PCR-specific primers, kits and methods of use for detection of thyroid cancer metastasis-related genes

Abstract

The invention discloses a PCR-specific primer, a kit and a use method thereof for detecting genes related to metastasis of thyroid cancer. The high-throughput sequencing technology-based PCR-specific primers for the detection of thyroid cancer metastasis-related genes independently developed by the present invention, the kit and the use method involved in the thyroid cancer metastasis-related genes have been verified by samples and can be used to detect thyroid cancer The mutation status of metastasis-related genes, the test result can be used as an intermediate result together with other test results to determine whether cancer cells have metastasized or whether there is a possibility of metastasis or recurrence, which is of great significance for the treatment and prognosis of thyroid cancer. Moreover, the present invention uses high-throughput gene sequencing technology to break through the limitations of traditional detection technology, improves detection sensitivity, and can detect even low-abundance mutated genes, thereby helping to predict the possibility of proliferation and recurrence of thyroid cancer cells. Significance.

Description

technical field [0001] The invention relates to the field of molecular biology, in particular to a PCR-specific primer for detecting thyroid cancer metastasis-related genes, a kit and a use method thereof. Background technique [0002] The thyroid is the largest endocrine organ in the human body, and thyroid cancer is a common endocrine tumor. Thyroid tumors are divided into benign and malignant, among them, malignant thyroid tumors include papillary carcinoma, follicular carcinoma, medullary carcinoma and undifferentiated carcinoma. Differentiated thyroid cancer accounts for 90% of all thyroid cancers. Undifferentiated thyroid cancer is rare, but it has a high degree of malignancy and poor prognosis. [0003] In the past 30 years, the incidence of thyroid cancer in most regions of the world, including my country, has been on the rise. In 2010, the incidence rate of thyroid cancer in my country was 4.12 / 100,000, male was 1.93 / 100,000, and female was 6.42 / 100,000; the morta...

AI Technical Summary

Problems solved by technology

Conventional serial section HE staining method has low sensitivity and needs to cut dozens or even hundreds of sections, which has been rarely used in recent years; immunohistochemical method is relatively simple to operate, with high sensitivity and specificity, but it is time-consuming and prone to False positive and false negative; the advantages of flow cytometry are fast speed, high precision, and good accuracy, but the disadvantage is that specific fluorescent dyes are required and the cost of instruments and reagents is high; PCR / RT-PCR technology is amplified Tumor cell marker genes or target RNA are used to detect tumor metastasis. The biggest advantage is high sensitivity, but the PCR method can only be designed for one or several known mutation sites, and cannot be used to detect unknown mutations; Qualcomm Quantitative sequencing is a next-generation sequencing technology that can perform high-throughput sequencing of multiple samples. It is time-consuming, high-precision, and large in data volume. It can detect both known mutation sites and unknown mutations.

[0006] Although high-throughput sequencing technology has been widely used in the detection of unknown mutations, there are still no effective, accurate and easy-to-use targeted mutations due to the limitations of numerous genes related to thyroid cancer metastasis and the design of amplification primers. PCR-specific primers for detection of genes related to metastasis of thyroid cancer and related detection methods

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