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Auricularia auricula-judae wall-breaking method

A technology of black fungus and bacillus, applied in the field of black fungus wall breaking, can solve the problems of low absorption and utilization rate, and achieve the effects of low cost, less equipment investment and stable process

Active Publication Date: 2018-05-08
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to overcome the strong cell wall of black fungus fruit bodies and the low absorption and utilization rate after eating, the purpose of the present invention is to provide a strain of Bacillus siamese with the ability to secrete chitinase and its application in the aspect of black fungus wall breaking, thereby Promote the full dissolution of nutrients in the fruiting bodies of black fungus, and greatly improve the bioavailability of black fungus

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0032] 1. Primary screening of strains

[0033] Collect 10 fresh feces samples of healthy piglets from all over Jilin Province, weigh 10g samples respectively, add 90mL sterile saline to prepare a sample suspension, and take 100μL 10 -2 、10 -3 、10 -4 The diluted solution was spread on the LB medium plate, and each gradient was spread in three parallels, and cultured at a constant temperature of 37°C for 48 hours, and then the bacillus strains were purified by plate streaking method, and were numbered and stored separately. 122 strains of Bacillus were obtained.

[0034] 2. Preliminary screening of chitinase-producing bacillus

[0035] Spot the isolated strains on the above-mentioned separation plate with chitin powder as the only carbon source, and incubate at 37°C for 16-48 hours. If a transparent hydrolysis circle appears around the grown colony, it means that the chitin in the medium has been It is hydrolyzed by the enzyme secreted by the strain, which proves that the s...

Embodiment 2

[0055] The preparation of embodiment 2 bacillus siamese JAASHD broken wall black fungus powder

[0056] Preparation of crude enzyme solution:

[0057] Inoculate the strain JAASHD in LB liquid fermentation medium, place on a shaker at 35°C, 240rpm, shake to OD 600 The value 0.8 is taken out. Centrifuge at 6000rpm for 20min, discard the precipitate, and filter the supernatant at 0.22um to obtain the crude enzyme solution.

[0058] The composition of the LB liquid fermentation medium is: peptone 12g / L, yeast powder 2g / L, sodium chloride 4g / L, glucose 2g / L, deionized water 1000mL; pH=6.2. Autoclaved at 121°C for 20min.

[0059] Breaking the wall of black fungus fruiting bodies:

[0060] The black fungus powder is added into the crude enzyme liquid at 10% (w / v), shaken gently, statically hydrolyzed at 36° C. to 38° C. for 24 hours, and then dried to obtain the broken black fungus powder.

Embodiment 3

[0061] The preparation of embodiment 3 bacillus siamese JAASHD broken wall black fungus powder

[0062] Preparation of crude enzyme solution:

[0063] Inoculate the strain JAASHD in LB liquid fermentation medium, place on a shaker at 42°C, 150rpm, shake to OD 600 A value of 1.0 is taken out. Centrifuge at 10,000rpm for 15min, discard the precipitate, and filter the supernatant at 0.22um to obtain the crude enzyme solution.

[0064] The composition of the LB liquid fermentation medium is: peptone 8g / L, yeast powder 4g / L, sodium chloride 6g / L, glucose 4g / L, deionized water 1000mL; pH=7.0. Autoclaved at 121°C for 20min.

[0065] Breaking the wall of black fungus fruiting bodies:

[0066] The black fungus powder is added into the crude enzyme solution at a rate of 30% (w / v), shaken gently, statically hydrolyzed at 36° C. to 38° C. for 48 hours, and then dried to obtain the broken black fungus powder.

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Abstract

The invention relates to the field of processing of microbes and agricultural products and in particular relates to an auricularia auricula-judae wall-breaking method. The method comprises the following steps: co-incubating auricularia auricula-judae and bacillus siamensis enzyme solution, and performing enzymolysis and wall breakage; the bacillus siamensis strain is collected in China General Microbiological Culture Collection Center, has a collection number of CGMCC NO.12903 and a collection date of August 24, 2016. According to the method disclosed by the invention, the auricularia auricula-judae fruiting body is wall-broken by utilizing the screened bacillus siamensis, so that polysaccharides and other active ingredients of are promoted to be dissolved out of cells, and the biologicalabsorption utilization rate of the auricularia auricula-judae is improved.

Description

technical field [0001] The invention relates to the field of microorganism and agricultural product processing, in particular to a method for breaking the wall of black fungus. Background technique [0002] Black fungus (Auricularia auricula-judae), also known as fungus, ear, and fungus, belongs to the fungus Basidiomycetes and is extremely nutritious. It is known as "human body scavenger" and "food aspirin". It is a precious medicinal and edible colloid fungus in my country. It is produced in most areas of China, and its annual output accounts for more than 90% of the world's total output. [0003] Modern medicine has confirmed that black fungus has anti-coagulation, anti-tumor, anti-inflammation and other cell protection effects, and also has various physiological functions such as lowering blood lipids, blood sugar, blood viscosity, cholesterol, anti-diabetes, anti-aging, anti-radiation, and anti-fatigue At the same time, it can also promote the biosynthesis of nucleic a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12R1/645
CPCC12N1/06
Inventor 王景会李达王颖牛红红苗欣宇迟燕平刘香英康立宁李玉秋姜媛媛
Owner JILIN ACAD OF AGRI SCI
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