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Composition used for relieving ratio imbalance of T lymphocyte subpopulation and product applying composition

A technology of lymphocytes and compositions, applied in the field of medicine, can solve the problems of lack of effective treatment of ulcerative colitis, etc., and achieve the effect of improving the imbalance of T lymphocyte subsets, low side effects and good curative effect

Inactive Publication Date: 2017-12-22
津药达仁堂集团股份有限公司乐仁堂制药厂
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The first object of the present invention is to provide a composition containing rhubarb extract and croton cream in the preparation of products that improve the expression of T lymphocyte subsets, the preparation of products that activate T cell immune responses, or the preparation of protection and / or intervention of mucosa The second object of the present invention is to provide a drug for improving the imbalance of T lymphocyte subsets, activating T cell immune response or protecting and / or intervening in mucosal barrier damage, To alleviate the technical problems in the prior art that there is a lack of effective drugs for the treatment of ulcerative colitis and the existing drugs have serious adverse reactions

Method used

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  • Composition used for relieving ratio imbalance of T lymphocyte subpopulation and product applying composition
  • Composition used for relieving ratio imbalance of T lymphocyte subpopulation and product applying composition
  • Composition used for relieving ratio imbalance of T lymphocyte subpopulation and product applying composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Example 1 Dosing Concentration Calculation and Main Reagent Preparation

[0078] Dosing concentration

[0079] Dosage of rhubarb polysaccharide: 400 mg / (kg×d) for high dose, 300 mg / (kg×d) for medium dose, and 200 mg / (kg×d) for low dose.

[0080] Dosage of croton cream: 134mg / (kg×d)

[0081] Sulfasalazine tablets (Salazosulfapyridine, SASP), administered at 500mg / (kg×d).

[0082] Preparation of main reagents

[0083] 3% ethanol solution of picric acid: take 3g of picric acid and place it in a 100mL volumetric flask, add 30mL of 70% ethanol, shake to dissolve, and finally add distilled water to make up to 100mL. TNBS enema solution: first mix equal volumes of absolute ethanol and sterilized physiological saline to prepare a 50% (V / V) ethanol solution, then mix equal volumes of 5% TNBS stock solution and 50% ethanol solution to prepare a solution containing 25g / L TNBS and 250mL / L ethanol enema solution. 0.5% CMS solution: Take 1g of CMS and put it in a beaker, add dis...

Embodiment 2

[0085] Example 2 Model Preparation and Administration Grouping

[0086] The experimental rats were marked with picric acid 24 hours before modeling and grouped according to the random number table, 10 in each group, a total of 6 groups, respectively normal control group (N), model control group (C), croton Cream + rhubarb polysaccharide high (B+R80), medium (B+R60), low (B+R40) dose groups, sulfasalazine group (positive drug). All animals were fasted and watered, and were anesthetized by intraperitoneal injection of 10% chloral hydrate (3mL / kg) on ​​the day of modeling, placed in the rat holder in an upside-down position, and the modified 8FR catheter (along the catheter) The end of the urinary catheter was cut upward about 12 cm, and the metal needle of the 5mL syringe needle was partly pulled out, and the remaining plastic part was bonded to the intercepted part of the urinary catheter, and then the junction was tightly wrapped with PTFE tape to ensure its airtightness. Mak...

Embodiment 3

[0096] Embodiment 3 Specimen collection method

[0097] After 7 days of administration, the experimental rats in Example 2 and Comparative Examples 1 to 4 were fasted for 24 hours and anesthetized by intraperitoneal injection of 10% chloral hydrate (3 mL / kg). Anesthetized rats were placed in a supine position on the rat board, and one side of the groin was selected for skin preparation, and the femoral artery was exposed. The femoral artery was cut off and 2 mL of blood was taken quickly, anticoagulated with EDTA, and stored at 4°C. Immediately after blood collection, the colon tissue was dissociated, cut open along the longitudinal axis of the mesentery, washed three times in 4°C normal saline, the excess water was absorbed by filter paper, the general shape and damage of the colon were observed and recorded, scored, and photographed, and the colon tissue at the ulcer lesion was intercepted Place about 1 cm in 10% neutral formaldehyde solution for paraffin-embedded sections; ...

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Abstract

The invention provides a composition used for relieving ratio imbalance of T lymphocyte subpopulation and a product applying the composition, and relates to the technical field of medicines. A composition of a rhubarb extract and defatted croton seed powder can effectively stop diarrhea. Proved by experiments, the composition of the rhubarb extract and the defatted croton seed powder can relieve the ratio imbalance of the T lymphocyte subpopulation and can activate T cell immune response, so that mucosal barrier injury is protected and / or intervened, and therefore, another evidence is provided for enabling the composition of the rhubarb extract and the defatted croton seed powder to realize mucosal barrier injury repair, and a potential value is achieved for preventing and / or treating ulcerative colitis. The medicine provided by the invention is prepared from the rhubarb extract and the defatted croton seed powder, can be used for relieving the ratio imbalance of the T lymphocyte subpopulation, activating T cell immune response or protecting and / or intervening mucosal barrier injury, and has the advantages of good curative effect and low side effect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a composition for improving the imbalance of T lymphocyte subsets and products using the composition. Background technique [0002] Ulcerative colitis (UC) is a chronic nonspecific colonic inflammation that is idiopathic to the colon and mainly infiltrates the mucosa and submucosa, and belongs to the category of "diarrhea" in traditional Chinese medicine. The etiology and pathogenesis of this disease are not yet very clear, and it is related to susceptibility genes, environmental factors and abnormal immune system. However, more and more studies have shown that the disease is related to the abnormal function of the intestinal mucosal immune barrier and the resulting intestinal mucosal immune imbalance. [0003] The imbalance of the intestinal immune system is an important mechanism for intestinal mucosal tissue damage. The intestinal epithelial barrier is destroyed, the permeab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/708A61K36/47A61P37/04A61K31/715
CPCA61K31/715A61K36/47A61K36/708A61K2300/00
Inventor 王磊王琳金兆祥张宇韩立云王玉晶
Owner 津药达仁堂集团股份有限公司乐仁堂制药厂
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