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A kind of silver nano material and its biological preparation method and application

A bio-preparation and silver nanotechnology, applied in the field of bio-preparation of silver nanomaterials, can solve problems such as difficulty in controlling slow release, easy hydrolysis, and secondary pollution

Active Publication Date: 2021-05-18
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of organic antibacterial materials are poor stability, easy hydrolysis, secondary pollution, toxicity, and difficulty in controlling the slow release

Method used

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  • A kind of silver nano material and its biological preparation method and application
  • A kind of silver nano material and its biological preparation method and application
  • A kind of silver nano material and its biological preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039]The biological preparation of silver nanomaterials, the preparation process is as follows:

[0040]FUSARIUM SOLANI D07 Strain activation → Seed medium → Fermentation culture → suction filtration Getting mycelial → Stereofil in sterile deionized water → suction filtration to obtain filtrate → joose agno3Reaction → material acquisition.

[0041]The specific preparation process includes the following steps:

[0042](1) Take the Fusarium Solani D07 strain of Iron Dendrobium, under aseptic conditions, use a inoculation needle to pick a small amount of mycelium, access the sterilized solid PDA medium test tube, activated in 28 ± 1 ° C 72 hours;

[0043](2) Take activated strains, under sterile conditions, transferred into a sterilized liquid PDB seed medium, 72 hours in 28 ± 1 ° C, 120 rpm shaker, and seed fluid;

[0044](3) Under sterile conditions, in the amount of 10 V% (volume percent), the amount of 500 ml of liquid medium was added to 28 ± 1 ° C, 120 rpm shaker fermentation for 3 days;

[0045]...

Embodiment 2

[0054]The test observation of the nanomaterial obtained by the nanomaterial obtained in Example 1 and the Comparative Example 1, the antibacterial activity evaluation was filtered, the process was as follows:

[0055]Pathogen strain activation → seed liquid culture → plate coating inoculation → silver nano filter paper inoculation → culture → antibacterial effect observation.

[0056]Antibacterial activity is observed, including the following steps:

[0057](1) Seven common pathogenesis: E. coli, Golden Staphylococcus, Bacillus, Salmonella, Crystallobacillus, Tropical Pseudohamethrosis, bacterium bacteria, in sterile conditions, The inoculation needle sends a small number of colonies, access the sterilized beef cream protein medium (solid state) test tube, activated 24 hours at 37 ± 1 ° C;

[0058](2) Take the strains after activation, transferred into a sterile liquid beef paste liquid medium (liquid) in a sterile conditions, 37 ± 1 ° C, 160 rpm shaker culture for 24 hours, Seed fluid;

[0059](3...

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Abstract

The invention discloses a silver nanometer material and its biological preparation method and application. The method uses endophytic fungi isolated from the roots of Dendrobium officinale Fusarium solani D07 (preservation number CCTCC NO.M2017145) biological extracellular synthesis of the silver nanomaterial, the reaction conditions are mild, easy to control, avoiding the toxic or radioactive reagents in chemical and physical methods, and has the advantages of reliability and greenness. The silver nanometer material of the present invention is a round or oval nanoparticle material, has good dispersibility, narrow particle size distribution, particle size of 16-46nm, and good antibacterial effect. Utilizing the good antibacterial activity of the silver nanomaterials, the application of the silver nanomaterials to the preparation of antibacterial materials has good application prospects.

Description

Technical field[0001]The present invention relates to the preparation and application of silver nanomaterials, and more particularly to biological preparation methods and applications of silver nanomaterials.Background technique[0002]Metal nanomaterials have become one of the important directions of people's research in recent years since its inceptions in light electronics, catalytic, sensors, and biological areas. Among them, nano silver is an emerging functional material because of its unique physical chemical properties, it is concerned in the research field of nano-optoelectronic devices, biomarkers, and surface enhanced Raman and biomedicine composites.[0003]The antibacterial material can be divided into three major types of natural, organic and inorganic antibacterial materials. Natural biocolibacterial materials are derived from natural extract, non-toxic or low toxic, but persistent, and are limited by processing conditions, and cannot be the preferred choice for bacteriost...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00A01N59/16A01P1/00C12R1/77
CPCA01N59/16C12P3/00
Inventor 娄文勇王洪峰曾英杰宗敏华蒲磊
Owner SOUTH CHINA UNIV OF TECH
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