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Method for separating high-purity oleuropein

An oleuropein, high-purity technology, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems of restricting development, high equipment and process costs, and achieve reduced production costs, simple operation, and separation. Efficient effect

Inactive Publication Date: 2017-07-21
LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent uses supercritical CO 2 For extraction, the cost of equipment and process is relatively high, which restricts its further development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Sample: get 1kg olive leaf, add 15 liters of 90% (volume ratio) ethanol, heat and reflux extraction 2 times, each 2h, after the extracts are combined, ethanol is recovered under reduced pressure to obtain the olive leaf extract.

[0023] 2. Instrument: DE HPCCC Spectrum countercurrent chromatograph, British DE company.

[0024] 3. Preparation method: Mix n-butanol-ethyl acetate-methanol-water according to the ratio of 1:19:1:19 (v:v) thoroughly, then place it in a separatory funnel and let it stand overnight. Release the upper and lower phases respectively and degas them by ultrasonication for 20 minutes. The upper phase is the stationary phase and the lower phase is the mobile phase. First, pump the stationary phase into the spiral tube of the countercurrent chromatograph at a flow rate of 6mL / min. After the spiral tube is completely filled with the stationary phase, turn on the host of the countercurrent chromatograph, slowly adjust the rotational speed of the spir...

Embodiment 2

[0026] 1. Sample: get 1kg olive leaf, add 12 liters of 80% (volume ratio) ethanol, heat and reflux extraction 2 times, each 2h, after the extracts are combined, ethanol is recovered under reduced pressure to obtain the olive leaf extract.

[0027] 2. Instrument: TBE-300B countercurrent chromatograph, Shanghai Tongtian Biotechnology Co., Ltd., China.

[0028] 3. Preparation method: Fully mix n-butanol-ethyl acetate-methanol-water according to the ratio of 1:19:1:19 (v:v), then place it in a separatory funnel and let it stand for stratification. Release the upper and lower phases respectively and degas them by ultrasonication for 20 minutes. The upper phase is the stationary phase and the lower phase is the mobile phase. First, pump the stationary phase into the spiral tube of the countercurrent chromatograph at a flow rate of 20mL / min. After the spiral tube is completely filled with the stationary phase, turn on the host of the countercurrent chromatograph, slowly adjust the ro...

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Abstract

The invention relates to a method for separating high-purity oleuropein by utilizing counter-current chromatography. According to the method, an ethanol solution is adopted to extract an olive leaf extract, and then the olive leaf extract is separated by using the counter-current chromatography as a separating means. The method comprises the steps that n-butyl alcohol, ethyl acetate, methanol and water are prepared into solvent systems of a flow phase and a fixed phase; the separation column of a counter-current chromatograph is fully filled with the fixed phase, and the flow phase is pumped to achieve two-phase balance; after sample feeding of a sample feeding valve, the flow phase is eluted; a target component is collected according to a detector's graph and combining HPLC detection; an oleuropein monomer compound is obtained after fractions containing the oleuropein are mixed and solvents are recovered. By adopting the method, the oleuropein compound having the purity 90% or above can be obtained. The method is simple, convenient and fast, a separating effect is good, the preparation amount is large, sample loss is small, the separating cost is low, the product purity is high, and the whole separating method is suitable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of natural product separation, and in particular relates to a method for separating high-purity oleuropein. Background technique [0002] Oleuropein is an iridoid glycoside compound. Modern pharmacological studies have shown that oleuropein has various activities such as anti-inflammatory, anti-fungal, anti-virus, anti-oxidation, anti-cancer and hypoglycemic. [0003] In recent years, scholars at home and abroad have made beneficial explorations on the extraction and purification methods of oleuropein. For example, the Chinese invention patent titled "A Method for Purifying Oleuropein" (Application No. 201010549802.X) discloses a method for extracting oleuropein from olive leaves: extract the olive leaves with ethanol, and then add Activated carbon decolorizes, recovers ethanol and extracts with n-butanol, recovers solvent from n-butanol extraction phase and separates with preparative high-speed countercurr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08C07H1/06
CPCC07H17/04C07H1/06C07H1/08
Inventor 黄新异邸多隆
Owner LANZHOU INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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