Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Drug-resistance bacteria resisting compound nano preparation and preparation method thereof

A nano-preparation and drug-resistant bacteria technology, which is applied in the direction of antibacterial drugs, pharmaceutical formulas, and medical preparations with non-active ingredients, can solve the problems of antibiotic aggravation, achieve low cost, high activity, and less drug resistance.

Active Publication Date: 2018-05-08
SOUTHEAST UNIV
View PDF2 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inappropriate use of antibiotics exacerbates this process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The composite nano-preparation of anti-drug-resistant bacteria of the present invention is prepared by the following method:

[0024] Step 1, dissolve egg yolk lecithin, cholesterol, and dihexadecyl phosphate in 10 mL of chloroform at a ratio of 5:3:1, then add a certain amount of erythromycin, fully dissolve, and rotate to evaporate to obtain a lipid film , hydrated and incubated for 1 hour, vortexed once every 10 minutes, and the hydrated suspension was sonicated in a water bath for 15 minutes, ultrafiltered, centrifuged, and concentrated to obtain liposomes;

[0025] Step 2, add appropriate amount of water and antimicrobial peptide KIWVIRWR to the liposome prepared in step 1, and assemble at 4° C. for 48 hours.

[0026] The initial concentration of antimicrobial peptide was 32 μg / mL, and the initial concentration of erythromycin was 32 μg / mL. Methicillin-resistant Staphylococcus aureus (MRSA) was cultured in MH broth containing oxacillin and sodium chloride respectiv...

Embodiment 2

[0028] The composite nano-preparation of anti-drug-resistant bacteria of the present invention is prepared by the following method:

[0029] Step 1: Dissolve dilinoleoylphosphatidylcholine, cholesterol, 1,2-dioleoyl-sn-glycerol-3-phosphoryl-rac-(1-glycerol) in 10mL at a ratio of 4:1:1 In the mixed solvent of chloroform / methanol, add a certain amount of doxycycline, fully dissolve and then rotary evaporate to obtain a lipid film, hydrate and incubate for 1 hour, vortex once every 10 minutes, and hydrate the suspension in a water bath Ultrasound for 15 minutes, ultrafiltration, centrifugation, and concentration to obtain liposomes;

[0030] Step 2, add appropriate amount of water and antimicrobial peptide RIWVIWRR to the liposome prepared in step 1, and assemble at 4°C for 24 hours.

[0031] Meropenem, doxycycline, and ciprofloxacin were selected to conduct in vitro induction experiments on Acinetobacter baumannii. Three treatment concentrations (selected from 1 / 4, 1 / 2, 1, 2 o...

Embodiment 3

[0034] The composite nano-preparation of anti-drug-resistant bacteria of the present invention is prepared by the following method:

[0035]Step 1, dissolve polyethylene glycol-modified dipalmitoylphosphatidylcholine, cholesterol, and phosphatidylglycerol in a 7:2:1 ratio in 10 mL of chloroform / methanol mixed solvent, and then add a certain amount of thiamphenicol, After fully dissolved, the lipid film was prepared by rotary evaporation, hydrated and incubated for 1 hour, vortexed once every 10 minutes, the hydrated suspension was ultrasonicated in a water bath for 15 minutes, ultrafiltered, centrifuged, and concentrated to obtain liposomes;

[0036] Step 2, add appropriate amount of water and antimicrobial peptide RIWVIRWR to the liposome prepared in step 1, and assemble at 4° C. for 24 hours.

[0037] The initial antimicrobial peptide concentration was 16 μg / mL, and the initial thiamphenicol concentration was 8 μg / mL. Methicillin-resistant Staphylococcus epidermidis (MRSE) w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a drug-resistance bacteria resisting compound nano preparation. The compound nano preparation is prepared by compounding a cationic anti-microbial peptide and an anionic lipidosome through an electrostatic interaction, wherein the sequence of the cationic anti-microbial peptide is one of RIVVIRVA, KIWVIRWR, RIWVIRWR, RIWVIWRR or RRWVIWRR; and an antibiotic drug is wrapped in the anionic lipidosome. According to the compound nano preparation, the anti-microbial peptide and the lipidosome drug jointly act on drug-resistance bacteria based on interference of the cationic anti-microbial peptide to a cell efflux mechanism, so that the original antibiotics resisting bacteria can exert the bactericidal effect, and the anti-microbial peptide and the antibiotics realize synergistic interaction to achieve the bactericidal effect; and therefore, the lowest inhibitory concentration of the anti-microbial peptide and the antibiotics in the compound nano preparation is greatlyreduced, and the anti-microbial activity of the compound nano preparation is effectively improved.

Description

technical field [0001] The invention relates to a composite nano-preparation for resisting drug-resistant bacteria, and also relates to a preparation method of the composite nano-preparation for resisting drug-resistant bacteria, belonging to the technical field of biomedical materials. Background technique [0002] With the continuous advent of new antibiotics and their widespread use around the world, bacterial resistance to antibiotics has become increasingly prominent, which has become a very serious global public health threat, especially superbugs (such as: New Delhi metal β-end The emergence of amidase pan-drug-resistant bacteria) is almost all resistant to existing antibiotics, which has aggravated people's concerns about drug resistance. [0003] Bacterial drug resistance is a way of survival for bacteria to adapt to changes in the environment. When antibiotics exist in the environment in which bacteria live, it will exert a selective pressure on bacteria, and only ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/08A61K9/127A61K47/24A61K45/06A61P31/04A61K31/7048A61K31/65A61K31/165
CPCA61K9/127A61K31/165A61K31/65A61K31/7048A61K38/08A61K45/06A61K47/24A61K2300/00
Inventor 姚琛周宾
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com