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Preparation method and application of ChOx ChE/GQDs/N F TNs photochemical biosensor

A biosensor, photoelectrochemical technology, applied in the field of electrochemical analysis, to achieve the effect of improving photocurrent

Inactive Publication Date: 2017-11-28
YUXI NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It can be used to study the damage of ChOx-ChE enzyme activity; it can be used to detect the influence of rutin and oleanolic acid on the activity of cholesterol oxidase, and it provides important information for the research and treatment of diseases caused by high cholesterol and enzyme biosensing technology. in accordance with

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  • Preparation method and application of ChOx ChE/GQDs/N F TNs photochemical biosensor
  • Preparation method and application of ChOx ChE/GQDs/N F TNs photochemical biosensor
  • Preparation method and application of ChOx ChE/GQDs/N F TNs photochemical biosensor

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[0027] A method for preparing a ChOx-ChE / GQDs / N-F-TNs photoelectrochemical biosensor, characterized in that it comprises:

[0028] 1) Preparation of N / F / TiO by electrochemical anodic oxidation 2 NTs electrode

[0029] The titanium sheet is polished and cleaned, ultrasonically cleaned with acetone, absolute ethanol, and deionized water in sequence, soaked in a mixture of hydrofluoric acid and nitric acid, rinsed with acetone and deionized water, and dried under a nitrogen atmosphere. The cathode, the titanium sheet is the anode, and the applied voltage is 20V to contain NH 4 F's (NH 4 ) 2 SO 4 The solution is an electrolyte, which reacts in an electrochemical reactor and is calcined to obtain N / F / TiO 2 NTs electrode;

[0030] 2) Preparation of GQDs by pyrolysis method and modification of N-F-TNs electrodes

[0031] N / F / TiO 2 The NTs electrode was immersed in the solution containing GQDs, soaked for 10min, rinsed with double distilled water, and dried at room temperature...

Embodiment 1

[0041] Preparation of ChOx-ChE / GQDs / N-F-TNs and ChOx-ChE / N / F / TiO 2 NTs photoelectrochemical biosensor

[0042] Step 1: Preparation of N / F / TiO by Electrochemical Anodization Technology 2 NTs electrode. Before anodizing, the titanium sheet is first polished with different sandpapers, then immersed in distilled water for ten minutes and ultrasonically cleaned with acetone, absolute ethanol, and double distilled water in turn, and then the clean titanium sheet is immersed in hydrofluoric acid. In the mixture of acid and nitric acid for 1 minute, the volume ratio of the mixture is HF:HNO 3 :H 2 O=1:4:5, after which the titanium piece was rinsed with acetone and distilled water, and dried at room temperature.

[0043] TiO 2 NTs electrodes were prepared in a columnar electrochemical reactor (radius 30mm, height 70mm). Throughout the preparation process to contain 0.1M NH 4 1M of F (NH 4 ) 2 SO 4 The solution was used as the electrolyte, the graphite electrode was used as th...

Embodiment 2

[0049] ChOx-ChE / GQDs / N-F-TNs and ChOx-ChE / N / F / TiO 2 Photocurrent Response of NTs Photoelectrochemical Biosensor to Cholesterol

[0050] With ChOx-ChE / GQDs-N / FTiO prepared in embodiment 1 2 NTs or ChOx-ChE / N / F / TiO 2 The NTs photoelectrochemical biosensor is the working electrode, the silver silver chloride electrode is the reference electrode, and the platinum wire is the auxiliary electrode. The voltage of the working electrode in the photoelectrochemical cell is set to +0.6V in a phosphate buffer solution with pH=7.00 , with a 500W xenon lamp as the light source and 600nm wavelength light as the excitation light source, the photocurrent response value after adding different concentrations of cholesterol was recorded by chronoamperometry.

[0051] figure 1 Shown is the photocurrent map of modified graphene quantum dots, enzymes and cholesterol electrodes during the preparation of ChOx-ChE / GQDs / N-F-TNs photoelectrochemical biosensor. figure 1 Among them, curve a is the phot...

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Abstract

The invention discloses a preparation method and application of a ChOx ChE / GQDs / N F TNs photochemical biosensor. The preparation method is characterized by comprising the steps of (1) preparing an N / F / TiO2NTs electrode by an electrochemical anodic oxidation method; (2) preparing GQDs by a high-temperature pyrolyzing method, and modifying an N F TNs electrode; (3) building a ChOx ChE / GQDs / N F TNs photochemical biosensor by a crosslinking method. The ChOx ChE / GQDs / N F TNs photochemical biosensor provided by the invention can generate photocurrent under the visible-light excitation; cholesterol (CHO) is used as a substrate; Ch and CE obtained through ChOx ChE catalytic hydrolysis can capture photogenerated holes to inhibit the compounding of photogenerated holes and photo-induced electrons, so that the photocurrent is improved. The ChOx ChE enzymatic activity damage can be studied; the photochemical biosensor can be used for detecting the influence of rutin and oleanolic acid on the cholesterol oxidase activity; important basis is provided for the enzyme biosensing technique aspect and study and treatment methods on diseases caused by high cholesterol.

Description

technical field [0001] The invention relates to the technical field of electrochemical analysis, in particular to a method for preparing a ChOx-ChE / GQDs / N-F-TNs photoelectrochemical biosensor and its application. Background technique [0002] Cholesterol, also known as cholesterol, is a derivative of cyclopentane polyhydrophenanthrene. Cholesterol is abundant in animals, especially in nerve tissue and brain, and also in kidney, liver, bile, skin and spleen. The solubility of cholesterol is similar to that of fat, insoluble in water, but easily soluble in organic solvents such as chloroform and ether. For humans, the total cholesterol in the body is mainly composed of free cholesterol and esterified cholesterol. The normal cholesterol concentration range in serum is 140-190mg / dL. When the concentration of cholesterol in serum is too high, it will cause diseases such as atherosclerosis, heart disease, diabetes, etc. Therefore, the determination of cholesterol in serum has be...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 黄齐林黄旭花李明史云东林洪周元清曾艳萍吴云英仲一卉
Owner YUXI NORMAL UNIV
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