Preparation method and application of several thrombolytic compounds produced by polar marine microorganisms

A compound and thrombolytic technology, applied in the direction of active ingredients of hydrocarbon compounds, drug combinations, medical preparations containing active ingredients, etc., to achieve high specificity and low blood risk

Active Publication Date: 2021-01-29
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Typical fibrinolytic therapy clinically used drugs such as tissue-type plasminogen activator, urokinase-type plasminogen activator, etc. will bring a significant risk of bleeding and can only be used to treat patients with severe thrombosis

Method used

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  • Preparation method and application of several thrombolytic compounds produced by polar marine microorganisms
  • Preparation method and application of several thrombolytic compounds produced by polar marine microorganisms
  • Preparation method and application of several thrombolytic compounds produced by polar marine microorganisms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Activation of strains

[0039] Use YM medium to activate the Geomyces pannorum strain, add 50mL of YM medium to a 250mL conical flask, sterilize the medium with a high-pressure steam sterilizer at 121°C and 0.1MPa for 15 minutes, and then cool to room temperature . The bacteria were inoculated into the Erlenmeyer flask containing the medium, and the inoculation amount was 2% (1 mL) of the fermentation broth. Under the condition of 180rpm and 25°C, the shake flask was activated for 3 days, and then the activation was stopped.

[0040] YM medium: yeast extract 3g, wort juice 3g, peptone 5g, glucose 20g, distilled water 1L.

[0041] 2. Strain fermentation

[0042] After the activation, the modified Chase medium was used for fermentation. Add 50 mL of modified Chapeauer's medium into a 250 mL conical flask, sterilize the medium at 121° C. and 0.1 MPa for 15 minutes in a high-pressure steam sterilizer, and then cool to room temperature. Inoculate the strains into the E...

Embodiment 2

[0067] Take 1 mg of each compound 1-6 prepared in Example 1 and dissolve them in 0.5 mL of DMSO, add water for injection as usual, fine filter, seal and sterilize to make an injection.

Embodiment 3

[0069] Take 1 mg of each compound 1-6 prepared in Example 1 and dissolve them in 0.5 mL of DMSO, add water for injection as usual, dissolve it in sterile water for injection, stir to dissolve, and filter with sterile suction Filter through a funnel, pack in ampoules, freeze-dry at a low temperature, and then aseptically melt seal to obtain a powder for injection.

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Abstract

The invention discloses the preparation method and application of several thrombolytic compounds produced by polar marine microorganisms; the Geomyces pannorum bacterial strain is activated by adopting YM medium; ; add methanol equal to the volume of the modified Chase medium, sonicate and centrifuge, take the supernatant, concentrate it under reduced pressure, and dry it in vacuum; the obtained dry solids are eluted by gradient chromatography on a silica gel column to obtain compounds 1-6 respectively. Compound 1‑6 showed thrombolytic activity, and the EC of the evaluation system 50 They were 300, 120.0, 181.3, 300, 200.0 and 179.7 μg / mL, respectively. The compound 1‑6 having thrombolytic activity can be used as a thrombolytic drug to treat or prevent thromboembolic diseases.

Description

technical field [0001] The invention relates to the preparation method and application of several thrombolytic compounds produced by polar marine microorganisms. Background technique [0002] Thrombotic disease is one of the three major diseases that cause human death, which seriously threatens human life and health. The number of deaths caused by thrombotic disease has exceeded that of malignant tumors. Thrombolytic therapy is an effective treatment for early acute myocardial infarction and thromboembolic disease. Fibrinolytic therapy is an effective treatment for early acute myocardial infarction and other thrombotic diseases. This therapy uses drugs to activate the potential active plasminogen in the blood to form active plasmin to degrade thrombus fibrin degradation products and dissolve thrombus. . Fibrinolytic therapy is known as one of the top ten discoveries in the field of cardiovascular and cerebrovascular pharmacology in the 20th century. [0003] There are nea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P5/02C12P7/64C12P7/62C12P33/20A61K31/19A61K31/192A61K31/01A61K31/58A61P7/02
CPCA61K31/01A61K31/19A61K31/192A61K31/58C12P5/026C12P7/62C12P7/6436C12P33/20A61K2300/00Y02E50/30
Inventor 郭锐华段东钱士云包斌张朝燕吴文惠
Owner SHANGHAI OCEAN UNIV
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