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Dwarf peony 2n pollen inducing method

A peony and pollen technology, applied in botany equipment and methods, angiosperms/flowering plants, chemicals for biological control, etc., can solve the problems of low proportion of 2n pollen naturally occurring, unable to meet the needs of ploidy breeding, etc. The method is simple and convenient, the probability of occurrence is increased, and the induction efficiency is high

Active Publication Date: 2017-12-01
HENAN INST OF SCI & TECH
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Studies have shown that under natural conditions, only 1.5% of peony varieties can produce more than 1% of 2n pollen, and the proportion of 2n pollen in most excellent varieties is extremely low, which cannot meet the needs of ploidy breeding

Method used

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  • Dwarf peony 2n pollen inducing method
  • Dwarf peony 2n pollen inducing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Induction of 2n pollen of safflower dwarf peony

[0020] Taking the diploid safflower dwarf peony as the test material, from March 12 to 14, 2017, the flower buds in the red dew stage were selected from the 10-20-year-old robust dwarf peony plants, the anthers were stripped, and the pollen mother cells were determined by microscopic examination. Pachytene to metaphase I of meiosis. From 7:30 to 40 in the morning, disinfect the top of the flower buds with absorbent cotton soaked in 75% alcohol for 30 seconds. Use a 1ml syringe to inject the doubling drug solution (5g / L dimethyl sulfoxide + 25g / L colchicine) into the flower buds for three consecutive days and inject once a day. After the syringe is pulled out each time, the pinhole is sealed with paraffin .

[0021] In the bud opening stage, the treated dwarf peony flower buds were brought back to the laboratory and the petals were peeled off. The mature pollen was quickly peeled off with a dissecting needle, ...

Embodiment 2

[0022] Example 2 Successfully obtained 2n pollen of white-flowered dwarf peony

[0023] The specific implementation method is the same as in Example 1, and microscopic examination finds that the proportion of 2n pollen reaches 20.6% on average, and the highest reaches 34.0%, and the vigor of 2n pollen is 91% on average. figure 2 .

[0024] The above results of implementation and application on two representative dwarf peonies show that this method is an effective method for inducing 2n pollen of dwarf peonies. The method is simple and convenient to operate and has a high rate of 2n pollen acquisition.

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Abstract

The invention relates to a dwarf peony 2n pollen inducing method which includes the steps: selecting flower buds of 10-20 perennial healthy and strong dwarf peony plants in a red appearing period; disinfecting the flower buds at 7-8 o'clock in the morning and then injecting 5-7g / L dimethyl sulfoxide and 20-30g / L colchicine into the disinfected flower buds by a syringe once a day in three continuous days; sealing a needle hole by paraffin after pulling out the syringe every time. The inducing method is applied to induction of high-activity dwarf peony 2n pollen, simple, convenient and high in inducing efficiency.

Description

technical field [0001] The invention belongs to the field of plant breeding, and in particular relates to a method for inducing 2n pollen of dwarf peony. Background technique [0002] dwarf peony ( Paeonia jishanensis ) is a precious germplasm resource unique to China (Fu Liguo, 1992). It is only distributed sporadically in Yongji, Shanxi, Yan'an, Shaanxi, and Taihang Mountains, Henan. One of (Zhou Zhiqin et al., 2003). The natural triploid peony 'Shouanhong' has the characteristics of tall plant type, thick branches, thick leaves, and strong stress resistance. It is an indispensable fine variety for the cultivation of major peony gardens. [0003] The ways to obtain plant triploid mainly include natural selection, hybridization between tetraploid and diploid, endosperm culture, hybridization after induction of 2n gametes and so on. Natural screening Due to the low frequency of natural mutations, it is difficult to screen triploids (Su Jing et al., 2012). The use of dip...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H5/02A01N41/10A01N37/18A01P21/00
CPCA01H1/08A01H5/02A01N37/18A01N41/10
Inventor 贾文庆何松林陈韵王艳丽郭英姿杜晓华朱小佩刘会超穆金艳陈晓叶
Owner HENAN INST OF SCI & TECH
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