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A kind of surface-modified acellular matrix and its modification method

An acellular matrix and surface modification technology, which is applied in the field of surface-modified acellular matrix and its modification, can solve the problems of accelerating the degradation of acellular matrix, inhibit cell infiltration, resist long-term erosion, and increase speed and quantity Effect

Active Publication Date: 2020-09-25
THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] 3. Tumor cells mainly obtain energy through glycolysis, and metabolism will produce a large amount of lactic acid, making the surrounding environment weakly acidic, thus accelerating the degradation of acellular matrix
[0012] In conclusion, the existing modification methods of acellular matrix materials have solved many problems in clinical application, but facing the physiological environment after malignant tumor surgery, it is necessary to solve the problem of anti-degradation of acellular matrix materials under the action of tumor cells, inflammatory cells, and lactic acid. problem, thus requiring the design of new acellular matrix modification methods

Method used

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  • A kind of surface-modified acellular matrix and its modification method
  • A kind of surface-modified acellular matrix and its modification method
  • A kind of surface-modified acellular matrix and its modification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The modified decellularized pericardium of this embodiment is prepared by the following method:

[0064] (1) Carboxyl activation of decellularized pericardium

[0065] Take a 10 cm×5 cm decellularized pericardium (DPP), soak it in 8 mL of 2-(N-morpholine)ethanesulfonate (MES) solution (pH=6.5), add 0.85 g of EDC, 0.51 g of NHS, and stir for 12 h, Activation of carboxyl groups on the surface of decellularized pericardium. Dialyze in ultra-pure water (dialysis bag molecular weight cut-off 500 Da) for 24 h to remove the activator and set aside.

[0066] (2) Surface modification of decellularized pericardium

[0067] Add 0.3 g of L-phenylalanine benzyl ester hydrochloride containing carboxyl-protecting groups to 10 mL of MES solution (pH=6.5), 0.57 g of tetrabutylammonium bromide, and add carboxyl-activated decellularized The pericardium was stirred for 48 h to make the amino group of phenylalanine graft react with the carboxyl group on the surface of the acellular matri...

Embodiment 2

[0090] The modified decellularized amniotic membrane of this example is prepared by the following method:

[0091] (1) Carboxyl activation of decellularized amniotic membrane

[0092] Take 5 cm×5 cm decellularized amniotic membrane, immerse in 10 mL 2-(N-morpholine)ethanesulfonate (MES) solution (pH=6.5), add 0.57 g EDC, stir for 12 h, activate surface carboxyl groups, and set aside.

[0093] (2) Surface modification of decellularized amnion

[0094] The carboxyl group of phenylalanine was protected by esterification, and the reference for the preparation method (Zeng Guangxiang, Lu Huibang, Sun Shengling, et al. Synthesis and antibacterial activity of phenylalanine ethyl sorbate[J]. Food Industry Science and Technology, 2013 , 34(24):321-325.), to obtain L-phenylalanine ethyl ester.

[0095] Add 0.5 g of L-phenylalanine ethyl ester to 10 mL of the solution in step 1, and stir in an ice bath for 12 h to allow the amino group of phenylalanine to react with the carboxyl group ...

Embodiment 3

[0112] The modified decellularized peritoneum of this example was prepared by the following method:

[0113] (1) Carboxyl activation of decellularized peritoneum

[0114] Take a 10 cm×10 cm decellularized peritoneum, immerse it in 8 mL 2-(N-morpholine)ethanesulfonate (MES) solution (pH=5.8), add 1.51 g NHS, and stir for 12 h to activate the carboxyl groups of the decellularized peritoneum . After dialysis in ultrapure water (dialysis bag molecular weight cut-off 500 Da) for 6 h, set aside.

[0115] (2) Surface modification of decellularized peritoneum

[0116] To 20 mL of 70% dichloromethane MES solution (pH=6.5), add 0.8 g of L-phenylalanine benzyl ester hydrochloride containing a carboxyl protecting group, 1 g of tetrabutylammonium bromide, dissolve completely and add The decellularized peritoneum after carboxyl activation was sonicated for 2 h (37 Hz, 580 W) to allow the amino group of phenylalanine to react with the carboxyl group on the surface of the decellularized pe...

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Abstract

The invention belongs to the field of tissue engineering, and particularly relates to a surface modifying method of an acellular matrix. The method includes the following steps of firstly, conductingcarboxyl activating on the acellular matrix; secondly, conducting carboxyl protecting on phenylalanine or phenylalanine derivatives, and conducting grafting modifying on the carboxyl-protecting phenylalanine or phenylalanine derivatives through amino of the phenylalanine or phenylalanine derivatives and carboxyl of the acellular matrix; thirdly, conducting postprocessing on the grafting-modified acellular matrix to obtain the surface-modified acellular matrix. The improved acellular matrix has functions of resisting tumor cell and inflammatory cell infiltration, resisting infection, resistingmatrix metal protease and lactic acid degradation and improving the inflammation environment and can be used for postoperative tissue repairing of tumor treatment.

Description

technical field [0001] The invention relates to the field of tissue engineering, in particular to a surface-modified acellular matrix and a modification method thereof. Background technique [0002] As a natural biodegradable material, acellular matrix material has rich bioactive components, natural three-dimensional network structure, and low immunogenicity, and has been widely used in the field of tissue engineering. In terms of tumor treatment, acellular matrix materials have been used to solve the problem of mucosal injury repair after throat tumor resection, (Zhang Qingquan, Sun Yan, Wang Qiang, et al. Heterogeneous (bovine) acellular dermal matrix repaired throat tumor resection mucosa Effect evaluation of defect [J]. Chinese Tissue Engineering Research, 2008, 12(06):1081-1084.), there is an urgent need for the preparation technology of acellular matrix materials to solve the problem of postoperative repair of malignant tumors. [0003] The special physiological envir...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/54A61L27/58
CPCA61L27/3604A61L27/3687A61L27/3691A61L27/54A61L27/58A61L2300/404A61L2300/406A61L2300/41A61L2300/416A61L2300/602A61L2430/40
Inventor 沈靖南尹军强武征林熙
Owner THE FIRST AFFILIATED HOSPITAL OF SUN YAT SEN UNIV
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