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Drug loaded amnion sheet and preparation method and applications thereof

A technology of amniotic membrane and drug loading, which is applied in the directions of pharmaceutical formulation, tablet delivery, drug combination, etc., and can solve the problems of long treatment cycle and insignificant effect

Inactive Publication Date: 2017-11-17
JILIN TUO HUA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, conventional debridement, flushing, removal of necrotic tissue, and external application of various decubitus creams are mainly used for treatment, but the treatment cycle is often long and the effect is not significant. Therefore, it is urgent to develop a new drug for the treatment of skin ulcers

Method used

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  • Drug loaded amnion sheet and preparation method and applications thereof
  • Drug loaded amnion sheet and preparation method and applications thereof
  • Drug loaded amnion sheet and preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: Preparation of drug-loaded amniotic membrane sheet

[0056] 1. Reagents and consumables: see Table 1.

[0057] Table 1

[0058]

[0059] 2. Equipment:

[0060] Freeze dryer (LGJ-10F vacuum freeze dryer; Beijing Songyuan Huaxing Technology Development Co., Ltd.); moisture meter (VM-210 portable moisture meter for chemical raw materials; Taizhou Weikete Instrument Co., Ltd.).

[0061] 3. Method:

[0062] (1) Acquisition of amniotic membrane

[0063] With the informed consent of the parturient (from Siping Central City People's Hospital, Jilin Province), prenatal checks for hepatitis B virus, hepatitis C virus, human immunodeficiency virus, syphilis, cytomegalovirus, Epstein-Barr virus, human T-lymphoblastic leukemia virus, mycoplasma, and chlamydia , the test results were negative. Take the placental tissue from healthy puerpera after full-term delivery, tear the amniotic tissue, use sterile flat forceps to bluntly peel off the chorionic layer, disca...

Embodiment 2

[0072] Embodiment 2: the test of drug-loaded amniotic film

[0073] 1. Sterility test

[0074] With the drug-loaded amniotic membrane prepared in Example 1, use sterile ophthalmic scissors to cut the drug-loaded amniotic membrane with an area of ​​2cm × 2cm into a sterile plate, add 1.5ml sterile water for injection, and cut the amniotic membrane into pieces with ophthalmic scissors. Put it in mud, draw the tissue fluid into a 5ml centrifuge tube with a Pasteur dropper, centrifuge at 800r / min for 5min, take 20μL of the supernatant and apply it on the Columbia blood agar plate with an inoculation loop, and incubate in a 37°C incubator for 72h. Observe whether there are colonies, the culture results show no colony growth, and the sterility test result is negative.

[0075] Randomly extract 10ml of the solvent prepared in Example 1, apply the solution on the Columbia blood agar plate with an inoculation loop, and cultivate it in a 37°C incubator for 72 hours to observe whether t...

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Abstract

The invention relates to a drug loaded amnion sheet and a preparation method and applications thereof. Specifically, the drug loaded amnion sheet comprises a dry amnion and following components, which are loaded on the dry amnion and substrate membrane surface of amnion, in parts by weight: 5 to 30 parts of bletilla striata, 10 to 30 parts of calcined gypsum, 3 to 25 parts of frankincense, 3 to 25 parts of myrrh, 10 to 30 parts of rhizoma atractylodis, 0.5 to 5 parts of artificial musk, 1 to 15 parts of borneol, and 2 to 30 parts of safflower. The invention also discloses a preparation method of the drug loaded amnion sheet and an application of the drug loaded amnion sheet in the preparation of a medical material for treating skin ulcer diseases. The rapid release and slow release functions of the drug loaded amnion sheet are combined, and the drug loaded amnion sheet can sustain an effective drug concentration in the local environment of wounds.

Description

technical field [0001] The invention relates to the field of medical drug-loaded biological amniotic membrane dressings, in particular to a drug-loaded amniotic membrane sheet and a preparation method thereof, and an application of the drug-loaded amniotic membrane sheet in preparing medical materials for treating skin ulcer diseases. Background technique [0002] Human amniotic membrane is the innermost layer of the placenta. It is rich in sources and easy to obtain. It is an ideal bioremediation material. It is excreted with the placenta during childbirth. It is a kind of medical waste, and its research and application will not involve ethical and moral issues. It mainly includes a single layer of amniotic epithelial cells, a thick basement membrane, and an avascular stroma layer. The amniotic basement membrane is the thickest basement membrane in the human body. Its composition is similar to that of the skin and cornea, mainly collagen types IV and VII, and laminar adhesio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/898A61K47/46A61K9/70A61P17/02A61P3/10A61K33/06A61K35/55A61K31/045
CPCA61K9/7007A61K31/045A61K33/06A61K35/55A61K36/284A61K36/286A61K36/324A61K36/328A61K36/898A61K47/46A61K2236/331A61K2300/00
Inventor 田娜钟永强王秀张石林邢亚丽
Owner JILIN TUO HUA BIOTECH
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