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Application of micro-molecule polypeptide KP-1 in preparing medicine for treating chronic kidney diseases

A technology for small molecule peptides and chronic kidney disease, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc.

Active Publication Date: 2018-05-18
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still no effective drugs to delay the progression of CKD clinically.

Method used

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  • Application of micro-molecule polypeptide KP-1 in preparing medicine for treating chronic kidney diseases
  • Application of micro-molecule polypeptide KP-1 in preparing medicine for treating chronic kidney diseases
  • Application of micro-molecule polypeptide KP-1 in preparing medicine for treating chronic kidney diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Screening of KP-1 Small Molecule Peptides

[0025] 1. Experimental materials:

[0026] Cells: Rat kidney fibroblasts (NRK-49F).

[0027] Culture medium: DMEM / F12 (1:1) culture medium containing 10% FBS.

[0028] Culture conditions: 37 o C contains 5% CO 2 incubator.

[0029] Small molecule peptide library: 18 peptide sequences were designed by computer, each with an average length of 30 amino acids, which were artificially synthesized by Nanjing GenScript Biotechnology Co., Ltd. to build a small molecule peptide library.

[0030] 2. Experimental treatment:

[0031] (I) The cultured rat kidney fibroblasts were mixed with 1.5×10 6 They were planted in 6-well cell culture plates, cultured for 1 day, starved for 24 hours, and given 18 different small molecule peptides (10 µg / ml) 1 hour in advance. Then, TGF-β recombinant protein (2 ng / ml) was given to stimulate, and the protein was harvested 48 hours later, and Western blot experiments were performed to sc...

Embodiment 2

[0039] Example 2: Distribution of FITC-labeled KP-1 in various organs in UUO mice

[0040] 1. Experimental animals: C57 mice, male, weighing 20-22g, SPF grade.

[0041] The animals were first weighed and numbered, and 2 healthy mice weighing 20-22 g were selected and divided into UUO and UUO+KP-1.

[0042] 2. Experimental grouping

[0043] 1) UUO mice: at room temperature, anesthetize the mouse with 1% pentobarbital sodium at 5ml / kg body weight, select the left abdomen 2-3cm as the incision; after local disinfection, cut the skin, subcutaneous, muscle layer and For the peritoneum, after finding the left ureter, ligate the upper 1 / 3 of the ureter and suture layer by layer. After local disinfection, check the marks and place them in corresponding mouse cages.

[0044] 2) UUO+KP-1 mice: anesthetize and disinfect as above. The skin, subcutaneous layer, muscle layer and peritoneum were cut layer by layer. After finding the left ureter, the upper 1 / 3 segment of the ureter was li...

Embodiment 3

[0049] Example 3: Inhibitory effect of small molecule polypeptide KP-1 on renal fibrosis in mouse UUO model

[0050] 1. Experimental animals: BALB / C mice, male, weighing 20-22g, SPF grade.

[0051] The animals were first weighed and numbered, and 12 healthy mice weighing 20-22 g were selected and randomly divided into 3 groups, 6 in each group. Including sham operation group, model control group and medication group.

[0052] 2. Experimental grouping

[0053] 1) Sham operation group: After mice were anesthetized with 1% sodium pentobarbital at 5 ml / kg body weight at room temperature, the incision was 2-3 cm from the left abdomen; after local disinfection, the skin, subcutaneous layer, and muscular layer were cut layer by layer And peritoneum, found the left ureter, then sutured layer by layer. After local disinfection, check the marks and place them in corresponding mouse cages.

[0054] 2) Model control group: anesthetized and disinfected as above. The skin, subcutaneous...

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Abstract

The invention relates to a novel application of a micro-molecule polypeptide KP-1, and in particular to an application of the micro-molecule polypeptide KP-1 in preparing a medicine for treating chronic kidney diseases (CKD). The invention provides the application of the KP-1 in treating the CKD; and the KP-1 is obvious in curative effect and is free from obvious toxic and side effects. Therefore,the KP-1 provided by the invention can be used for preparing a pharmaceutical preparation for treating the CKD.

Description

technical field [0001] The present invention relates to the use of a unique small molecule polypeptide in the treatment of chronic kidney disease (CKD), in particular to the use of KP-1 polypeptide in the preparation of medicines for treating CKD. Background technique [0002] Chronic kidney disease (CKD) eventually progresses to end-stage renal failure (ESRD), and patients rely on "renal replacement therapy" for life to maintain their lives. In the past few decades, along with the aging of the human society, the prevalence of CKD has been increasing year by year (Nat Rev Nephrol, 2011, 7: 684-696). Some data indicate that CKD is becoming a "public health problem", which seriously endangers human health and consumes a lot of health resources. However, currently there are no drugs that can effectively delay the progression of CKD clinically. Aiming at the pathogenesis of CKD, finding drugs that can effectively inhibit or delay the progress of CKD is undoubtedly an urgent ta...

Claims

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Application Information

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IPC IPC(8): A61K38/16A61P13/12
CPCA61K38/16
Inventor 周丽丽
Owner NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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