Soluble microneedle for delivering proteins or peptides
A protein and microneedle technology, applied in the field of soluble microneedles for protein or peptide delivery, can solve the problems of increased molecular weight of peptides and difficulty in improving skin absorption
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[0072] In order to facilitate understanding of the present invention, examples are given below in detail. However, various modifications can be made to the examples of the present invention, and the scope of the present invention is not limited to the following examples. The embodiments of the present invention are provided to more completely describe the present invention to those skilled in the art.
[0073]
[0074] First, 1 g of polylactic-co-glycolic acid (PLGA) was dissolved in 10 mL of methylene chloride (MethyleneChloride). A solution of 200 mg of polypeptide (epidermal growth factor, EGF) dissolved in 2 mL of purified water was slowly added to the PLGA solution to make the first W / O emulsion. The W / O emulsion produced for the first time was added to a 0.2% polyvinyl alcohol aqueous solution (100 mL) with stirring. The thus-produced W / O / W double emulsion was stirred at room temperature for 24 hours, methylene chloride as an organic solvent was evaporated, and EGF-l...
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