally administered the same volume of normal saline for 6 consecutive days....

Embodiment 2

[0082] Embodiment 2 Test piglet growth performance

[0083]Table 1 shows the calculation results of average daily gain and feed-to-weight ratio of test piglets in Group I, Group II, Group III and Group IV.

[0084] Growth performance of each group of test piglets in Table 1 Example 2

[0085] project

[0086] It can be seen from Table 1 that compared with group Ⅰ, the average daily gain of piglets in group Ⅱ decreased by 30.23% (P<0.05), but the feed-to-weight ratio increased by 31.65% (P<0.05); Compared with group Ⅲ, NAC treatment can increase the average daily gain of piglets by 26.79%, and reduce the feed-to-weight ratio by 19.23%. It shows that the additive NAC to be screened can alleviate the adverse effects of indomethacin stimulation on piglet growth performance, while the additive Ala to be screened has no obvious help in alleviating the adverse effects of indomethacin stimulation on piglet growth performance.

Embodiment 3

[0087] Example 3 Histopathological changes in the intestinal tract of test piglets

[0088] (1) When the test piglets were slaughtered and dissected, observe and take photos to record the appearance of the intestinal tissue of the test piglets in Group I, Group II, Group III and Group IV. The results are as follows: Figure 1 to Figure 5 shown. in, figure 1 , figure 2 , Figure 4 , Figure 5 The appearance and morphology of intestinal tissues of piglets in groups I, II, III and IV are shown in sequence, image 3 for figure 2 Appearance diagram of the ileum after section A in the middle part.

[0089] Depend on Figure 1 to Figure 4 It can be seen from the observation that in groups Ⅱ and 4, when the piglets in the test were fed with normal saline and then injected with indomethacin, a large area of ​​bleeding point appeared in the rear part of the intestinal tract (jejunum and ileum) ( figure 2 As shown in A in the middle), after dissecting the ileum, it was found t...

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Abstract

The invention discloses a screening method for a piglet intestinal-function protection agent. The screening method includes the following steps that test piglets are divided into I piglets, II pigletsand III piglets, oral cavities of the I piglets and oral cavities of the II piglets are drenched with normal saline, and oral cavities of the III piglets are drenched with a to-be-screened additive;from the seventh day of testing, at the time 1 h after the oral cavities of the I piglets and the oral cavities of the II piglets are drenched with the normal saline, and the oral cavities of the IIIpiglets are drenched with the to-be-screened additive, the I piglets are subjected to normal saline intraperitoneal injection, and the II piglets and the III piglets are subjected to indometacin intraperitoneal injection; on the tenth day of testing, the I piglets, the II piglets and the III piglets are subjected to D-xylose oral cavity drenching at the same time, blood samples of the test pigletsare collected after 1 h, and blood indexes are measured; at the time 2 hours after the blood samples are collected, small intestine samples of the test piglets are taken, intestinal pathologic changes are observed, and the mRNA level of intestinal-function genes is detected. The screening method for the piglet intestinal-function protection agent has the advantages of being short in test cycle, high in pertinence, accurate in result and high in repeatability.

Description

technical field [0001] The invention relates to the technical field of piglet feed additives, in particular to a screening method for a piglet intestinal function protective agent. Background technique [0002] The intestine is not only the final place for digestion and absorption of all nutrients, but also the largest immune organ in the animal body and the first barrier of the body's defense system. Maintaining the health of the animal intestine is crucial to ensuring pig production. Studies have proved that the intestine is the central organ of the body's stress response, and various stress factors (such as psychological, physiological, nutritional and pathogenic stress factors) can cause intestinal dysfunction and damage the structure of the piglet intestine And function, cause diarrhea in piglets, causing serious economic losses. Traditional feed antibiotics will be gradually banned due to drug resistance and residual problems. Therefore, in order to improve piglet dia...

Claims

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Application Information

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IPC IPC(8): G01N33/02
CPCG01N33/02
Inventor 侯永清易丹丁斌鹰吴涛
Owner WUHAN POLYTECHNIC UNIVERSITY
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