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Root medium for pyrus betulaefolia tissue culture seedlings

A technology for rooting medium and tissue culture seedlings, applied in the field of plant tissue culture, can solve problems such as differences in medium formulations and culture methods, taking into account the selection of explants, inconvenience in research and utilization of Duli rootstocks, and improving the rooting rate. and transplanting survival rate, simplifying the operation process, rooting rate and transplanting survival rate high effect

Inactive Publication Date: 2017-10-17
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

These methods have been successfully reported on different plants, but there are great differences in the medium formulations and culture methods used by different plants, which has caused inconvenience to the research and utilization of Du pear rootstock.
[0006] In addition, these commonly used methods only focus on the formulation of the medium and the concentration and ratio of exogenous growth regulators, and do not take the selection of explants into consideration. In fact, plant endogenous hormones also have an effect on the rooting of explants. Considerable impact, because auxin has a positive and negative effect on plants, that is, growth is promoted at low concentrations, and growth is inhibited above the optimal concentration

Method used

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  • Root medium for pyrus betulaefolia tissue culture seedlings
  • Root medium for pyrus betulaefolia tissue culture seedlings
  • Root medium for pyrus betulaefolia tissue culture seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: the rooting of pear tissue culture seedling

[0040] (1) Medium components

[0041] MS basic medium (inorganic salts, trace elements, iron salts, organic elements):

[0042] Inorganic salt: potassium nitrate 38g / L;

[0043] Ammonium nitrate 33g / L;

[0044] Magnesium sulfate heptahydrate 7.4g / L;

[0045] Potassium dihydrogen phosphate 3.4g / L;

[0046] Anhydrous calcium chloride 6.64g / L;

[0047] Trace elements: manganese sulfate monohydrate 16.9g / L;

[0048] Zinc sulfate heptahydrate 8.6g / L;

[0049] Boric acid 6.2g / L;

[0050] Potassium iodide 0.83g / L;

[0051] Sodium manganate dihydrate 0.25g / L;

[0052] Copper sulfate pentahydrate 0.025g / L;

[0053] Cobalt chloride hexahydrate 0.025g / L;

[0054] Iron salt: disodium edetate 7.46g / L;

[0055] Ferrous sulfate heptahydrate 6.91g / L;

[0056] Organic elements: inositol 20g / L;

[0057] Glycine 0.4g / L;

[0058] Niacin 0.1g / L;

[0059] Pyridoxine hydrochloride 0.1g / L;

[0060] Thiamine hydrochlor...

Embodiment 2

[0073] Embodiment 2: The influence of MS inorganic salt on the rooting effect of Du pear tissue culture seedling

[0074] This example is based on the vigorously growing bushy seedlings with a seedling age of 40 days cultured on MS proliferation medium. Cut the stem section of the bushy seedlings of Du pears about 1.5 to 2.5 cm, and keep a leaf and a leaf bud at the top.

[0075] The rooting medium formula is as follows:

[0076] Treatment 1: MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.25mg / L IBA

[0077] Treatment 2: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.25mg / L IBA

[0078] Test method: the spare test-tube plantlet stems are divided into two groups, and parallel experiments are carried out in the rooting medium of the above two different MS inorganic salt contents, specifically: after the test-tube plantlet stems are inoculated into the medium, observe and cultivate, 40 See Table 1 for statistical data after days. See the photo of D...

Embodiment 3

[0082] Embodiment 3: the screening experiment of auxin IBA concentration

[0083] This example is based on the vigorously growing bushy seedlings with a seedling age of 40 days cultured on MS proliferation medium. Cut the stem section of the bushy seedlings of Du pears about 1.5 to 2.5 cm, and keep a leaf and a leaf bud at the top.

[0084] The medium formula is as follows:

[0085] Treatment 1: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.2mg / L IBA

[0086] Treatment 2: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.25mg / L IBA

[0087] Treatment 3: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.3mg / L IBA

[0088] Treatment 4: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.4mg / L IBA

[0089] Treatment 5: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+0.5mg / L IBA

[0090] Treatment 6: 1 / 2MS+0.3g / L activated carbon+7g / L agar powder+20g / L sucrose+1.0mg / L IBA

[0091] Test method: Divide the spare...

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Abstract

The invention provides a root medium for pyrus betulaefolia tissue culture seedlings. The root medium comprises an inorganic salt halved MS medium, 0.25 to 0.5 mg / L of 3-indolebutyric acid, 6 to 9 g / L of agar, 0.3 to 0.5 g / L of active carbon and 15 to 30 g / L of saccharose. The invention also provides a rooting method for the pyrus betulaefolia tissue culture seedlings. The rooting method comprises the following steps: selecting well-grown pyrus betulaefolia tissue culture seedlings, cutting into stems with the lengths of 1 to 2.5 cm, and putting into the root medium for rooting culture to obtain the pyrus betulaefolia tissue culture seedlings. In combination with explant material drawing manner and matching of exogenous auxin, the efficient and simple root medium for the pyrus betulaefolia tissue culture seedlings is explored, and compared with relatively conventional methods, the root medium is high in rooting percentage and high in survival rate of transplanting, simple and convenient in operation and low in cost, and is applicable to pyrus betulaefolia tissue culture rapid propagation.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a rooting medium for tissue cultured seedlings of Du pear, and also relates to a method for rooting tissue cultured seedlings of Du pear. Background technique [0002] Pear is a plant of the genus Pyrus L. in the subfamily Pomoideae of the Rosaceae family. Pear is an important fruit tree, ranking third in my country's fruit production, after apples and citrus. Pears are propagated by grafting in production, and rootstock is an important basis for fruit tree grafting and is closely related to the production of fruit trees. The selection of excellent rootstocks is very important for regulating tree vigor, prolonging the fruiting period, and even regulating fruit quality. Du pear (Pyrus betulaefolia Bunge.) is the rootstock with the strongest comprehensive resistance among pear tree rootstocks, and plays an important role in the research of pear tree produ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 马媛春王影蔺经常有宏
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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