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Method for synchronously and rapidly detecting nine functional active components in Yedao deer and turtle wine

A technology of Yedao deer tortoise wine and active ingredients, which is applied in measurement devices, material separation, analysis of materials, etc., can solve problems such as time-consuming and labor-intensive, and achieve the effects of no sample loss, simple pretreatment, and reasonable design.

Active Publication Date: 2018-04-20
海南椰岛酒业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The active ingredients in Yedao Lugui Liquor are derived from the multi-flavored Chinese medicinal materials in the formula. According to literature review, most of the symbolic ingredients of traditional Chinese medicine are detected by high performance liquid chromatography, and diode array detectors and evaporative light scattering detectors are commonly used. To detect differences in wavelength and polarity, it is usually necessary to establish an independent separation method, which is often time-consuming and labor-intensive
On the other hand, the nutritional value of Yedao Lugui Wine has been mainly publicized by word of mouth, and its functional active ingredients have not been deeply explored through scientific means.

Method used

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  • Method for synchronously and rapidly detecting nine functional active components in Yedao deer and turtle wine
  • Method for synchronously and rapidly detecting nine functional active components in Yedao deer and turtle wine
  • Method for synchronously and rapidly detecting nine functional active components in Yedao deer and turtle wine

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Take Yedao Lugui Wine to pass through a 0.45 μm filter membrane, and then use the following liquid chromatography-mass spectrometry method for detection to obtain the extracted ion chromatogram of Yedao Lugui Wine in MRM mode.

[0047] Chromatographic conditions: the chromatographic column is Procell 120Columns SB-AQ 2.7μm; the mobile phase A is a mixed solution of formic acid aqueous solution and ammonium formate, the concentration of ammonium formate in the mixed solution is 5mmoL / L and the volume concentration of formic acid in formic acid aqueous solution is 0.1 vt%, the mobile phase B is methanol; the elution time and the volume content of methanol within the elution time are as follows: 0-1min, 0-2%; 1-2min, 2-40%; 2-5min, 40-70%; 5-9min, 70-95%; 9-11.5min, 95-95%; 11.5-12min, 95-2%; the injection volume is 2μL; the flow rate of the mobile phase is 0.4mL / min; the column temperature is 40°C.

[0048] Mass spectrometry conditions: the working mode of the electrospra...

Embodiment 2

[0052] Take Yedao Lugui Wine to pass through a 0.45 μm filter membrane, and then use the following liquid chromatography-mass spectrometry method for detection to obtain the extracted ion chromatogram of Yedao Lugui Wine in MRM mode.

[0053] Chromatographic conditions: the chromatographic column is Procell 120Columns SB-AQ 2.7μm; the mobile phase A is a mixed solution of formic acid aqueous solution and ammonium formate, the concentration of ammonium formate in the mixed solution is 4mmoL / L and the volume concentration of formic acid in formic acid aqueous solution is 0.05 vt%, the mobile phase B is methanol; the elution time and the volume content of methanol within the elution time are as follows: 0-1min, 0-2%; 1-2min, 2-40%; 2-5min, 40-70%; 5-9min, 70-95%; 9-11.5min, 95-95%; 11.5-12min, 95-2%; the injection volume is 1.5μL; the flow rate of the mobile phase is 0.3mL / min; the column temperature is 38℃ .

[0054] Mass spectrometry conditions: the working mode of the electro...

Embodiment 3

[0058] Take Yedao Lugui Wine to pass through a 0.45 μm filter membrane, and then use the following liquid chromatography-mass spectrometry method for detection to obtain the extracted ion chromatogram of Yedao Lugui Wine in MRM mode.

[0059] Chromatographic conditions: the chromatographic column is Procell 120Columns SB-AQ 2.7μm; the mobile phase A is a mixed solution of formic acid aqueous solution and ammonium formate, the concentration of ammonium formate in the mixed solution is 6mmoL / L and the volume concentration of formic acid in formic acid aqueous solution is 0.15 vt%, the mobile phase B is methanol; the elution time and the volume content of methanol within the elution time are as follows: 0-1min, 0-2%; 1-2min, 2-40%; 2-5min, 40-70%; 5-9min, 70-95%; 9-11.5min, 95-95%; 11.5-12min, 95-2%; the injection volume is 2.5μL; the flow rate of the mobile phase is 0.5mL / min; the column temperature is 42℃ .

[0060] Mass spectrometry conditions: the working mode of the electro...

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Abstract

The invention relates to a method for synchronously and rapidly detecting nine functional active components in Yedao deer and turtle wine and belongs to the field of researches of functional components of health-care wine. The method comprises the following steps: carrying out qualitative analysis on the active components in the Yedao deer and turtle wine by adopting a liquid chromatography-mass spectrometry method through a positive ion scanning working mode; then carrying out quantitative analysis by adopting an external standard method. The method provided by the invention has the advantages of simple pre-treatment steps, high precision, low quantification limit and good stability and can be used for rapidly determining the content of Chinese yam sapogenin, 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside, betaine, ligustilide, scopoletin, atractylenolide III, emodin, emodin methyl ether and codonopsis pilosula alkyne glucoside in a Yedao deer and turtle wine sample; the method can be used for synchronously and rapidly determining the components in the Yedao deer and turtle wine, a Chinese herbal medicine mixed extracting solution of a similar formula or prepared wine.

Description

technical field [0001] The invention relates to the field of research on functional components of health-care wine, and in particular to a method for simultaneously and quickly detecting nine functional active components in Yedao Lugui wine. Background technique [0002] Yedao Lugui Wine is derived from the addition and subtraction of Guilu Erxianjiao and Bazhen Decoction in ancient recipes. It is a precious intangible cultural heritage. It combines the advantages of the two and optimizes the formula on this basis. Scientific animal experiments have proved that it has health effects of anti-fatigue and immune regulation. There are as many as a dozen kinds of medicinal materials in the prescription of Luguijiu (animals and plants), with more than 120 kinds of active compounds, including diosgenin, 2,3,5,4-tetrahydroxystilbene glucoside, betaine, ligusticum Lactone, scopoletin, Atractylodes lactone III, emodin, emodin methyl ether, and tangshenoside have important physiologic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 江伟展学孔雷燕王淑军张湘娥吴炜张翠侠
Owner 海南椰岛酒业发展有限公司
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