3D printing preparation method of skin tissue engineering scaffold and in vitro cell toxicity testing method of the same

A technology for skin tissue engineering and tissue engineering scaffolds, applied in the field of preparation of high-strength materials, can solve problems such as poor biocompatibility, poor mechanical properties, and fast degradation speed, and achieve good mechanical properties, simple operation, and good air permeability. and absorbent effect

Inactive Publication Date: 2018-05-08
YANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic materials have good mechanical properties and plasticity, and are easy to print, but their degradation products often cause surrounding inflammation and poor biocompatibility
Natural polymers have good biocompatibility, biodegradability and biological activity, which can better induce the self-recovery of injured skin, but the mechanical properties are poor, and the degradation rate is too fast under the action of various enzymes in the body , the stent has collapsed before the new tissue grows well

Method used

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  • 3D printing preparation method of skin tissue engineering scaffold and in vitro cell toxicity testing method of the same
  • 3D printing preparation method of skin tissue engineering scaffold and in vitro cell toxicity testing method of the same
  • 3D printing preparation method of skin tissue engineering scaffold and in vitro cell toxicity testing method of the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Preparation of dialdehyde-based nanocellulose (DAC): The mass ratio of CNC to sodium periodate is 1:1, react in the dark at 40°C for 6 hours, and then centrifuge and dialyze until the free ions in the solution are completely removed ;

[0033] (2) Preparation of gelatin solution: add gelatin to phosphate buffered solution (PBS), and keep stirring until the gelatin is completely dissolved;

[0034] (3) Preparation of dialdehyde-based nanocellulose (DAC) / gelatin (GEL) composite hydrogel: blend 4% DAC solution and 4% GEL solution at a volume ratio of 1:1, rapidly After stirring evenly, incubate at 37°C for 2 hours;

[0035] (4) Printing of 3D tissue engineering scaffolds: DAC / GEL hydrogel is loaded into the barrel, the barrel temperature is 10°C, the platform temperature is 5°C, the needle diameter is 200μm, the extrusion pressure is 0.1Mpa, and the nozzle travel speed is 15mm / S, complete the 3D printing of the tissue engineering scaffold.

Embodiment 2

[0037] (1) Preparation of dialdehyde-based nanocellulose (DAC): The mass ratio of CNC to sodium periodate is 1:2, react in the dark at 40°C for 4 hours, and then centrifuge and dialyze until the free ions in the solution are completely removed ;

[0038] (2) Preparation of gelatin solution: add gelatin to phosphate buffered solution (PBS), and keep stirring until the gelatin is completely dissolved;

[0039] (3) Preparation of dialdehyde-based nanocellulose (DAC) / gelatin (GEL) composite hydrogel: blend a 5% DAC solution with a 5% GEL solution at a volume ratio of 4:6, rapidly After stirring evenly, incubate at 37°C for 3 hours;

[0040](4) Printing of 3D tissue engineering scaffolds: DAC / GEL hydrogel is loaded into the barrel, the barrel temperature is 8°C, the platform temperature is 8°C, the needle diameter is 220μm, the extrusion pressure is 0.15Mpa, and the nozzle travel speed is 20mm / S, complete the 3D printing of the tissue engineering scaffold.

Embodiment 3

[0042] (1) Preparation of dialdehyde-based nanocellulose (DAC): The mass ratio of CNC to sodium periodate is 1:2, react in the dark at 40°C for 5 hours, and then centrifuge and dialyze until the free ions in the solution are completely removed ;

[0043] (2) Preparation of gelatin solution: add gelatin to phosphate buffered solution (PBS), and keep stirring until the gelatin is completely dissolved;

[0044] (3) Preparation of dialdehyde-based nanocellulose (DAC) / gelatin (GEL) composite hydrogel: blend a 5% DAC solution with a 5% GEL solution at a volume ratio of 6:4, rapidly After stirring evenly, incubate at 30°C for 4 hours;

[0045] (4) Printing of 3D tissue engineering scaffolds: DAC / GEL hydrogel is loaded into the barrel, the barrel temperature is 15°C, the platform temperature is 5°C, the needle diameter is 220μm, the extrusion pressure is 0.25Mpa, and the nozzle travel speed is 30mm / S, complete the 3D printing of the tissue engineering scaffold.

[0046] 4. Embodim...

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Abstract

The invention provides a 3D printing preparation method of a skin tissue engineering scaffold and an in vitro cell toxicity testing method of the scaffold. The 3D printing preparation method comprisesthe following steps: preparation of double aldehyde nano-crystalline cellulose used for a scaffold material, preparation of a gelatin solution, preparation of a double aldehyde nano-crystalline cellulose and gelatin compound hydrogel, and printing of the 3D tissue engineering scaffold. The problem that the tissue engineering scaffold needs the requirements of high porosity and high precision is solved by using a 3D biological printing technology. DAC is taken as a cross-linking agent, cross-linking occurs with GEL through a Schiff base reaction to form a network structure, so that the 3D printing tissue engineering scaffold has excellent mechanical property and is unlikely to crack, and simultaneously the additional value of plant fiber is also improved. The DAC / GEL hydrogel has good biocompatibility, is free of toxic and / or side effects or immunologic rejection, has biological activity besides degradation characteristic, and is extremely beneficial for the growth and differentiationof cells and the implementation of cell functions.

Description

technical field [0001] The invention relates to the preparation of a high-strength material used for printing skin tissue engineering scaffolds, and a 3D bioprinting process adapted thereto. Background technique [0002] The skin is trauma and ulcers caused by chronic diseases are the main causes of skin defects and loss of function. The development of tissue engineering technology provides new ideas for its treatment and has become a research hotspot in recent years. The steps of tissue engineering scaffolds prepared by traditional methods are cumbersome, the accuracy of each process is not easy to grasp, the performance of the scaffolds produced varies greatly, and the orientation and pores of the scaffolds cannot be changed at will. The emergence of 3D printing technology provides the possibility to solve the above problems, and has the advantages of rapid prototyping, sample standard, mass production, etc., and can print out scaffolds of different sizes by changing vario...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/50A61L27/52A61L27/56A61L27/58A61L27/60B33Y10/00B33Y70/00B33Y80/00C12Q1/02
CPCA61L27/20A61L27/222A61L27/50A61L27/52A61L27/56A61L27/58A61L27/60B33Y10/00B33Y70/00B33Y80/00G01N33/5014C08L1/02
Inventor 周骥平姜亚妮许晓东张琦赵国琦朱兴龙
Owner YANGZHOU UNIV
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