Medical acellular dermal matrix and preparation method thereof

Abstract

The invention relates to the field of medical biomaterials, and concretely relates to a medical acellular dermal matrix and a preparation method thereof. The preparation method comprises the following steps: fleshing animal skins, sequentially carrying out disinfecting, cleaning and hypertonic saline treatment, and immersing the treated animal skins in an alkali liquid and hydrogen peroxide mixed solution; and carrying out enzyme digestion treatment on the animal skins in a trypsin solution, and crosslinking collagens by using a crosslinking agent. The treatment of a hypertonic saline treated dermal sheet with the alkali liquid and hydrogen peroxide mixed solution makes protein molecules in the dermal sheet expanded and loosened and hair directly removed, so the manual hair removal process is omitted, the process flow is simplified, the production efficiency is increased, the protection cost is saved, and the used reagents are nontoxic and do not cause environment pollution. The crosslinking treatment is carried out after the enzyme digestion, so destroys of excess digestion the structure and the arrangement mode of the collagens are avoided, and the toughness, the elasticity and the flexibility of the product are improved.

Description

technical field [0001] The invention relates to the technical field of medical biomaterials, in particular to a medical acellular dermal matrix and a preparation method thereof. Background technique [0002] For patients with extensive burns or trauma, skin grafting is the best treatment method, but it is almost impossible to use autologous full-thickness skin grafting for extremely large burn wounds, while autologous microskin or thin skin grafts require dermal scaffolds. The acellular dermis made from human cadaver skin is the most ideal as a dermis substitute, but its application is greatly limited due to ethics and HIV and other virus reasons. At present, according to practice and clinical use, it has been proved that acellular dermal matrix is ​​a new type of wound repair material, and its application field has gradually expanded from the initial treatment of deep burn wounds to plastic surgery, cosmetology, oral cavity, ENT, general surgery and other fields. [0003] ...

AI Technical Summary

Problems solved by technology

At present, there are several methods for the preparation of acellular dermal matrix: 1. Dispase II-Triton method: This method removes cells completely, but it damages the basement membrane greatly, which is not conducive to the adhesion and growth of epithelial cells; 2. Hypertonicity Salt-SDS method: This method retains the basement membrane intact, but the content of fibronectin and elastin in the dermis is high, so it may have high immunogenicity; 3. Hypertonic salt-sodium hydroxide erosion method: the The method uses hypertonic salt to remove the epidermis, and at the same time uses the water absorption effect of alkali ions in sodium hydroxide to deprive the tissue cells of water and dehydrate the cells. This method has a complete structure of collagen fibers and a looser arrangement than normal, but the effect on cell removal There is still room for improvement; 4. The repeated freezing and thawing method, because it is all a physical operation process, takes a long time and a long cycle, and it is basically not used now.

[0004] Among the above methods, the method of action is single, and the treatment time is too short to completely remove the immunogenic substances. If the action time is too long, it will cause damage to the basement membrane and may cause the loss of a complete cytoskeleton

At the same time, manual physical methods to remove pig hair are time-consuming and labor-intensive, taking up a lot of working hours of producers