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Colloidal gold test strip for detecting human surfactant protein A and its preparation method and application

A surface-active protein, colloidal gold test paper technology, applied in the field of medical testing, can solve the problems of reducing the mortality rate of neonatal respiratory distress syndrome, and achieve the effects of sensitivity and specificity, convenient use and simple operation

Active Publication Date: 2019-03-12
徐立
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no colloidal gold test strip based on the detection of surfactant protein A for the rapid detection of neonatal respiratory distress syndrome, so a colloidal gold test strip for the rapid detection of neonatal respiratory distress syndrome is developed , has important clinical significance, can meet the urgent needs of clinical pediatrics and obstetrics, and is conducive to the preventive administration of neonatal respiratory distress syndrome, thereby reducing the mortality rate of neonatal respiratory distress syndrome

Method used

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  • Colloidal gold test strip for detecting human surfactant protein A and its preparation method and application
  • Colloidal gold test strip for detecting human surfactant protein A and its preparation method and application
  • Colloidal gold test strip for detecting human surfactant protein A and its preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0051] Embodiment 1 detects the colloidal gold test strip of human surfactant protein A

[0052] The colloidal gold test strip for detecting human surfactant protein A is composed of water-absorbing pad 7, nitrocellulose membrane 3, immune colloidal gold pad 2, sample pad 1 and polyvinyl chloride bottom plate 6 in sequence; the nitrocellulose membrane is wrapped with Quality control line C line 5 and detection line T line 4, C line is the secondary antibody, T line is rabbit anti-human surfactant protein A polyclonal antibody II; the immune colloidal gold pad is coated with colloidal gold labeled mouse antibody Glass fibers of human surfactant protein A monoclonal antibody I.

Embodiment 2

[0053] Embodiment 2: the preparation method of the colloidal gold test strip that detects human surfactant protein A

[0054] Preparation of colloidal gold:

[0055] 1. Add 48.5ml of deionized water into a 100ml round bottom flask, stir and boil, and use a clean condenser to reflux for 10min.

[0056] 2. Take 0.5ml of HAuCl respectively 4 The aqueous solution (1wt%) was added to 1ml of trisodium citrate aqueous solution (0.35-1.485wt%), stirred evenly to form a 2.5ml mixed solution, and then quickly added to the boiling water in step 1. It can be observed that the color of the solution changes rapidly from colorless to blue-gray, and finally turns into bright red through purple transition.

[0057] 3. The reaction solution was heated, stirred and refluxed for 1 hour, then the heating was stopped, and the temperature was naturally lowered to room temperature while stirring.

[0058] Preparation of colloidal gold labeling complex:

[0059] 1. Add 1ml of colloidal gold soluti...

Embodiment 3

[0079] Embodiment 3: the sensitivity analysis of the colloidal gold test paper strip prepared by embodiment 2

[0080] Get 25 colloidal gold test strips for the detection of human surfactant protein A made in the above-mentioned embodiment 2. Dissolve the human surfactant protein A antigen gradient in the standard diluent, the concentrations are 1000ng / ml, 500ng / ml, 400ng / ml, 200ng / ml, 0ng / ml, test 5 strips for each sample concentration, and the test time is 10 minute. Table 1 shows the visual interpretation results. The results show that when the content of human surfactant protein A in the detection sample solution is lower than 200ng / ml, the test strip of the present invention does not develop color, that is, the detection rate is 0. When the content of human surfactant protein A in the detection sample solution is more than or equal to 400ng / ml, the test strip can be detected and interpreted normally, and the detection rate is 100%. The results show that the colloidal g...

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Abstract

The invention belongs to the field of medical examination and relates to a colloidal gold test strip for detecting a human surfactant protein A, as well asa preparation method and application of the colloidal gold test strip.The colloidal gold test strip for detecting the human surfactant protein A comprises a water absorption pad, a nitrocellulose membrane, an immune colloidal gold pad, a sample pad and a polyvinyl chloride bottom platesequentially, wherein the nitrocellulose membrane comprises a quality control line C and a detection line T; the line C is a secondary antibody; the line T is rabbit anti-human surfactant protein A polyclonal antibody II; and the immune colloidal gold pad is a glass fiber coated with a colloidal gold labeled murine anti-human surfactant protein A monoclonal antibody I.The colloidal gold test strip can quickly detect the human surfactant protein A in body fluid, has sensitivity and specificity, is simple to operate, convenient to use and short in time, and can be applied to early detection or diagnosis products of a neonatal respiratory distress syndrome and products for detecting the human surfactant protein A in other body fluids.

Description

technical field [0001] The invention belongs to the field of medical testing, and relates to a colloidal gold test strip for detecting human surfactant protein A, a preparation method and application thereof. Background technique [0002] Neonatal respiratory distress syndrome (Neonatal Respiratory Distress Syndrome, NRDS) is a serious disease of early neonatal death caused by the lack of pulmonary surfactant. Its fatality rate is extremely high, and it is mainly seen in premature infants. The goal of prevention and treatment of neonatal respiratory distress syndrome is to intervene as early as possible to improve the survival rate as much as possible while minimizing potential adverse reactions. Since the advent of pulmonary surfactant (PS) replacement therapy, although the mortality rate of NRDS has dropped to 20%, neonatal respiratory distress syndrome is still the main cause of neonatal death. Although the application of pulmonary surfactant can reduce the incidence of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/558G01N33/531
CPCG01N33/531G01N33/558G01N33/577G01N33/6803G01N2800/12G01N2800/38
Inventor 陈晨语
Owner 徐立
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