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A kind of Paenibacillus polymyxa biocontrol strain af01 and its application

A technology of Paenibacillus polymyxa and bacillus, applied in Paenibacillus polymyxa AF01 and its application field, can solve safety problems, pollute the environment, pesticide residues and other problems, achieve the effect of reducing the occurrence of diseases and good development prospects

Active Publication Date: 2021-04-06
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, postharvest diseases of dragon fruit are mainly controlled by chemical agents, but long-term use of chemical pesticides will cause pathogenic bacteria to develop drug resistance, and bring about environmental pollution and safety problems of pesticide residues. Therefore, the development and utilization of biocontrol bacteria that exist in nature has great potential important practical significance

Method used

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  • A kind of Paenibacillus polymyxa biocontrol strain af01 and its application
  • A kind of Paenibacillus polymyxa biocontrol strain af01 and its application
  • A kind of Paenibacillus polymyxa biocontrol strain af01 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 : Screening of strains

[0035] (1) Primary screening of plate medium: Accurately weigh 5g of pitaya orchard soil samples, add 50ml of sterile water, place in a shaker at room temperature and shake for 30-60min at a shaking rate of 200rpm. After standing for 30 min, the supernatant was taken for serial dilution. Spread the gradient dilutions on the plate screening medium respectively, incubate upside down at 28-37°C for 2-4 days, observe regularly, purify the bacterial colonies with obvious differences in appearance, shape and color, and save them for future use.

[0036] (2) Using the plate confrontation growth method, inoculate a strain of Pitaya canker bacterium Arthrosporum neocylindrum on the PDA plate as an indicator bacterium, and carry out antagonistic screening on the bacterial strains isolated and purified from step (1). The antagonistic strains were kept for future use. Screen the strains with strong antibacterial ability against Arthrosporum ne...

Embodiment 2

[0038] Example 2 : Identification of strains

[0039] (1) Morphological analysis of strains

[0040] Such as figure 1 As shown, AF01 Gram-positive bacteria are rod-shaped, spores are oval, and the cell diameter is greater than 1 μm.

[0041] Such as figure 2 As shown, the colony of strain AF01 on the PDA medium is viscous, raised and convex, with smooth edges, round, milky white or transparent.

[0042] The bacterial strain can utilize glucose, xylose, L-arabinose, mannitol and lactose, can utilize citrate, reduce nitrate, can hydrolyze starch and decompose casein, and react with enzymes. It does not grow under the conditions of 50°C and pH5.7, nor does it grow under 7% NaCl solution, and methyl red reaction occurs.

[0043] Combined with the test results of morphological characteristics, cultural characteristics and physiological and biochemical characteristics, the strain AF01 was preliminarily determined to be Paenibacillus ( Paenibacillus sp. ).

[0044] (2) Mol...

Embodiment 3

[0048] Example 3 : Inhibitory effect of Paenibacillus polymyxa AF01 on hyphal growth of postharvest dragon fruit disease pathogen

[0049] The post-harvest disease pathogens of dragon fruit cultured for 3-6 days were removed from the center of the PDA plate with a 5 mm hole punch, and placed in the center of the PDA plate at a distance from the fungus. Point 1 μL with a pipette gun at 2.5cm of the cake, the concentration is 10 9 Cfu / mL AF01 culture solution was cultured in a 28°C incubator, and the inhibition zone or zone was observed.

[0050] As shown in Figure 2-6, the culture medium of Paenibacillus polymyxa AF01 can inhibit the mycelia growth of Arthrophyllum neocylindrosum, Fusarium, Botrytis cinerea, Alternaria, and Helminthosporium cactus, and the inhibition rate is shown in Table 1.

[0051] Table 1 The inhibitory rate of AF01 suspension to pathogenic bacteria

[0052]

[0053] It can be seen from Table 1 that after 3-7 days of cultivation, the AF01 bacterial a...

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Abstract

The invention belongs to the technical field of microorganism application, and in particular relates to Paenibacillus polymyxa AF01 and its application. A Paenibacillus polymyxa AF01, its taxonomic name is Paenibacillus polymyxa AF01, the preservation number GDMCC No.60281, the preservation date is November 20, 2017, and the preservation unit is Guangdong Microbial Culture Collection Center. The Paenibacillus polymyxa AF01 inoculum provided by the invention can effectively reduce the occurrence of postharvest diseases of pitaya, has no phytotoxicity to pitaya, has no pollution to the environment, and has good prospects for the development of biological pesticides.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to Paenibacillus polymyxa AF01 and its application. Background technique [0002] Dragon fruit Hylocereus undatus, also known as red dragon fruit (Taiwan), xianmiguo, sesame fruit, etc., belongs to the plant of the genus Cactaceae. It is native to tropical Central America. It was introduced to Taiwan for trial planting in the early 1990s. After domestication and improvement, it has been successively cultivated and promoted in Guangdong, Hainan, Fujian, Guangxi and other provinces and regions in my country. At present, Guangxi has become the largest dragon fruit producing area in the country. Dragon fruit has a unique fruit shape and good industrialization prospects. In addition to eating fresh, it can also be used for wine making, canning, jam, etc. The fruit is rich in nutrients such as protein, amino acids, mineral elements, vitamins and fatty acids, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/01
CPCA23B7/155C12N1/20C12N1/205C12R2001/01
Inventor 林珊宇朱桂宁贤小勇韦小妹
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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