Preparation method of light-harvesting and oxygen-releasing paper coating material
A coating material and paper-based technology, applied in the field of preparation of bionic paper-based coating materials, can solve the problems of complex reagent selection, cumbersome experimental operation process, no application, etc., and achieve strong operability, improve light-harvesting performance, Reduce the effect of photoinhibition
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Embodiment 1
[0021] Weigh 20 g of fresh spinach leaves, cut them into pieces, put them into a mortar, add 60 mL of ice-cold extract (0.05 mol / L pH=7.6 phosphate buffer solution containing 0.4 mol / L sucrose, 0.1 mol / L sodium chloride) and quickly grind 2 Slurry in minutes, filter with 6 layers of gauze, centrifuge the filtrate at 300r / min for 5 minutes, discard the precipitate, and centrifuge the supernatant at 1000r / min for 5 minutes to obtain complete chloroplasts; take 5mg of chloroplasts and add them to 4mL 0.05mol / L pH=7.6 Phosphate buffered saline solution, mix well to obtain chloroplast suspension; weigh 2×10 -4 Add 1 mL of P25 nano-titanium dioxide powder with a particle size of 25 nm to 1 mL of deionized water, and ultrasonically disperse at room temperature for 60 minutes to obtain a titanium dioxide aqueous solution; fully mix 4 mL of chloroplast suspension and 50 μL of titanium dioxide aqueous solution, and the mass of chloroplasts and titanium dioxide in the resulting mixture is...
Embodiment 2
[0023] In this example, 4 mL of chloroplast suspension and 100 μL of titanium dioxide aqueous solution were thoroughly mixed, and the mass ratio of chloroplast to titanium dioxide in the resulting mixture was 1:4×10 -6 , the other steps were the same as in Example 1 to obtain a light-harvesting and oxygen-releasing paper coating material.
Embodiment 3
[0025] In this example, 4 mL of chloroplast suspension and 150 μL of titanium dioxide aqueous solution were thoroughly mixed, and the mass ratio of chloroplast to titanium dioxide in the resulting mixture was 1:6×10 -6 , the other steps were the same as in Example 1 to obtain a light-harvesting and oxygen-releasing paper coating material.
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