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Lactobacillus plantarum and application thereof in preparing vagina antibacterial medicine

A technology of Lactobacillus plantarum and drugs, applied in the field of microorganisms, can solve the problem of not having the ability to inhibit vaginal pathogens, and achieve the effect of superior vaginal epithelial cell adhesion, active and stable biological characteristics

Active Publication Date: 2017-10-27
广东强基药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims to provide a specific Lactobacillus plantarum to solve the technical problem that conventional Lactobacillus plantarum does not have the ability to inhibit vaginal pathogenic bacteria in the prior art

Method used

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  • Lactobacillus plantarum and application thereof in preparing vagina antibacterial medicine
  • Lactobacillus plantarum and application thereof in preparing vagina antibacterial medicine
  • Lactobacillus plantarum and application thereof in preparing vagina antibacterial medicine

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Embodiment 1 (isolation and inoculation, purification, enrichment culture of Lactobacillus plantarum RD-0025 flora)

[0054] 1. Isolation and inoculation of Lactobacillus plantarum RD-0025 flora: Use two sterile cotton swabs to collect the secretions on the side wall of the subject's vagina, and inoculate them at different concentrations in the culture medium containing the prepared MRS medium. dish, and label the information, place the Petri dish in an anaerobic tank, and put CO 2 Put the gas-producing bag in a 37°C incubator and incubate for more than 48 hours.

[0055] 2. Purification and enrichment culture of Lactobacillus plantarum strain RD-0025: Count the colonies according to their different shapes (surface, edge, etc.) and size. Those with the same shape and size are counted as one, and a few colonies are picked from the inoculation loop. Bacteria are inoculated into MRS solid medium according to the "slash method" to obtain isolated and purified single coloni...

Embodiment 2

[0056] Embodiment 2 (identification and preservation of Lactobacillus plantarum RD-0025 bacterial strain)

[0057] 1. Culture characteristics, staining microscopic examination and morphological characteristics: the colonies obtained after culture are as attached figure 1 , the colony is generally white and round, with a diameter of about 3mm, raised in the middle, smooth and dense; the smear of the pure culture of the bacteria is taken for Gram staining, and the results are shown in the attached figure 2 , showing Gram-positive, straight rods with round ends, single, paired or short chain distribution. The results showed that the isolated strains were preliminarily determined to be Lactobacillus.

[0058] 2. Identification of 16SrDNA gene sequence: DNA was extracted with a bacterial genomic DNA extraction kit, and primers 27F (5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (5'-TACGGYTACCTTGTTACGACTT-3') were used for PCR amplification. The PCR product was subjected to gel electrophor...

Embodiment 3

[0071] Embodiment 3 (determination of metabolites of Lactobacillus plantarum RD-0025)

[0072] 1. Determination of lactic acid content in metabolites of Lactobacillus plantarum RD-0025: adopt high performance liquid phase method (0.005M sulfuric acid aqueous solution (0.28ml sulfuric acid (98%)-1000ml water, pH is about 2.1) to cultivate the fermentation broth of this bacterial strain for 24-48h The lactic acid of Lactobacillus delbrueckii (commercially available) is detected, and the result indicates that lactic acid (L / D lactic acid sum) is about 30mg / mL, which is much higher than the lactic acid content (about 15mg / mL) of Lactobacillus delbrueckii (commercially available). For the lactic acid detection spectrum of the present invention, see the attached Figure 4

[0073] 2. Determination of hydrogen peroxide content in the metabolites of Lactobacillus plantarum RD-0025: semi-quantitative determination of hydrogen peroxide was carried out according to the peroxidase method ...

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Abstract

The invention provides lactobacillus plantarum and application thereof in preparing vagina antibacterial medicine. The lactobacillus plantarum is preserved in China General Microbiological Culture Collection Center (CGMCC), and a preservation number of the lactobacillus plantarum is CGMCC No.14111. Research on metabolic performance of the lactobacillus plantarum finds that compared with ordinary lactobacillus plantarum, the lactobacillus plantarum has stronger lactic acid production capacity, very strong hydrogen peroxide generation capacity and very strong bacteriostatic ability on pathogenic bacteria; meanwhile, the lactobacillus plantarum has outstanding vaginal epithelial cell adhesive capacity. Based on newly-found characters, the invention determines new application of the lactobacillus plantarum in preparing the vagina antibacterial medicine. The lactobacillus plantarum can achieve treatment on varieties of bacterial vaginal diseases. The lactobacillus plantarum disclosed by the invention has obvious effect on bacterial vaginal diseases, safety, no toxicity and good stability and can be stored for a long time. The invention further relates to application of the lactobacillus plantarum in medicines for preventing and / or treating gynecological diseases.

Description

technical field [0001] The invention relates to the technical field of microorganisms, and further relates to the regulation of vaginal micro-ecological environment and the treatment of bacterial vaginal diseases, in particular to a kind of Lactobacillus plantarum and its application for preparing vaginal bacteriostatic drugs. Background technique [0002] There are a variety of microorganisms in the vagina of healthy women, and they form a vaginal micro-ecological system that restricts, coordinates and dynamically balances with the host and the environment. The vaginal flora of healthy women is mainly composed of Lactobacillus, including Lactobacillus plantarum, Lactobacillus jansneri, Lactobacillus gasseri, Lactobacillus crispatus, Lactobacillus vaginalis, Lactobacillus rhamnosus, etc. Under normal circumstances, Lactobacillus can protect the vagina. When the Lactobacillus in the vaginal microecology is disturbed, it can lead to the invasion of pathogenic bacteria and caus...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N15/11A61K35/747A61P31/02A61P31/04A61P31/10A61P15/02A61P33/02A61P13/02C12R1/25
CPCA61K2035/115C12N15/11C12N1/205C12R2001/25Y02A50/30
Inventor 姚耀宏李煜龙许锐鹏潘立许少燕黄旭藩赵湘江
Owner 广东强基药业有限公司
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