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Paper chip for quick flow guidance and substance transportation, manufacturing method and application thereof

A microfluidic chip, physical method technology, applied in chemical instruments and methods, laboratory containers, material analysis by observing the effect on chemical indicators, etc., can solve the problem that wax-based chips cannot be bent and cannot be prepared. The problems of structure and lamination are not firm, so as to increase the effective transmission distance, reduce the adsorption loss, and speed up the transmission speed.

Inactive Publication Date: 2017-10-10
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of wax-based chips cannot be bent, the layers are not firm, easy to leak, and cannot prepare complex structures.

Method used

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  • Paper chip for quick flow guidance and substance transportation, manufacturing method and application thereof
  • Paper chip for quick flow guidance and substance transportation, manufacturing method and application thereof
  • Paper chip for quick flow guidance and substance transportation, manufacturing method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Manufacture of paper base-clear plastic tape composite microfluidic chip

[0035] Step 1. Use laser engraved filter paper to make paper chips (Whatman medium-speed quantitative filter paper) as the paper base, such as figure 1 shown;

[0036] Step 2. Use a laser engraving machine to engrave a polyethylene terephthalate (PET) plate mold with a width of 0.5mm and a depth of 0.5mm;

[0037] Step 3. Place the main micro-channel area in the middle of the paper chip obtained in step 1 on the groove of the mold, emboss the groove and seal the entire groove with plastic tape;

[0038] Step 4. Use scissors to cut off the excess part of the tape to obtain a paper-based-clear plastic tape composite microfluidic chip.

Embodiment 2

[0039] Example 2: Paper base-clear plastic tape composite microfluidic chip for simultaneous analysis of multiple ions

[0040] Step 1. making the paper chip with the four-order detection area in Example 1;

[0041] Step 2. preparation each concentration is respectively ferric chloride, nickel nitrate, ammonium dichromate, copper sulfate mixed standard solution of 0.1g / L;

[0042] Step 3. prepare the buffer solution of the 3M ammonium acetate of pH 4.5;

[0043] Step 4. prepare the hydroxylamine solution of 0.1g / ml and the 1,10-phenanthroline solution of 8mg / ml with the solution in step 3;

[0044] Step 5. prepare the polyacrylic acid solution of 0.7mg / ml;

[0045] Step 6. Prepare 0.5M NaF solution;

[0046] Step 7. prepare the buffer solution of the 3M sodium acetate of pH 4.5;

[0047] Step 8. preparation mass fraction is the dimethylglyoxime methanol solution of 1%;

[0048] Step 9. Prepare 0.03M ammonia solution;

[0049] Step 10. prepare 0.35mM ammonium cerium nitra...

Embodiment 3

[0059] Example 3: Paper-base-clear plastic tape composite microfluidic chip for simultaneous analysis of multiple biomacromolecular proteins

[0060] Step 1. make the chip with seven-order detection area in embodiment 1;

[0061] Step 2. prepare bovine serum albumin (BSA) phosphate buffer (PBS) solution of 10mg / mL, 20mg / mL, 40mg / mL, 60mg / mL, 80mg / mL, 100mg / mL, 120mg / mL;

[0062] Step 3. Prepare aspartate aminotransferase (AST) phosphate buffer (PBS) of 10U / L, 20U / L, 40U / L, 60U / L, 80U / L, 100U / L, 200U / L, 300U / L, 500U / L solution;

[0063] Step 4. Prepare 50ml of 250mM citric acid buffer solution with pH 1.8 (containing a drop of polyethylene glycol octylphenyl ether (Triton X-100));

[0064] Step 5. Prepare 95% ethanol solution of 6mM tetrabromophenol blue;

[0065] Step 6. Prepare 10% trehalose aqueous solution;

[0066] Step 7. Dissolve 306mg of cysteine ​​sulfinic acid, 34mg of α-ketoglutaric acid, and 1.6mg of ethylenediaminetetraacetic acid (EDTA) in 1ml of 400mM Tris so...

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Abstract

The invention relates to a paper base-transparent plastic adhesive tape compound micro-fluidic chip. The paper base-transparent plastic adhesive tape compound micro-fluidic chip is formed by using paper with grooves as a base material and fitting the paper base material by a transparent plastic adhesive tape. An effective capillary channel can be formed by fitting the grooves using the plastic adhesive tape. The paper fiber capillary action of the chip is kept while macromolecule matters are allowed to be quickly and directly transported by the capillary tube, which has important practical significance in increasing the paper chip transportation efficiency, realizing quantitative detection of the paper chip and detecting the macromolecules, and has higher practical value. The processing manufacturing method of the interface is flexible and the required equipment and raw materials are cheap and easily available, so that the method provided by the invention, compared with the existing manufacturing method for the paper chip hollow capillary tube, has huge cost advantage and can meet the requirements of volume production and disposable use. The invention discloses a base structure of the chip and a preparation method thereof.

Description

technical field [0001] The invention relates to a microfluidic chip, in particular to a microfluidic chip with a capillary channel constructed by a composite of paper and plastic scotch tape. Background technique [0002] Since Paper analytical devices (uPADs) were introduced into the field of microfluidics by Martinez and others in 2007, they have achieved great development in terms of chip materials, chip manufacturing technology, and chip operation methods. . Traditional paper chips are made of paper, which is not only cheap and easy to obtain, but also easy to cut, infiltrate, and print, so it is suitable for various patterning operations, including inkjet printing, engraving printing, and even handwriting. These mature technologies have laid the foundation for the development of paper chips. In addition, the porous structure of paper fundamentally distinguishes paper chips from traditional chips. This feature brings two advantages: First, reagents are more easily adso...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/78
CPCG01N21/78B01L3/5027B01L3/502707B01L2200/10B01L2300/0838B01L2300/12B01L2300/126G01N2021/7756
Inventor 徐静娟上官金文徐碧漪王莎侯蕴轩陈洪渊
Owner NANJING UNIV
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