Method for detecting hemoglobin by using water-soluble orange fluorescence carbon quantum dot probe

A technology of hemoglobin and carbon quantum dots, which is applied in the field of hemoglobin detection, can solve the problems of insufficient accuracy, low sensitivity, and the need to strengthen anti-interference ability, and achieve the effect of accurate and reliable detection and strong anti-interference ability

Active Publication Date: 2017-09-29
呼和浩特金域医学检验所有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The conventional method of detecting hemoglobin mostly adopts colorimetry, but when the colorimetric method detects the concentration of hemoglobin, the sensitivity is not high, the accuracy is not high enough, and the anti-interference ability needs to be strengthened

Method used

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  • Method for detecting hemoglobin by using water-soluble orange fluorescence carbon quantum dot probe
  • Method for detecting hemoglobin by using water-soluble orange fluorescence carbon quantum dot probe
  • Method for detecting hemoglobin by using water-soluble orange fluorescence carbon quantum dot probe

Examples

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Embodiment 1

[0034] A method for detecting hemoglobin using a water-soluble orange fluorescent carbon quantum dot probe, comprising the following steps:

[0035] Step 1. Use a PBS buffer solution with a pH of 7.4 to prepare a water-soluble orange fluorescent carbon quantum dot solution with a concentration of 0.5 mg / mL, and use a PBS buffer solution with a pH of 7.4 to prepare multiple hemoglobin standard solutions with different concentrations. The concentrations of the hemoglobin standard solutions are in order For: 0, 1×10 -8 mol / L, 3×10 -8 mol / L, 6×10 -8 mol / L, 1×10 -7 mol / L, 3×10 -7 mol / L, 5×10 -7 mol / L, 7×10 -7 mol / L, 1.2×10 -6 mol / L, 2×10 -6 mol / L, 3×10 -6 mol / L, 4×10 -6 mol / L, 6×10 -6 mol / L, 7×10 -6 mol / L, 8×10 -6 mol / L, and 1×10 -5 mol / L, measure 16 parts of water-soluble orange fluorescent carbon quantum dot solutions with a volume of 3ml respectively, add a part of hemoglobin standard solution to a water-soluble orange fluorescent carbon quantum dot solution, and ir...

Embodiment 2

[0038] The determination steps are the same as in Example 1, wherein the difference is the preparation method of the water-soluble orange fluorescent carbon quantum dots in step one, the preparation method of the water-soluble orange fluorescent carbon quantum dot solution, the preparation method of the hemoglobin standard solution, and the preparation method containing hemoglobin. The pretreatment of the test solution is different, as follows:

[0039] The preparation method of water-soluble orange fluorescent carbon quantum dots is specifically:

[0040] S1, 2.5g of glucose, 0.8g of glutathione, and 0.5g of β-cyclodextrin were dissolved in 20mL of deionized water, and 1.2g of silica gel solution was added for ultrasonic treatment. After cooling to room temperature, 1.5g of hydrotalcite powder was added to the reaction kettle React at 165°C for 5 hours, cool to room temperature, take the supernatant, filter it through a 0.45 μm microporous membrane, take the filtrate, and dry...

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Abstract

The invention discloses a method for detecting hemoglobin by using a water-soluble orange fluorescence carbon quantum dot probe. The method comprises the following steps: step one, preparing a water-soluble orange fluorescence carbon quantum dot solution, specifically as follows: uniformly mixing a water-soluble orange fluorescence carbon quantum dot and a PBS buffer solution, sealing in a transparent container through which only the orange light can pass; repeatedly freezing and water-batching, irradiating by an ultraviolet ray in the ice water bath at 0 DEG C, standing to normal temperature; adding hemoglobin standard solutions in different concentrations, irradiating exciting light, establishing the relation of the hemoglobin concentration and the fluorescence strength; step two, irradiating exciting light, detecting the fluorescence strength corresponding to the to-be-detected solution containing the hemoglobin, and reading the concentration of the corresponding hemoglobin in the to-be-detected solution according to the relation of the hemoglobin concentration and the fluorescence strength detected in the step one. The method disclosed by the invention has the advantages of being sensitive in detection, strong in anti-jamming capability, and high in detection accuracy.

Description

technical field [0001] The invention relates to the field of hemoglobin detection. More specifically, the present invention relates to a method for detecting hemoglobin using a water-soluble orange fluorescent carbon quantum dot probe. Background technique [0002] Hemoglobin is an indispensable protein in the human body, most of which are stored in red blood cells. It is a tetrameric protein composed of 2 α and 2 β polypeptide chains, and each peptide chain is combined with a heme prosthetic group ( ferrous porphyrin prosthetic group) binding. Studies have shown that hemoglobin is involved in many important activities related to oxygen, energy and metabolism in organisms. Moreover, hemoglobin is not only a redox protein, but also an allosteric protein. The allosteric effect and redox properties of hemoglobin have attracted the attention of many scientific researchers. Moreover, the concentration of hemoglobin in blood is closely related to many diseases related to blood ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 黄珊肖琦杨二利刘义
Owner 呼和浩特金域医学检验所有限公司
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