A cell culture dish used for staining observation, and a cell culture staining and observing method

Abstract

A cell culture dish used for staining observation, and a cell culture staining and observing method are disclosed. The cell culture dish includes a culture dish body and a culture dish cover. One sideof the cover dish body is provided with a handle. The culture dish body includes a bottom plate. The bottom plate is provided with a plurality of bottom grooves. The upper periphery of the bottom plate is provided with an enclosed vertical sealing plate. The handle is connected to the bottom plate. The lower end surface of the culture dish cover is matched with the upper end surface of the vertical sealing plate. The method includes 1) preparing a culture liquid, 2) culturing cells, 3) staining, and 4) performing microscopic examination. A step of adding a glass sheet into a culture hole is not needed, and a cell suspension is directly added into the cell culture dish specially manufactured, and therefore glass sheet floating and glass sheet clamping problems are avoided. After staining is finished, the bottom wall of the culture dish is put below and observed by an inverted microscope directly, or the bottom wall of the culture dish is turned over and observed by an upright microscope, and therefore cell layers and a microscope lens are very close. The cell culture dish and the method are particularly suitable for observation by high-power oil immersion objectives.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a cell culture dish for staining and observation and a cell culture staining and observation method. Background technique [0002] As an important research method, cell culture is widely used in nutrition, pathology, environmental toxicology, cell biology, oncology, genomics, genetics and resource protection, and has become an indispensable technical means in scientific research . In experiments, staining (such as immunochemistry, immunofluorescence, hematoxylin-eosin, etc.) is often required to observe cell morphology and structure, so it is necessary to culture cells on a suitable carrier to facilitate observation under a microscope . [0003] At present, the method of cell "climbing" is usually used for staining and observation, that is, the coverslip is first rinsed, sterilized and placed in the well of the culture plate or culture dish, and the cell suspension is adde...

AI Technical Summary

Problems solved by technology

However, in the experiment, it was found that no matter whether the cover glass or the special glass slide was used, the following three problems existed: (1) the slide was placed in the culture well, and after adding the cell suspension, it was found that the slide would float, causing the cells to Adhesive growth on the upper and lower sides of the glass slide, which is not conducive to observing cell morphology under a microscope

Even if a small amount of culture solution is first dropped on the place where the slides are to be placed in the culture wells, and then the slides are placed gently, so that the slides and the culture wells seem to stick together, but some slides float after adding the cell suspension

(2) There are slight differences in shape and function between the cells grown on the glass slide and the cells cultured in the culture well. The cells grown on the glass slide deviate from the normal cell shape and their secretory function is reduced. For example, the formation of lipid droplets in adipocytes slows down.

(3) Take out the glass slide with tweezers after the staining process is completed. Since the glass slide is thin, it is difficult to control the strength when clamping.

Images