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Preparation method of laponite nanoparticles ICG (Indocyanine Green)/LAP (Laponite) supporting the ICG

A nanoparticle and indocyanine green technology, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, wave energy or particle radiation treatment materials, etc., to achieve simple preparation methods, mild reaction conditions, improved stability and photothermal The effect of conversion efficiency

Inactive Publication Date: 2017-09-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Consult domestic and foreign related documents or patents, the preparation method of hectorite nanoparticles ICG / LAP loaded with indocyanine green has not been reported

Method used

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  • Preparation method of laponite nanoparticles ICG (Indocyanine Green)/LAP (Laponite) supporting the ICG
  • Preparation method of laponite nanoparticles ICG (Indocyanine Green)/LAP (Laponite) supporting the ICG
  • Preparation method of laponite nanoparticles ICG (Indocyanine Green)/LAP (Laponite) supporting the ICG

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] (1) Dissolve 2 mg of ICG aqueous solution in 1 mL of ultrapure water, and magnetically stir for 5 minutes at room temperature in the dark to make it uniformly dispersed into ICG aqueous solution;

[0053] (2) Add 3 mL of acetate buffer solution with pH=5.0 into 1.5 mL of 6 mg / mL LAP aqueous solution, and then add ICG aqueous solution to LAP aqueous solution drop by drop, so that the mass ratio of LAP to ICG is 9:2. After reacting for 4 hours at room temperature with magnetic stirring in the dark, centrifuge and purify at 8000 r / min for 10 minutes to obtain ICG / LAP nanoparticles.

Embodiment 2

[0055] Get the ICG / LAP prepared in Example 1, the solvent is ultrapure water, carry out XRD test respectively with LAP after freeze-drying, its diffraction peak and peak area all change, see figure 2 .

Embodiment 3

[0057] Take 150 μg / mL ICG / LAP prepared in Example 1, the solvent is ultrapure water, measure its UV-Vis absorption spectrum, it can be seen from the spectrum that its peak shape is consistent and good, see image 3 . And through the UV standard curve quantification, get ICG upload efficiency. Simultaneously measure the ultraviolet-visible absorption spectrum of ICG solution in embodiment 1.

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Abstract

The invention relates to a preparation method of laponite nanoparticles ICG (Indocyanine Green) / LAP (Laponite) supportng the ICG. The preparation method comprises the following steps: dissolving the ICG into ultrapure water and carrying out light-avoiding stirring under the condition of normal temperature to obtain an aqueous solution of the ICG; dropwise adding the aqueous solution of the ICG into an aqueous solution of the LAP, and uniformly mixing; then carrying out room temperature light-avoiding stirring reaction under the condition of a buffer solution, and centrifugally purifying to obtain a finished product. According to the ICG / LAP nanoparticles, stability and light and heat stability of the ICG in the aqueous solution are improved, the photothermal conversion efficiency of the ICG is improved, and a new idea is provided for subsequent application of the ICG in the biological field.

Description

technical field [0001] The invention belongs to the field of preparation of drug loading, in particular to a preparation method of hectorite nanoparticle ICG / LAP loaded with indocyanine green. Background technique [0002] Traditional tumor treatment methods mainly include surgery, radiotherapy, chemotherapy, etc., but all have relatively large side effects. Compared with traditional treatments, photothermal therapy has certain advantages due to less damage to normal cells. Indocyanine Green (ICG) is currently the only near-infrared imaging reagent approved by the U.S. Food and Drug Administration (FDA) for clinical use. It is a three-carbon cyanine dye with a characteristic near-infrared absorption peak. ~845nm. Human tissue has the lowest absorption coefficient in the near-infrared region of 700 to 1100nm, and the light penetrates the deepest into biological tissue. Therefore, ICG is used as a photothermal reagent for photothermal therapy of cancer, which is harmless to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B33/32A61K41/00A61K9/14A61K47/02A61P35/00
CPCA61K9/143A61K41/0052C01B33/32C01P2002/72C01P2002/84C01P2002/88C01P2004/01
Inventor 郭睿徐凡丽史向阳
Owner DONGHUA UNIV
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