Sodium alginate-chitosan polyionic compound hydrogel and its 3D-printing preparation method

Abstract

The invention discloses a sodium alginate-chitosan polyionic compound hydrogel and its 3D-printing preparation method. The compound hydrogel is the sodium alginate-chitosan polyionic compound hydrogelof which carboxylate radicals are crosslinked with ammonium ions, the biocompatibility is good, cell loss caused by material degradation in the cell culture process using hydrogel blocks can be effectively improved, and cells cultured on the materials easily obtain required oxygen and nutrient substances. The 3D-printing preparation method includes the following steps of preparation of a sodium alginate-chitosan polyionic compound solution and preparation of the sodium alginate-chitosan polyionic compound hydrogel. The sodium alginate-chitosan polyionic compound hydrogel is prepared by usingthe 3D-printing technology, the outside dimensions are controllable, pore structures which are visible in the macroscopic aspect and interconnected are obtained, combination between hydrogel materialsand tissue is easily achieved, and after material implantation, wear of materials at a tissue loss site is reduced.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a sodium alginate-chitosan polyion complex hydrogel and a 3D printing preparation method thereof. Background technique [0002] Sodium alginate is a polyanionic polysaccharide sodium salt extracted from brown algae. It has good biocompatibility and can electrostatically interact with divalent anions or polyanionic macromolecules to form a three-dimensional hydrogel network structure. Chitosan is a macromolecule produced by the deacetylation of methyl gram molecules. Due to the interaction between the amino groups on the molecular chain, the macromolecules of chitosan form crystals in water, making chitosan insoluble in water. In acid solution, the amino groups on the molecular chain of chitosan are transformed into ammonium ions to form polyanion macromolecules, which can electrostatically interact with polycations or cationic small molecules to form a three-dimensio...

AI Technical Summary

Problems solved by technology

Sodium alginate-chitosan polyion complex hydrogel forms a three-dimensional cross-linked network through electrostatic interactions. When used as tissue engineering scaffold materials, the presence of small molecules such as sodium ions and chloride ions in the body will destroy the formation of electrostatic interactions. The three-dimensional cross-linked network leads to the rapid degradation of the hydrogel scaffold material, and the degradation process is gradually degraded from the surface. During the degradation process, the mechanical properties of the hydrogel material decrease rapidly, and it is not conducive to the adhesion and proliferation of cells cultured on the surface of the material.

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