Liquid chromatographic analysis method for detecting content of diazepam in blood

Abstract

The invention discloses a method for detecting a drug concentration of diazepam in blood. According to the method, a standard solution is calibrated by using a liquid chromatographic analytic instrument and an ultraviolet detector; a standard curvilinear equation which is y=a*x+b is fitted and obtained; a to-be-detected blood sample is taken; after to-be-detected blood is treated, a to-be-detectedsample is detected by similarly using the liquid chromatographic analytic instrument and the ultraviolet detector, so as to obtain a value y of the to-be-detected blood; the y of the to-be-detected blood is substituted into the standard curvilinear equation; a relative concentration x of a target substance in the to-be-detected blood sample is obtained through calculation; a concentration of working fluid of an internal standard substance is known; thus, the drug concentration of the diazepam in the to-be-detected blood in the sample is calculated and obtained; according to the method, an internal standard method is combined with high performance liquid chromatography; the accuracy of a quantitative result is improved; a system error is eliminated; an analysis time is effectively shortened; a detection process is enabled to be simple, convenient and quick, and the monitoring carried out on a blood concentration of the diazepam in the body of a patient in clinical treatment is more facilitated.

Description

technical field [0001] The invention relates to the technical field of clinical blood drug concentration monitoring of psychotropic drugs, in particular to a method for detecting the concentration of diazepam in blood. Background technique [0002] Diazepam (Diazepam), also known as stability, is a commonly used benzodiazepine drug, which is often used as a pre-anesthesia drug, adjuvant drug and a component of compound general anesthesia in clinical practice. In addition, it is often used to control muscle spasms and convulsions, especially in combination with other antiepileptic drugs to treat seizures. The molecular formula of diazepam is C 16 h 13 ClN 2 O, the specific structural formula is as figure 1 shown. As a commonly used sedative in clinical practice, diazepam undergoes oxidative metabolism mainly through the action of hepatic cytochrome P450 enzymes in the body, and has genetic polymorphisms. Due to the large individual differences, narrow therapeutic window...

AI Technical Summary

Problems solved by technology

Among them, the imported reagents used in the immunological method are expensive, and the specificity of the kit used is slightly poor; the TLC-UV method is time-consuming and labor-intensive, and it is difficult to analyze a large number of samples; It has high requirements and is not suitable for widespread use in small and medium-sized hospitals; high performance liquid chromatography has the advantages of accuracy, sensitivity, strong specificity, good reproducibility, and low cost, and has become the main method for determining the blood concentration of diazepam

However, the pretreatment of the HPLC method currently used is more complicated, the analysis time is longer, and the consumption of organic solvents is more.

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