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A kind of pharmaceutical composition containing carbamazepine and biotin and application thereof

A composition and biotin technology, applied in the field of medicine, to achieve the effect of reducing mortality, good safety and significant therapeutic effect

Active Publication Date: 2019-11-15
武汉百药联科科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no precedent for the combination of carbamazepine and biotin in the treatment of flavivirus infections

Method used

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  • A kind of pharmaceutical composition containing carbamazepine and biotin and application thereof
  • A kind of pharmaceutical composition containing carbamazepine and biotin and application thereof
  • A kind of pharmaceutical composition containing carbamazepine and biotin and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Application of carbamazepine combined with biotin in treating mice infected with Japanese encephalitis virus

[0037] 1. Establishment of a mouse model infected with Japanese encephalitis virus

[0038] 1. Experimental materials:

[0039] (1) Experimental animals: four-week-old Balb / c female mice.

[0040] (2) Strain: JEV P3 strain.

[0041] (3) Other reagents:

[0042] 1) 0.01M PBS buffer: weigh NaH 2 PO4 0.593g, Na 2 HPO4 5.802g, NaCl17.0g, deionized water (ddH 2 O) Dilute to 2L, store at room temperature for later use.

[0043] 2) DMEM basal medium: a bottle of DMEM powder, weighed NaHCO 3 3.7g, HEPES5.95g, add 800mLddH 2 O, stir well to dissolve, dilute to 1000mL, sterilize with a 0.22μm filter, and store at 4°C for later use.

[0044] 2. Experimental steps

[0045] Mice were injected intraperitoneally with 10 6 PFU Japanese encephalitis virus, the mice in the control group were intraperitoneally injected with the same dose of DMEM medium. In ...

Embodiment 2

[0064] Example 2 Application of biotin in treating mice infected with Japanese encephalitis virus

[0065] 1. Establishment of a mouse model infected with Japanese encephalitis virus

[0066] Step is with embodiment 1

[0067] 2. Evaluation of the therapeutic effect of biotin on mice infected with Japanese encephalitis virus

[0068] 1. Experimental drugs

[0069] Biotin, traits: white powder, usage: 5% DMSO+30% PEG+ddH 2 O dissolved and administered orally.

[0070] 2. Specific experimental steps

[0071] The content of this part is consistent with the experimental procedure in Example 1, and the oral dose of biotin is 3 mg / kg. Observe and record the number of dead mice.

[0072] 3. Experimental results

[0073] 1. The effect of biotin on the survival rate of JEV-infected mice.

[0074] The challenge experiment lasted until 23 days after infection, and the surviving mice basically had no obvious neurological symptoms. In the 5-10 days of infection, the number of deat...

Embodiment 3

[0078] Example 3 Drug efficacy prediction experiment based on flavivirus infection personalized drug method

[0079] (1) Obtain the gene expression data of Japanese encephalitis virus-infected patients, West Nile virus-infected patients and healthy people.

[0080] The chip data (series: GSE57647, GSE43190) of Japanese encephalitis virus patients, West Nile virus infected patients and control group were downloaded from NCBI GEO Dataset, and a total of 6 Japanese encephalitis virus infected patients and 18 infected patients were obtained. Gene expression data from West Nile virus patients.

[0081] (2) Calculating the key gene list of patients with flavivirus infection

[0082] Based on the gene names in the 24 chip data downloaded in (1) and the gene interaction information downloaded from the STRING database, construct a gene interaction matrix, and calculate the multiple difference value of each gene in each patient as the initial importance input into the GeneRank algorith...

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Abstract

The invention discloses a medicine composition containing carbamazepine and biotin and application thereof, and concretely relates to application of the medicine composition containing the biotin andthe carbamazepine in preparing Japanese B encephalitis virus infection medicines, zika virus infection medicines, west nile virus infection medicines, yellow fever virus infection medicines and 1-4 type dengue virus infection medicines. According to the medicine composition provided by the invention, the death rate of rats infected by Japanese B encephalitis virus can be effectively reduced; in addition, the biotin is vitamin necessary for a human body, and the carbamazepine is applied clinically for a long time and has favorable safety, so that the medicine composition containing the biotin and the carbamazepine can be further developed to be a medicine for treating flavivirus infection, and provides a new choice for clinical medication.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a pharmaceutical composition containing carbamazepine and biotin and its application. Background technique [0002] The Flavivirus family (Flaviviridae) is an enveloped single-stranded positive-sense RNA virus, which is transmitted by blood-sucking arthropods (mosquitoes, ticks, sandflies, etc.) to cause infection. The flavivirus genus includes about 70 viruses, most of which are pathogens of zoonotic infectious diseases, such as Japanese encephalitis virus (Japanese encephalitis virus, JEV), West Nile virus (WNV), yellow fever Virus (Yellowfevervirus, YFV), Dengue virus (DENV) type 1-4, Zika virus (Zika virus, ZIKV), etc. [0003] The gene of the flavivirus genus is a single-stranded positive-strand RNA, about 11kb in length, with a non-coding region (UTR) at the 5' end and a 3' end, a methylated cap structure at the 5' end, and a methylated cap structure at the 3' end. Does ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/55A61K31/4188A61P31/14
CPCA61K31/4188A61K31/55A61K2300/00Y02A50/30
Inventor 张红雨吕博敏全源张青叶黄清
Owner 武汉百药联科科技有限公司
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