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Disease-State Biomarker For Renal Disease

A morbid and kidney injury technology, applied in biological testing, biological material analysis, disease diagnosis, etc., can solve the problem of no display of diagnostic markers, and achieve the effect of early detection

Inactive Publication Date: 2018-02-09
KANAZAWA UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Further disclosed is selected from D-serine, D-threonine, D-alanine, D-asparagine, D-allothreonine, D-glutamine, D-proline and D-phenylalanine One or two or more amino acids in amino acids can be used as a pathological indicator value for kidney disease (Patent Document 1). In this document, biological materials such as blood, plasma, and urine are listed as biological materials for samples, and body fluids such as feces, sweat, etc. , nasal mucus and other excreta, body tissues such as body hair, nails, skin tissue, and internal organ tissue, but there is no indication of the actual use of D-isomer amino acids in feces as diagnostic markers

Method used

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  • Disease-State Biomarker For Renal Disease
  • Disease-State Biomarker For Renal Disease
  • Disease-State Biomarker For Renal Disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0098] Materials and methods

[0099] research ethics

[0100] All experiments were performed in accordance with facility guidelines and were performed with the approval of the facility's animal experimentation committee.

[0101] Material

[0102] Amino acid enantiomers and HPLC grade acetonitrile were purchased from Nacalai Tesque (Kyoto). HPLC-grade methanol, trifluoroacetic acid, boric acid, etc. were purchased from Wako Pure Chemical Industries (Osaka). Water was purified using a Mill-Q gradient A10 system.

[0103] Animal Model of Mild Acute Kidney Injury Made by Cutting Renal Artery and Vein

[0104] The skin and peritoneum of 6-week-old C57BL / 6JJic male mice (Clear Japan) were incised under anesthesia. Renal artery and vein shearing was performed for 40 minutes with incubation on a hot plate at 37°C. Cutting is complete and the peritoneum and skin are sutured. Ischemic kidney injury is induced by renal artery and vein shearing. A kidney injury induction...

Embodiment 2

[0131] Materials and methods

[0132] research ethics

[0133] The present inventors obtained stool samples and blood samples from 8 patients with chronic renal failure and 3 healthy persons at the Kanazawa University Hospital. Informed consent was obtained from these subjects, and the experiment was conducted in accordance with a test approved by the Medical Ethics Review Committee of Kanazawa University.

[0134] Material

[0135] Amino acid enantiomers and HPLC grade acetonitrile were purchased from Nakalai Tesque (Kyoto). HPLC grade methanol, trifluoroacetic acid, boric acid, etc. were purchased from Wako Pure Chemical Industries (Osaka). Water was purified using a Mill-Q gradient A10 system.

[0136] The feces collected from 8 patients with chronic renal failure and 3 healthy persons were dissolved in methanol and supplied to the amino acid stereoisomer analysis of the D, L-amino acid simultaneous high-sensitivity analysis system (Japanese Patent No. 4291628) de...

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Abstract

The present invention addresses the problem of developing an analytical method which makes it possible to diagnose early or mild renal disorders. The method is based on calculating a disease-state index value for renal disorders on the basis of the quantities of D-form and / or L-form amino acids, from feces or intestinal content. By comparing the disease-state index value with a threshold value determined from the disease-state index values of a renal failure patient group and a healthy subject group, it is possible to diagnose a mild renal disorder patient group.

Description

technical field [0001] The present invention relates to an analysis method including a step of calculating a kidney damage morbidity index value from feces or intestinal contents based on the amino acid amounts of D-isomer and L-isomer, a method for examining kidney damage, and an output A sample analysis system for pathological information about kidney damage. Background technique [0002] The kidney is an organ whose main function is to maintain the stability of body fluids by filtering waste from the blood and excreting excess water as urine. The kidneys are damaged due to immune system abnormalities, allergic reactions to drugs, high blood pressure, diabetes, hemorrhage and / or a sudden drop in blood pressure, infectious diseases, dehydration accompanying burns, etc., resulting in decreased kidney function. Renal failure is called renal failure when the renal function drops below about 60% due to renal damage, and is broadly classified into acute renal failure (AKI) and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6806G01N2800/347G01N33/6893
Inventor 和田隆志古市贤吾坂井宣彦岩田恭宜原章规中出祐介浜濑健司三次百合香中根舞子三田真史
Owner KANAZAWA UNIV
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