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Dienzymatic extraction method of corn cervi pantotrichum

An extraction method, double enzymatic hydrolysis technology, applied in the direction of food science, etc., can solve the problems of strong fishy smell, low extraction rate, difficulty in taking velvet antler, etc., and achieve the effect of increased extraction rate, high solubility, and retained nutrients

Inactive Publication Date: 2018-03-06
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the application of velvet antler is still in the initial processing stage of raw materials, such as: taking slices directly, decocting or soaking in wine
It is difficult to take velvet antler, and it has a strong fishy smell. Therefore, it is difficult to meet the needs of modern consumers by taking it directly.
Although there are velvet extract, velvet oral liquid and other extraction products on the market, most of them are extracted by traditional boiling method. Since velvet belongs to animal tissue, only a very small amount of ingredients can be used in water or alcohol, so the traditional boiling method is used. The extraction rate of the method is very low

Method used

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  • Dienzymatic extraction method of corn cervi pantotrichum
  • Dienzymatic extraction method of corn cervi pantotrichum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] This embodiment provides a double enzymatic extraction method for dried velvet antler, which is realized by the following steps:

[0036] 1) Grinding by colloid mill: dehairing and slicing the dried velvet antler; then add distilled water according to the mass ratio of dry velvet antler: water = 1:9, and grind through a colloid mill. The particle size of the colloid mill is 10μm-50μm.

[0037] 2) Digestion: Adjust the pH of the ground liquid to 2.0 with 10M concentrated HCl, heat it to 37°C in a water bath, and maintain a constant temperature; add pepsin at a ratio of dry velvet antler: pepsin = 50:1 (mass ratio), Stir uniformly, digest at 37°C for 12 hours; increase the temperature to above 95°C for at least 15 minutes to inactivate the enzyme; then lower the temperature to 37°C, adjust the pH to 8.4 with 20M concentrated NaOH, press dry velvet: trypsin Add trypsin at a ratio of 175:1 (mass ratio), stir evenly, and digest at 37°C for 2 hours; increase the temperature to abo...

Embodiment 2

[0041] This embodiment provides a double enzymatic extraction method for dried velvet antler, which is achieved by the following steps:

[0042] 1) Grinding by colloid mill: dehairing and slicing the dried velvet antler; then add distilled water according to the mass ratio of dry velvet antler: water = 1:9, and grind through a colloid mill. The particle size of the colloid mill is 10μm-50μm.

[0043] 2) Digestion: Adjust the pH of the ground liquid to 2.0 with 10M concentrated HCl, heat it to 37°C in a water bath, and maintain a constant temperature; add pepsin at a ratio of dry velvet antler: pepsin = 55:1 (mass ratio), Stir uniformly, digest at 37°C for 12 hours; increase the temperature to above 95°C for at least 15 minutes to inactivate the enzyme; then lower the temperature to 37°C, adjust the pH to 8.4 with 20M concentrated NaOH, press dry velvet: trypsin Add trypsin at a ratio of 180:1 (mass ratio), stir well, and digest at 37°C for 2 hours; increase the temperature to above...

Embodiment 3

[0047] This embodiment provides a double enzymatic extraction method for dried velvet antler, which is achieved by the following steps:

[0048] 1) Grinding by colloid mill: dehairing and slicing the dried velvet antler; then add distilled water according to the mass ratio of dry velvet antler: water = 1:9, and grind through a colloid mill. The particle size of the colloid mill is 10μm-50μm.

[0049] 2) Digestion: Adjust the pH of the ground liquid to 2.0 with 10M concentrated HCl, heat it to 37°C in a water bath, and maintain a constant temperature; add pepsin at a ratio of dry velvet antler: pepsin = 65:1 (mass ratio), Stir uniformly, digest at 37°C for 12 hours; increase the temperature to above 95°C for at least 15 minutes to inactivate the enzyme; then lower the temperature to 37°C, adjust the pH to 8.4 with 20M concentrated NaOH, press dry velvet: trypsin Add trypsin at a ratio of 190:1 (mass ratio), stir well, and digest at 37°C for 2 hours; increase the temperature to above...

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Abstract

The invention relates to a dienzymatic extraction method of corn cervi pantotrichum and belongs to the technical field of deep processing of special economic animal deer products. The method providedby the invention comprises the following steps: removing hair from fresh corn cervi pantotrichum or dry corn cervi pantotrichum, cutting into slices and adding distilled water to perform grinding; digesting the ground liquid by using pepsase and trypsin sequentially, wherein the mass ratio of the dry corn cervi pantotrichum substance to the pepsase is (50-70):1, and the mass ratio of the dry corncervi pantotrichum substance to the trypsin is (175-200):1; and performing drying treatment on the digested liquid to obtain a corn cervi pantotrichum extract. The extraction rate of the corn cervi pantotrichum exceeds 75 percent and is increased by nearly 4 times compared with that of the traditional extraction method. The corn cervi pantotrichum extract extracted by the method can be used for preparing health-care products.

Description

Technical field [0001] The invention relates to a double enzymatic extraction method for antler antler, belonging to the technical field of deep processing of special economic animal deer products. Background technique [0002] The unossified young horns of male sika deer or red deer with antler, is a valuable traditional Chinese medicine in my country, and it is as famous as ginseng and is known as the first animal medicine. "Compendium of Materia Medica" says: Health essence and marrow, nourish blood and nourish yang, strengthen muscles and bones, cure all wastage and dysentery. It is often used to "strengthen the vital energy, replenish qi and blood, nourish the essence, strengthen the bones and muscles. To treat asthenia, fatigue, dizziness, deafness, dark eyes, waist and knee pain, impotence, hydration, cold uterus, uterine bleeding, Take it." Now biology believes: velvet antler can enhance nerve excitability, promote the restoration of the nervous system and improve the fu...

Claims

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Application Information

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IPC IPC(8): A23L33/00A23L13/20
CPCA23L13/20A23L33/00
Inventor 赵海平李春义齐晓妍刘振
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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