Method for rapidly determining arabitol and mannitol in aerosol

Abstract

The invention relates to the technical field of atmospheric chemical analysis test, and especially discloses a method for rapidly determining arabitol and mannitol in aerosol. The method comprises thefollowing steps: 1, preprocessing a sample: ultrasonically extracting arabitol and mannitol from an aerosol sample by using ultrapure water, and filtering the obtained solution; 2, determining the sample: taking a liquid chromatogram with the chromatographic column being an anion exchange column, and carrying out mass spectrometry multiple reaction monitoring analysis by using a mass analyzer: selecting and simultaneously detecting a plurality of parent ion pairs and daughter ion pairs, allowing the parent ions with the mass-to-charge ratio being 151.0 and 181.0 to undergo first-level mass spectrometry and enter a bumping chamber, and allowing ions of chips broken by the bumping chamber, with the mass-to-charge ratio being 71.0, 59.0 and 89.1, to undergo second-level mass spectrometry, wherein the same parent and daughter ion pairs have same compound parameters; and 3, processing data: qualitatively detecting the arabitol and the mannitol, and respectively quantifying the arabitol andthe mannitol with 151.0-71.0 and 181.0-89.1 ion pairs with highest abundance. The method has the advantages of fast detection speed and low cost.

Description

technical field [0001] The invention relates to the technical field of atmospheric chemical analysis and testing, in particular to a method for rapidly measuring arabitol and mannitol in aerosols. Background technique [0002] The diffusion and spread of fungal spore aerosols in the atmosphere can cause acute and chronic human diseases, animal and plant diseases, and even affect the yield of crops. It can also indirectly affect global climate change, and has a potentially important impact on atmospheric chemical and physical processes. Therefore, it is very important to use molecular tracers to study the influence of fungal spore aerosol on regional or local atmospheric environment. [0003] In recent years, tracers of fungal spores have been used to determine and calculate the contribution of such fungi to atmospheric aerosols and to conduct source apportionment. Arabitol and mannitol are widely used fungal spore tracers, and their concentrations have a high correlation wi...

AI Technical Summary

Problems solved by technology

Gas chromatography uses an organic solvent to extract the target substance in the aerosol, and then detects it by gas chromatography-mass spectrometry after derivatization. This method needs to use an organic solvent for extraction, which consumes a lot of solvent and has high detection costs. In addition, sample preparation is time-consuming, and derivatization can also cause loss of analytes

In addition, the detection limit of arabitol and mannitol in the gas chromatography-mass spectrometry method reported in the literature is only 130-360 μg L -1 The detection limit cannot meet the detection requirements of low-concentration aerosol samples, and has limitations for detecting aerosol samples in areas less polluted by aerosols or in weather with low aerosol concentrations

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