Method for observing Tilletia controversa Kuhn (TCK) infected Triticum aestivum ovaries

A technology of Tilletia dwarf, wheat, applied in the field of microbiology

Inactive Publication Date: 2017-07-28
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

[0003] However, there is still a lack of a microscopic observation method that can effectively and clearly demonstrate the infection process of T. tritici to the ovary of wheat

Method used

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  • Method for observing Tilletia controversa Kuhn (TCK) infected Triticum aestivum ovaries
  • Method for observing Tilletia controversa Kuhn (TCK) infected Triticum aestivum ovaries
  • Method for observing Tilletia controversa Kuhn (TCK) infected Triticum aestivum ovaries

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experiment example

[0050] Experimental example, the effect comparison of the method of the present invention and the existing method

[0051] Using the method described in Wei Huixin, Gao Li, Shen Huimin, etc., 2016 "Microscopic Observation of the Infection Process of Wheat Dwarf Bacillus in Wheat", the staining and processing methods of the ovary of normal wheat plants were used to observe the obtained results. Laser confocal imaging such as image 3 shown. ( image 3 The images obtained from the wheat ovary Confocal image with an ovary diameter of 600 μm) cannot accurately and clearly display the outline and size of cells in each part of the ovary, and data statistics cannot be performed on the images.

[0052] The method of this experiment is used to observe the separation, staining and processing methods of the ovary of normal wheat plants. The obtained laser confocal imaging is as follows: Figure 4 shown. ( Figure 4 The image obtained from the wheat ovary Confocal image with an ovary...

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Abstract

The invention provides a method for observing TCK infected Triticum aestivum ovaries, and belongs to the technical field of microorganisms. The method comprises the following steps: (1) carrying out inoculation: using TCK to inoculate spikelets of Triticum aestivum at the booting stage; (2) sampling: after inoculating for one week, taking out the spikelet ovaries of Triticum aestivum determined to be infected with TCK and performing immersion in anhydrous alcohol to be stored; (3) entrapping the spikelet ovaries in paraffin and manufacturing into paraffin sections; (4) dyeing and manufacturing into slides: dyeing the ovaries and placing on glass slides; using double-sided adhesive tapes on two sides of each paraffin section on each glass slide respectively for heightening cover glasses and making the cover glasses capable of being fixed with the glass slides by using the double-sided adhesive tapes after being covered, and manufacturing into observation slides; placing the observation slides under a laser scanning confocal microscope (LSCM) to be observed.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a method for observing the infection, onset and damage processes of Triticum dwarfus in the ovary of wheat. Background technique [0002] Wheat dwarf bunt disease (DB) is an important quarantine disease that harms wheat crops in the world, mainly harming wheat (Triticum aestivum), which can cause dwarf plants, increased tillers and other diseases, and is difficult to control. The introduction will bring devastating harm to wheat production, and its teliospores have a fishy odor, which will greatly affect the quality of wheat. The disease risk assessment of wheat dwarf smut shows that 19.3% of winter wheat planting areas in China have natural conditions suitable for the occurrence of the disease, which is a high-risk area. Tilletia controversa Kühn (TCK) belongs to Basidiomycotina, Teliomycetes, Ustilaginales, Tilletiaceae, Tilletiaceae Tilletia. The host ran...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/63G01N1/30
CPCG01N21/63G01N1/30
Inventor 高利李超陈万权刘太国刘博
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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