Actinomucor elegans strain used for producing lipase from vegetable oil and fat deodorizing distillates

Abstract

The invention belongs to the technical field of biological fermentation engineering, and more specifically relates to actinomucor elegans strain used for producing lipase from vegetable oil and fat deodorizing distillates. The actinomucor elegans strain belongs to gc-1 actinomucor elegans, and the preservation number is GDMCC No.60214. The bacteria strain is capable of taking vegetable oil and fatdeodorizing distillates as a single carbon source, can be used for fermentation production of lipase taking the vegetable oil and fat deodorizing distillates as raw materials; the highest enzyme activity is 10.89U / ml, and the application prospect is promising. The related raw materials are cheap; the preparation technology is simple and convenient; no environment pollution is caused; the economical benefits are excellent; and the economical value and the popularization application meaning are excellent.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering, and specifically relates to a patent application for a mucormyces radiata strain that uses vegetable oil deodorized distillates to produce lipase. Background technique [0002] Deodorization distillate is a by-product collected during steam distillation of vegetable oil deodorization process or physical refining process. This substance has the appearance and consistency similar to acid residue, so it is often attributed to scum oil, mainly tocopherol, A complex fatty acid mixture of sterols, sterol esters, fatty acid glycerides, carbohydrates and other impurities. The content of each component varies greatly depending on the type of vegetable oil and the production process. At present, many domestic vegetable oil deodorization distillates are treated as waste by manufacturers, which not only causes a huge waste of raw materials, but also causes environmental pollution....

AI Technical Summary

Problems solved by technology

But also there are some weak points in prior art, main reason is: one, sterol, vitamin E and free fatty acid, glyceride combine together, and all are fat-soluble compound, the physicochemical property difference between them is little, Therefore, it is very difficult to separate sterols and vitamin E from them, which undoubtedly increases the difficulty and cost of extraction; secondly, as a by-product, the content of sterols and vitamin E in the deodorized distillate is actually very little, and the content of different vegetable oils The composition of deodorized distillates is different, and the content of vitamin E and sterols is also different. For example, in the deodorized distillates of rice bran oil and cottonseed oil, the average content of sterols is only about 1-4%, and some vegetable oil deodorized distillates It even contains almost no sterols and vitamin E, which makes some deodorized distillates have no extraction value at all, so they are directly disposed of as waste; third, the extraction and purification of sterols and vitamin E adopt vacuum distillation, molecular distillation, solvent extraction, Methods such as supercritical extraction, these methods or processes are complicated, require large investment, or require expensive equipment, high production costs, or consume toxic and flammable organic reagents, which are not only harmful to health, but also cause environmental pollution

Due to the above technical reasons, the actual utilization rate of the deodorized distillate is low

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