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Mussel-sourced biological adhesive for stomach recovery and preparation method thereof

A technology of bioadhesive and mussel, which is applied in the field of mussel-derived bioadhesive for gastric repair and its preparation, can solve the problems of use and effect that have not yet been reported in research, and is expensive, and achieves convenient preparation and low cost , good adhesion effect

Active Publication Date: 2017-10-24
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Currently, the synthetic mussel adhesion protein product CELL-TALK extracted from mussels TM Due to the high price of other adhesives, only a very small amount is applied to the coating of various culture plates, or the cultivation of sperm cells, T47D human breast cancer cells and neutrophils, and its use and effect in organ repair have not yet been established. see research report

Method used

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  • Mussel-sourced biological adhesive for stomach recovery and preparation method thereof
  • Mussel-sourced biological adhesive for stomach recovery and preparation method thereof
  • Mussel-sourced biological adhesive for stomach recovery and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] 1) Take live mussels, remove the viscera, remove most of the adductor muscle, leave only the adductor muscle and its ligaments and shells that are tightly adhered to the inner surface of the shell with a thickness of 1-3mm, and use pre-cooled PBS Liquid washing (4 ℃);

[0060] 2) Add 8M urea solution (4°C) to cover the tissue surface, and perform ultrasonic extraction at 4°C for 16 hours;

[0061] 3) Take the supernatant and select a dialysis bag with a molecular weight cut-off of 5KD for dialysis for 6 days;

[0062] 4) After dialysis, the liquid is freeze-dried to obtain the crude extract of the bioadhesive.

[0063] The result is as figure 1 shown. The scallop tissue adjacent to the adhesion surface was detached from the surface of the shell after being treated with 8M urea solution overnight, and the viscous components were preserved in the urea solution. After dialysis and freeze-drying, the obtained freeze-dried powder is the bioadhesive (crude extract).

[0...

Embodiment 2

[0066] 1) Take a living purple mytilus, remove most of the scallop tissue, and only take a small piece near the adhesion surface, the 2mm adductor muscle and its ligament and shell that are closely adhered to the inner surface of the shell, and wash it with cold PBS solution Net (4°C);

[0067] 2) Add 6M urea solution (4°C) to cover the tissue surface, and perform ultrasonic extraction at 4°C for 14 hours;

[0068] 3) Take the supernatant, and select a dialysis bag with a molecular weight cut-off of 5KD for dialysis for 5 days;

[0069] 4) After dialysis, the liquid is freeze-dried to obtain the crude extract of the adhesive;

[0070] 5) Dissolve the crude extract of the binder with deionized water, wherein 50 ml of deionized water is added to dissolve each g of the crude extract of the binder, and bathed in 80° C. water bath for 2 hours;

[0071] 6) Centrifuge at 1500rpm for 15min, and take the supernatant;

[0072] 7) Continue to bathe the supernatant in 80°C water bath f...

Embodiment 3

[0076] 1) Take live mussels, remove most of the scallop tissue (adductor muscle), and only take small pieces near the adhesion surface, 1mm adductor muscle and its ligament and shell, wash with cold PBS solution (4 ℃);

[0077] 2) Add 7M urea solution (4°C) to cover the tissue surface, and perform ultrasonic extraction at 4°C for 15 hours;

[0078] 3) Take the supernatant and dialyze in a 6-8KD dialysis bag for 4 days;

[0079] 4) After dialysis, the liquid is freeze-dried to obtain the crude extract of the adhesive;

[0080] 5) Dissolve the crude extract of the adhesive with deionized water, wherein 30ml of deionized water is added to each g of the crude extract of the adhesive; 80°C water bath for 2 hours;

[0081] 6) Centrifuge at 2000rpm for 10min, and take the supernatant;

[0082] 7) Continue to bathe the supernatant in 80°C water bath for 1 hour, and centrifuge to obtain the soluble fraction;

[0083] 8) Freeze-drying to obtain a water-soluble bioadhesive.

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Abstract

The invention discloses a mussel-sourced biological adhesive for stomach recovery and a preparation method thereof. According to the mussel-sourced biological adhesive, mytilus coruscus or perna viridis which is abound in the southeastern coast of China is used as a material and adductor muscle with the thickness of no more than 4mm and ligaments and shells thereof are innovatively used as extracting objects; the extracting objects are extracted to obtain the biological adhesive. According to the technology disclosed by the invention, 1.06g of a crude extract adhesive and 0.42g of a water-soluble adhesive can be extracted from each 30 mussels averagely; in a whole preparation process, only less urea solution is needed; the mussel-sourced biological adhesive is convenient to prepare and low in cost and the preparation operation is convenient. The mussel biological adhesive prepared by the method disclosed by the invention has the characteristics of high yield, low cost, good biocompatibility, good adhesion effect and the like, also has a relatively good waterproof performance and has a wide market prospect.

Description

technical field [0001] The invention specifically relates to a mussel-derived bioadhesive for gastric repair and a preparation method thereof. Background technique [0002] Marine mussels belong to the class Mollusc Phylum Gill, which is a common organism in coastal and offshore areas. Its silk glands can secrete silk to attach to hard substrates, so that they can still adhere tightly under the washing of huge waves. on the reef without separation. The main component of this silk glue is mussel foot proteins (mefp), which has high strength, high toughness and water resistance. It has been reported that the proteins with adhesion function mainly include mefp-3 and mefp-5. [0003] In recent decades, there have been more and more researches on the imitation remembrane protein adhesive, and it has continued to receive attention. It has no cytotoxicity, no immunogenicity, and adhesive diversity, so it has a very wide range of medical applications. Prospects. At present, BD B...

Claims

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Application Information

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IPC IPC(8): A61L24/00
Inventor 邱小忠邢孟秋宋小萍
Owner SOUTHERN MEDICAL UNIVERSITY
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