A porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virion vaccine and its preparation method and application

A swine flu virus, respiratory syndrome technology, applied in the direction of virus/phage, biochemical equipment and methods, virus, etc., can solve the problem of reduced effect, reduced active ingredients of vaccine, difficult to maintain the immunogenicity and safety of inactivated vaccine, etc. problems, to achieve the effect of enhanced presentation, prolonged vaccine immunization period, and enhanced local and systemic immune responses

Active Publication Date: 2020-08-11
CHINA AGRI VET BIO SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Highly purified vaccine antigens, because the vaccine formula contains trace amounts of residual impurity proteins and nucleic acids, which eliminates potential allergic reactions, but on the other hand, it will cause degradation, adsorption or aggregation of purified inactivated viruses, thereby reducing the active components of the vaccine and reducing the effect , so excipients need to contain stable adjuvants and antigen stabilizers. At present, it is difficult to maintain the immunogenicity and safety of inactivated vaccines with single aluminum-stabilized adjuvants and two-way oil-stabilized adjuvants for vaccines. Therefore, it is necessary to develop A stable adjuvant for long-term vaccines that can induce specific humoral and cellular immunity

Method used

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  • A porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virion vaccine and its preparation method and application
  • A porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virion vaccine and its preparation method and application
  • A porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virion vaccine and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Example 1 Preparation of porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virus particles and vaccines

[0086] 1. Virus strain, cell, culture medium

[0087] The vaccine and the PRRSV antibody strain prepared by the invention are the 25th generation virus strain HP-PRRSV-JXM-F25 of the highly pathogenic porcine reproductive and respiratory syndrome virus HP-PRRSV-JXM-F5 strain which is passed on Marc-145 cells. The HP-PRRSV-JXM-F5 strain of the highly pathogenic porcine reproductive and respiratory syndrome virus, which is the adapted strain of HP-PRRSV-JXM uploaded to the 5th generation in MARC-145 cells, is classified and named as the highly pathogenic porcine reproductive and respiratory syndrome virus. Respiratory Syndrome Virus, its microbial deposit number is: CGMCCNO.9453, deposited in the General Microbiology Center of the China Microorganism Culture Collection Administration Committee, the preservation date is July 8, 201...

Embodiment 2

[0151] Example 2 Preparation and quality control method of stable adjuvant complex, porcine reproductive and respiratory syndrome whole virus-swine influenza reconstituted virion vaccine

[0152] 1. Preparation and assay of BCG lysate

[0153] The strain Bacillus Calmette-Guérin (BCG) D2BP302S11 was provided by the China Institute for the Control of Drugs and Biological Products, and the seed batch was established according to the strain management regulations for the production and verification of biological products. It should not exceed 12 generations from the batch of working seeds to the collection of bacterial cells. BCG should float on the surface of Sutong medium, which is a wrinkled and yellow bacterial membrane. The results of virulence test and non-toxic BCG test were negative. Freeze-dried strains are stored at 2-8°C, and liquid strains are stored below -70°C.

[0154] BCG D2BP302S11 was statically cultured at 37°C for 2-3 weeks in modified Sutong comprehensive m...

Embodiment 3

[0184] Example 3 Stability and validity of porcine reproductive and respiratory syndrome intact virus-swine influenza virus reconstituted virion vaccine

[0185] 3.1 Stability of vaccine semi-finished products in liquid frozen state

[0186] The porcine reproductive and respiratory syndrome intact virus-swine influenza virus reconstituted virion vaccine stock solution was prepared according to the method in Example 1, and the stable adjuvant complex and the preparation method of the vaccine antigen were prepared according to the method in Example 2.

[0187] The semi-finished vaccines were stored in polypropylene bottles at -35°C and -70°C, and the inactivated and purified porcine reproductive and respiratory syndrome intact virus-swine influenza virus reconstituted virion particles were detected every 3 months. The results are shown in Table 1 below.

[0188] Table 1 Stability of semi-finished vaccines stored in frozen state

[0189]

[0190]

[0191] The results in Ta...

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Abstract

The invention discloses a porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion vaccine as well as a preparation method and an application thereof. The vaccine consists of porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion antigen and a stable adjuvant compound; a porcine reproductive and respiratory syndrome virus-swine influenza virus reconstructed virion is formed through direct adsorption or covalent binding of porcine reproductive and respiratory syndrome virus to the surface of a swine influenza virus reconstructed virion, wherein the swine influenza virus reconstructed virion is a small vesicle containing a phospholipid bilayer; and haemagglutinin and neuraminidase are bound to the surface of the phospholipid bilayer. The vaccine prepared by the invention has the characteristics of being safe, efficient, convenient to use, long in efficacy time and the like. Purposes of reducing and / or removing respiratory syndrome virus and / or swine influenza virus can be achieved through intranasal immunization.

Description

technical field [0001] The invention relates to a porcine reproductive and respiratory syndrome virus-swine influenza virus reconstituted virion vaccine and a preparation method and application thereof. The invention belongs to the technical field of biomedicine or veterinary biological products. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) is a chronic disease of pigs. It is prevalent in countries where pigs are raised around the world, causing an annual economic loss of about 40 billion. It is one of the most important infectious diseases in pig breeding and meat industry. . [0003] Since the discovery of PRRSV virus, inactivated and attenuated modified live vaccines (PRRS-MLV) have been developed at home and abroad for PRRSV control. Nonetheless, PRRS-MLV has the risk of recombination mutation in susceptible pigs, and the live vaccine strain also has the possibility of virulence reversion. Although inactivated vaccines are safe, t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/295A61K39/145A61K39/12A61K39/39A61P31/16A61P31/14
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/5258A61K2039/543A61K2039/55583A61K2039/55594A61K2039/57A61K2039/575A61K2039/70C12N2710/10034C12N2760/16034
Inventor 吕宏亮董金杰张杰田波邓瑞雪王会保刘萍刘西兰景志忠高世杰王超英张云德张永光殷宏
Owner CHINA AGRI VET BIO SCI & TECH
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